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Team:LCG-UNAM-Mexico/Resources/Components
From 2009.igem.org
Biobricks used from the Registry of Standard Biological Parts
BBa_R1062
[http://partsregistry.org/Part:BBa_R1062 Promoter, Standard (luxR and HSL regulated -- lux pR)]
Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Drew Endy
Group: Registry
[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].
Promoter activated by LuxR in concert with HSL
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
BBa_C0261
[http://partsregistry.org/Part:BBa_C0261 AHL-making Enzyme, luxI (+RBS)]
