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Team:LCG-UNAM-Mexico/Resources/Strains
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===Strains handed by Giuseppe Bertani=== | ===Strains handed by Giuseppe Bertani=== | ||
| - | C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced. | + | *C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced. |
| - | C-117.- Lysogenic for P2 | + | *C-117.- Lysogenic for P2 |
| - | C-1906.- Lysogenic for P4. | + | *C-1906.- Lysogenic for P4. |
===Strains handed by Richard Calendar=== | ===Strains handed by Richard Calendar=== | ||
| - | C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/) | + | *C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/). The abbreviation "imp" means "improved plaques." |
| - | The abbreviation "imp" means "improved plaques." | + | |
| - | C-520 F^+ /supD60/, used for growth and plaque assay of P2. | + | *C-520 F^+ /supD60/, used for growth and plaque assay of P2. |
| - | C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay | + | *C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques. |
| - | for P4. The symbol "lg" denotes large plaques. | + | |
| - | C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/) | + | *C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/). This strain is used to get the best P4 plaques. The symbol del1 denotes a |
| - | This strain is used to get the best P4 plaques. The symbol del1 denotes a | + | deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria. |
| - | deletion of the /old /gene, which interferes with the growth of phage | + | |
| - | lambda and kills /recB-/ bacteria. | + | |
===Strain variants obtained in our lab=== | ===Strain variants obtained in our lab=== | ||
| - | C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent [[Team:LCG-UNAM-Mexico:Journals:Nando's#September 19, 2009|stock of P4 sid1]] | + | *C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent [[Team:LCG-UNAM-Mexico:Journals:Nando's#September 19, 2009|stock of P4 sid1]] |
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| + | ===Expression Strains=== | ||
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| + | **BL21(DE3)pLysS from novagen: widely used for protein expression deficient in lon and ompT proteases. It has a chromosomal copy of the T7 RNA polymerase and T7 lysozyme for suppression o basal expression of T7 RNA polymerase and avoid as much as possible cell growth and viability affection. | ||
===Yeast strains=== | ===Yeast strains=== | ||
| - | TERRIBLY UNCOUNTABLE ANTIBIOTIC | + | TERRIBLY, UNCOUNTABLE, PERSISTENT and ANTIBIOTIC RESISTANT!!! |
Latest revision as of 03:19, 22 October 2009