Team:LCG-UNAM-Mexico/Resources/Strains

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== '''Strains''' ==
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== '''E. coli Strains''' ==
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C-1a
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===Strains handed by Giuseppe Bertani===
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C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced.
   
   
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C-117.- Lysogenic for P2
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C-1906.- Lysogenic for P4.
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===Strains handed by Richard Calendar===
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C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/)
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The abbreviation "imp" means "improved plaques."
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C-520 F^+ /supD60/, used for growth and plaque assay of P2.
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C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay
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for P4. The symbol "lg" denotes large plaques.
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C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/)
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This strain is used to get the best P4 plaques. The symbol del1 denotes a
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deletion of the /old /gene, which interferes with the growth of phage
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lambda and kills /recB-/ bacteria.
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==Strain variants obtained in our lab==
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C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent [[Team:LCG-UNAM-Mexico/Journals/Nando's#September 9, 2009|stock of P4 sid1]]
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C-117
 

Revision as of 23:52, 20 October 2009


E. coli Strains

Strains handed by Giuseppe Bertani

C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced.

C-117.- Lysogenic for P2

C-1906.- Lysogenic for P4.

Strains handed by Richard Calendar

C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/) The abbreviation "imp" means "improved plaques."

C-520 F^+ /supD60/, used for growth and plaque assay of P2.

C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques.

C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/) This strain is used to get the best P4 plaques. The symbol del1 denotes a deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria.

Strain variants obtained in our lab

C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent stock of P4 sid1



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