http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&feed=atom&action=historyTeam:Kyoto/GSDD/Introduction - Revision history2024-03-28T08:19:26ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=166840&oldid=prevTADA at 02:56, 22 October 20092009-10-22T02:56:05Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#[[Team:Kyoto/GSDD|GSDD]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#[[Team:Kyoto/GSDD|GSDD]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Introduction</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Introduction</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Kyoto/topicpath}}</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Kyoto/Leftnavi}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Kyoto/Leftnavi}}</div></td></tr>
</table>TADAhttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=164691&oldid=prevTADA: /* Safety issue */2009-10-22T02:06:40Z<p><span class="autocomment">Safety issue</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Safety issue==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Safety Issue </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.What we did in laboratory was completely free of anything harmful for environment and ourselves.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We disposed of wastes such as biohazardous waste properly, and we were careful with toxic material.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Biohazard was not caused during our experiment.</del></div></td><td colspan="2"> </td></tr>
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</table>TADAhttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=159575&oldid=prevMako: /* Safety issue */2009-10-21T23:26:52Z<p><span class="autocomment">Safety issue</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety issue==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety issue==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard<ins class="diffchange diffchange-inline">.What we did in laboratory was completely free of anything harmful for environment and ourselves.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We disposed of wastes such as biohazardous waste properly, and we were careful with toxic material.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Biohazard was not caused during our experiment</ins>.</div></td></tr>
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</table>Makohttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=152944&oldid=prevMako at 19:52, 21 October 20092009-10-21T19:52:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>==Safety issue<del class="diffchange diffchange-inline">=</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==Safety issue==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td></tr>
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</table>Makohttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=152931&oldid=prevMako: /* Safety issue */2009-10-21T19:51:32Z<p><span class="autocomment">Safety issue</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Safety issue==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">=</ins>==Safety issue<ins class="diffchange diffchange-inline">=</ins>==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td></tr>
</table>Makohttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=152925&oldid=prevMako: /* Safety issue */2009-10-21T19:51:19Z<p><span class="autocomment">Safety issue</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety issue==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety issue==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>Safety Issue </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Safety Issue </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</div></td></tr>
</table>Makohttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=152902&oldid=prevMako: /* Motivation */2009-10-21T19:50:36Z<p><span class="autocomment">Motivation</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Safety issue==</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times. If engineered cells have mutation and increase too much, it may cause the unexpected behavior (biohazard) against natural living systems. Our device certainly contributes to prevention of biohazard.</ins></div></td></tr>
</table>Makohttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=151683&oldid=prevShoTakamori at 19:05, 21 October 20092009-10-21T19:05:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Motivation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Motivation==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:figure(motivation).png|<del class="diffchange diffchange-inline">420px</del>|thumb|Fig.1]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:figure(motivation).png|<ins class="diffchange diffchange-inline">480px</ins>|thumb|Fig.1]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In synthetic biology field, biologists and bioengineers design and construct a variety of gene circuits to solve their facing problems such as medical, environmental, food, and energy problems. However, it is difficult to control the lifespan of these engineered cells that contain synthetic circuits in many cases: the cells tend to repeat cell division and increase their number in the given environment. Moreover, if point mutations or deletions were introduced into such engineered cells, it may cause serious problems to the natural environment, because they may cause the unexpected behavior (biohazard) against natural living systems. This is the big issue in this field. However, it is difficult to invent an effective approach to control the cell fate of the engineered bacteria. