Team:Kyoto/GSDD/Notebook/0824-0830

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0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

0824-0830 : Construction.PCR.


Tuesday, 25 August

To Do

  • PCR
  • Electrophoresis
  • Gel Extraction kit
    • 3-O-22
    • 1-O-2


Results

PCR
templateDNA sampleconc./(ng/ul)
3-O-2279.1
1-O-275.5

Kyoto 0825.png

3-O-22 PCR product (1,2)
1232bp
1-O-2 PCR product (3,4)
462bp
Extraction kit
sampleconc./(ng/ul)
(1)184.3
(1)288.9
(4)1181.3
(4)2126.2



Wednesday, 26 August

To Do

  • Restriction Enzyme Digestion
  • purification kit
  • ligation
  • Transformation
    • 3-O-22
    • 1-O-2

Results

PCR
templateDNA sampleconc./(ng/ul)
(1)222.3
(4)258.7
transformation

Kyoto 0826.jpg

  • colony
p-2(1)2
none
p-2(4)2
observed

We are going to try transformation of p【2】(4)② again.



Thursday, 27 August

To Do

  • (use p【2】(1)② after 0826 ligation)
    • Transformation
  • (use 【2】(1)① after 0825PCR, Gel extraction)
    • Restriction Enzyme Digestion
    • PCR purification kit
    • Ligation
    • Transformation


Results

Gel extraction kit
templateDNA sampleconc./(ng/ul)
1-(1)A38.1
1-(4)B21.4
transformation

Kyoto 0827.jpg

  • colony
p-2(1)2
observed
p-2(1)1A
none
p-2(1)1B
observed

We are going to make master plates of these sample.



Thursday, 27 August

To Do

  • 2(4)1(0825PCR)
    • Restriction Enzyme Digestion
    • PCR purification kit
    • ligation
    • transformation
  • p-2(1)1
  • p-2(1)2
  • p-2(4)2

(0825PCRed)

    • Make master plates.

Results

PCR purificaiton kit
sampleconc./(ng/ul)
(4)133.7
transformation

No colony was observed.

We are going to try transformation of p【2】(4)① again.