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Team:Kyoto/GSDD/Results - Revision history
2024-03-28T16:53:02Z
Revision history for this page on the wiki
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TADA at 02:55, 22 October 2009
2009-10-22T02:55:08Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#[[Team:Kyoto/GSDD|GSDD]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#[[Team:Kyoto/GSDD|GSDD]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Reslts & Discussion</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Reslts & Discussion</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Kyoto/topicpath}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div><!-- /#topicpath --></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div><!-- /#topicpath --></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Kyoto/Leftnavi}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Kyoto/Leftnavi}}</div></td></tr>
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TADA
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=166741&oldid=prev
Mako: /* Result-4 Analysis of the constructed TIMER vector in yeast */
2009-10-22T02:54:23Z
<p><span class="autocomment">Result-4 Analysis of the constructed TIMER vector in yeast</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:54, 22 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-4 Analysis of the constructed TIMER vector in yeast===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-4 Analysis of the constructed TIMER vector in yeast===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">同様</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">No data.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Discussion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Discussion==</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=166546&oldid=prev
Mako: /* Result of subgoal-3 Construction of the constitutive LacI expression vector */
2009-10-22T02:50:24Z
<p><span class="autocomment">Result of subgoal-3 Construction of the constitutive LacI expression vector</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:50, 22 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result of subgoal-3 Construction of the constitutive LacI expression vector===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result of subgoal-3 Construction of the constitutive LacI expression vector===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">同様</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''lacI generator for yeast (BBa_K210005)'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:32.png]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-4 Analysis of the constructed TIMER vector in yeast===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-4 Analysis of the constructed TIMER vector in yeast===</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=166502&oldid=prev
Mako: /* Products lengh check */
2009-10-22T02:49:41Z
<p><span class="autocomment">Products lengh check</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Length_check_blunt_end_clonig_by_Stu1_(BBa_K210014).png]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Length_check_blunt_end_clonig_by_Stu1_(BBa_K210014).png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Products lengh check===</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''lacI generator for yeast (BBa_K210005)'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts sequence===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts sequence===</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=162170&oldid=prev
ShoTakamori at 00:48, 22 October 2009
2009-10-22T00:48:08Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====1) Experimental conditions in MPR====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====1) Experimental conditions in MPR====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:figure(reaction2).png|<del class="diffchange diffchange-inline">300px</del>|<del class="diffchange diffchange-inline">right</del>|Fig.1]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:figure(reaction2).png|<ins class="diffchange diffchange-inline">220px</ins>|<ins class="diffchange diffchange-inline">center</ins>|Fig.1]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:figure(MPR1).png|<del class="diffchange diffchange-inline">250px</del>|<del class="diffchange diffchange-inline">right</del>|Fig.1]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:figure(MPR1).png|<ins class="diffchange diffchange-inline">420px</ins>|<ins class="diffchange diffchange-inline">center</ins>|Fig.1]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:figure(MPR2).png|<del class="diffchange diffchange-inline">600px</del>|<del class="diffchange diffchange-inline">right</del>|Fig.2]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:figure(MPR2).png|<ins class="diffchange diffchange-inline">420px</ins>|<ins class="diffchange diffchange-inline">center</ins>|Fig.2]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We tried various conditions in the temperature X. The result indicated that the temperature during MPR influences the length of the acquired sequence. At last, we programmed 72 o C of the temperature X. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We tried various conditions in the temperature X. The result indicated that the temperature during MPR influences the length of the acquired sequence. At last, we programmed 72 o C of the temperature X. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fig shows the repetitive sequence that we used for designing parts. As shown in Fig. 4, we succeeded in constructing the polymerized microgenes with different number of LacI binding sites.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fig shows the repetitive sequence that we used for designing parts. As shown in Fig. 4, we succeeded in constructing the polymerized microgenes with different number of LacI binding sites.</div></td></tr>
</table>
ShoTakamori
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=160290&oldid=prev
Mako: /* Result-3 Construction of the constitutive LacI expression vector */
2009-10-21T23:48:14Z
<p><span class="autocomment">Result-3 Construction of the constitutive LacI expression vector</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===Result-3 Construction of the constitutive LacI expression vector===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===Result <ins class="diffchange diffchange-inline">of subgoal</ins>-3 Construction of the constitutive LacI expression vector===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>同様</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>同様</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=160279&oldid=prev
Mako: /* Result-2 Construction of Timer vector that contains LacI generator and TIMER */
2009-10-21T23:47:49Z
<p><span class="autocomment">Result-2 Construction of Timer vector that contains LacI generator and TIMER</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===Result-2 Construction of Timer vector that contains LacI generator and TIMER===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===Result <ins class="diffchange diffchange-inline">of subgoal</ins>-2 Construction of Timer vector that contains LacI generator and TIMER===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Timer vector #1600 for measurement (BBa_K210008)'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Timer vector #1600 for measurement (BBa_K210008)'''</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=160219&oldid=prev
Mako: /* Result-2 Construction of Timer vector that contains LacI generator and TIMER */
2009-10-21T23:45:56Z
<p><span class="autocomment">Result-2 Construction of Timer vector that contains LacI generator and TIMER</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-2 Construction of Timer vector that contains LacI generator and TIMER===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-2 Construction of Timer vector that contains LacI generator and TIMER===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">ここにTIMERベクタのコンストラクションスキームなど入れる</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''Timer vector #1600 for measurement (BBa_K210008)'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:Timer_vector_check.png]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-3 Construction of the constitutive LacI expression vector===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Result-3 Construction of the constitutive LacI expression vector===</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=160207&oldid=prev
Mako: /* Products lengh check */
2009-10-21T23:45:38Z
<p><span class="autocomment">Products lengh check</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:45, 21 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''lacI generator for yeast (BBa_K210005)'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''lacI generator for yeast (BBa_K210005)'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Timer vector #1600 for measurement (BBa_K210008)'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Timer_vector_check.png]]</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts sequence===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts sequence===</div></td></tr>
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Mako
http://2009.igem.org/wiki/index.php?title=Team:Kyoto/GSDD/Results&diff=160193&oldid=prev
Mako: /* 2) Designing the repetitive part of LacI binding site */
2009-10-21T23:45:18Z
<p><span class="autocomment">2) Designing the repetitive part of LacI binding site</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:45, 21 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The repetitive part was inserted into PSB1A2 which has blunt end. In this process, we phosphorylated PSB1A2, and dephosphorylated the repetetitive. We purified them before ligation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The repetitive part was inserted into PSB1A2 which has blunt end. In this process, we phosphorylated PSB1A2, and dephosphorylated the repetetitive. We purified them before ligation.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> In conclusion, we successfully designed and constructed the repetitive sequences with the multiple protein binding sites by MPR. This is the simple method to construct any repetitive DNA sequence. Thus, it might be applicable to synthesize other repetitive sequences with different lengths in a tailor-made manner.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> In conclusion, we successfully designed and constructed the repetitive sequences with the multiple protein binding sites by MPR. This is the simple method to construct any repetitive DNA sequence. Thus, it might be applicable to synthesize other repetitive sequences with different lengths in a tailor-made manner.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''blunt end clonig by Stu1 (BBa_K210014)'''</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Products lengh check===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Products lengh check===</div></td></tr>
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Mako