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In synthetic biology field, biologists and bioengineers design and construct a variety of gene circuits to solve their facing problems such as medical, environmental, food, and energy problems. However, it is difficult to control the lifespan of these engineered cells that contain synthetic circuits in many cases: the cells tend to repeat cell division and increase their number in the given environment. Moreover, if point mutations or deletions were introduced into such engineered cells, it may cause serious problems to the natural environment, because they may cause the unexpected behavior (biohazard) against natural living systems. This is the big issue in this field. However, it is difficult to invent an effective approach to control the cell fate of the engineered bacteria. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td></tr>
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</table>ShoTakamorihttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=146778&oldid=prevGEN: /* Motivation */2009-10-21T15:33:25Z<p><span class="autocomment">Motivation</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In synthetic biology field, biologists and bioengineers design and construct a variety of gene circuits to solve their facing problems such as medical, environmental, food, and energy problems. However, it is difficult to control the lifespan of these engineered cells that contain synthetic circuits in many cases: the cells tend to repeat cell division and increase their number in the given environment. Moreover, if point mutations or deletions were introduced into such engineered cells, it may cause serious problems to the natural environment, because they may cause the unexpected behavior (biohazard) against natural living systems. This is the big issue in this field. However, it is difficult to invent an effective approach to control the cell fate of the engineered bacteria. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In synthetic biology field, biologists and bioengineers design and construct a variety of gene circuits to solve their facing problems such as medical, environmental, food, and energy problems. However, it is difficult to control the lifespan of these engineered cells that contain synthetic circuits in many cases: the cells tend to repeat cell division and increase their number in the given environment. Moreover, if point mutations or deletions were introduced into such engineered cells, it may cause serious problems to the natural environment, because they may cause the unexpected behavior (biohazard) against natural living systems. This is the big issue in this field. However, it is difficult to invent an effective approach to control the cell fate of the engineered bacteria. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For instance, several synthetic biologists aimed to regulate cell population by introducing a negative feedback loops or the other circuits in cells (REF). As a result, they could control the cell populations in some cases. However, these regulations can not precisely control the temporal gene expression depending on cell division. Thus, we aimed to design and construct a system to control the temporal gene expression depending on cell division without using the other factors (e.g., small molecules such as Tetracycline or complicated genetic circuits ). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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</table>GENhttp://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Introduction&diff=139493&oldid=prevShoTakamori at 07:13, 21 October 20092009-10-21T07:13:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Motivation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Motivation==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:figure(motivation).png|420px|thumb|Fig.1]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:figure(motivation).png|420px|thumb|Fig.1]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In <del class="diffchange diffchange-inline">bioengineering</del>, biologists design gene circuits <del class="diffchange diffchange-inline">as a mean </del>to solve their facing problems such as medical <del class="diffchange diffchange-inline">problems</del>, environmental <del class="diffchange diffchange-inline">problems</del>, food <del class="diffchange diffchange-inline">problems</del>, energy problems <del class="diffchange diffchange-inline">and so on</del>. <del class="diffchange diffchange-inline">Then they suppose future implement like injection into human blood stream</del>, <del class="diffchange diffchange-inline">scattering across natural environment etc. But </del>in many cases <del class="diffchange diffchange-inline">those designed </del>cells repeat cell division and increase <del class="diffchange diffchange-inline">its </del>number in the environment. <del class="diffchange diffchange-inline">And then sometimes they got tiny internal errors like </del>point <del