Team:Kyoto/GSDD/Introduction

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#[[Team:Kyoto|Home]]
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#[[Team:Kyoto/Introduction|Gene Switch Depending on Duplication]]
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#[[Team:Kyoto/GSDD|GSDD]]
#Introduction
#Introduction
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==Project1 -- '''Genetic Expression Depending on Cell division''' -- ==
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==Motivation==
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[[Image:Kyoto_GEDD_1.png|200px|thumb|Fig.1]]
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'''In biotechnology''', genes in vectors soon express after transformation. We want the genes to express depending on the number of cell division after transformation. Ultimately, we want to make a system that can control freely the generation of genetic expression after transformation.
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[[Image:figure(motivation).png|480px|thumb|Fig.1]]
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If we can create a bacteria that cures human, and we inject it in human body, it has bad influence to human body that the artificial bacteria lives in human body forever.
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In synthetic biology field, biologists and bioengineers design and construct a variety of gene circuits to solve their facing problems such as medical, environmental, food, and energy problems. However, it is difficult to control the lifespan of these engineered cells that contain synthetic circuits in many cases: the cells tend to repeat cell division and increase their number in the given environment. Moreover, if point mutations or deletions were introduced into such engineered cells, it may cause serious problems to the natural environment, because they may cause the unexpected behavior (biohazard) against natural living systems. This is the big issue in this field. However, it is difficult to invent an effective approach to control the cell fate of the engineered bacteria.  
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However, using in this system, we can create life span of bacteria if a gene that expresses in this system is cytorethal.  
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'''To achieve''' our purpose, our idea is using liner DNA as a vector that product repressor. Liner DNA is replicated incompletely because of the end replication problem. So, liner DNA becomes short by duplication. Through the repetition of duplication, Liner DNA becomes shorter and shorter, and ultimately the region of a gene becomes lost and the expression of the gene becomes silent. Product of repressor becomes silent and repression of a gene in the other plasmid vector becomes lost and the gene becomes expressed.  
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For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits).  
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[[Image:Kyoto_GEDD_2.png|thumb|700px|center|Fig.2]]
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'''The problem''' in this idea is that liner DNA is not stable in cell because of exonuclease　and proteins related to DNA repair. To settle this problem, the end of liner DNA is the repeats of specific protein binding sites. In this idea, specific proteins bind this repeats, and protect liner DNA by exonuclease , and proteins related to DNA repair. So,liner DNA becomes shorter and shorter, and when the repeats of specific protein binding sites
 
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are lost, exonuclease degrade liner DNA and production of repressor becomes silent.   
 
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Now、we make a plan using E.coli and yeast. Figure 2 is the outline of this system in yeast. The proteins protecting the end of liner DNA is GAL4 in yeast, and LACI in E.coli.
 
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Latest revision as of 02:56, 22 October 2009

Motivation

Fig.1

In synthetic biology field, biologists and bioengineers design and construct a variety of gene circuits to solve their facing problems such as medical, environmental, food, and energy problems. However, it is difficult to control the lifespan of these engineered cells that contain synthetic circuits in many cases: the cells tend to repeat cell division and increase their number in the given environment. Moreover, if point mutations or deletions were introduced into such engineered cells, it may cause serious problems to the natural environment, because they may cause the unexpected behavior (biohazard) against natural living systems. This is the big issue in this field. However, it is difficult to invent an effective approach to control the cell fate of the engineered bacteria.

For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits).

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