class="diffchange diffchange-inline">mutation </del>into <del class="diffchange diffchange-inline">their genes</del>, <del class="diffchange diffchange-inline">but show us unexpected behavior and </del>cause serious problems to <del class="diffchange diffchange-inline">its </del>environment. This is <del class="diffchange diffchange-inline">one of </del>the big <del class="diffchange diffchange-inline">problems, to solve that, we bioengineers have to make serious efforts</del>. <del class="diffchange diffchange-inline">But </del>it is <del class="diffchange diffchange-inline">quite </del>difficult to <del class="diffchange diffchange-inline">propose </del>an effective <del class="diffchange diffchange-inline">solution in many cases</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In <ins class="diffchange diffchange-inline">synthetic biology field</ins>, biologists <ins class="diffchange diffchange-inline">and bioengineers </ins>design <ins class="diffchange diffchange-inline">and construct a variety of </ins>gene circuits to solve their facing problems such as medical, environmental, food, <ins class="diffchange diffchange-inline">and </ins>energy problems. <ins class="diffchange diffchange-inline">However</ins>, <ins class="diffchange diffchange-inline">it is difficult to control the lifespan of these engineered cells that contain synthetic circuits </ins>in many cases<ins class="diffchange diffchange-inline">: the </ins>cells <ins class="diffchange diffchange-inline">tend to </ins>repeat cell division and increase <ins class="diffchange diffchange-inline">their </ins>number in the <ins class="diffchange diffchange-inline">given </ins>environment. <ins class="diffchange diffchange-inline">Moreover, if </ins>point <ins class="diffchange diffchange-inline">mutations or deletions were introduced </ins>into <ins class="diffchange diffchange-inline">such engineered cells</ins>, <ins class="diffchange diffchange-inline">it may </ins>cause serious problems to <ins class="diffchange diffchange-inline">the natural </ins>environment<ins class="diffchange diffchange-inline">, because they may cause the unexpected behavior (biohazard) against natural living systems</ins>. This is the big <ins class="diffchange diffchange-inline">issue in this field</ins>. <ins class="diffchange diffchange-inline">However, </ins>it is difficult to <ins class="diffchange diffchange-inline">invent </ins>an effective <ins class="diffchange diffchange-inline">approach to control the cell fate of the engineered bacteria</ins>. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Many </del>biologists <del class="diffchange diffchange-inline">try </del>to <del class="diffchange diffchange-inline">prevent them by using other functionally designed gene circuits, intending cell’s </del>population <del class="diffchange diffchange-inline">to be regulated </del>by negative feedback loops <del class="diffchange diffchange-inline">and then, as </del>a result, the <del class="diffchange diffchange-inline">cells cannot increase its number unlimitedly</del>. <del class="diffchange diffchange-inline">But those indirect systematic </del>regulations <del class="diffchange diffchange-inline">using other </del>gene <del class="diffchange diffchange-inline">circuits are difficult to tune up its parameters to cells and its living environment</del>. <del class="diffchange diffchange-inline">(Roughly to say</del>, <del class="diffchange diffchange-inline">it is very messy.) So </del>we <del class="diffchange diffchange-inline">hope </del>to <del class="diffchange diffchange-inline">make </del>a <del class="diffchange diffchange-inline">flexible method </del>to <del class="diffchange diffchange-inline">directly </del>control <del class="diffchange diffchange-inline">transformed cell’s behavior </del>without using <del class="diffchange diffchange-inline">those obstinate gene </del>circuits.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">For instance, several synthetic </ins>biologists <ins class="diffchange diffchange-inline">aimed </ins>to <ins class="diffchange diffchange-inline">regulate cell </ins>population by <ins class="diffchange diffchange-inline">introducing a </ins>negative feedback loops <ins class="diffchange diffchange-inline">or the other circuits in cells (REF). As </ins>a result, <ins class="diffchange diffchange-inline">they could control </ins>the <ins class="diffchange diffchange-inline">cell populations in some cases</ins>. <ins class="diffchange diffchange-inline">However, these </ins>regulations <ins class="diffchange diffchange-inline">can not precisely control the temporal </ins>gene <ins class="diffchange diffchange-inline">expression depending on cell division</ins>. <ins class="diffchange diffchange-inline">Thus</ins>, we <ins class="diffchange diffchange-inline">aimed </ins>to <ins class="diffchange diffchange-inline">design and construct </ins>a <ins class="diffchange diffchange-inline">system </ins>to control <ins class="diffchange diffchange-inline">the temporal gene expression depending on cell division </ins>without using <ins class="diffchange diffchange-inline">the other factors (e.g., small molecules such as Tetracycline or complicated genetic </ins>circuits <ins class="diffchange diffchange-inline">)</ins>. </div></td></tr>
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</table>ShoTakamori