Team:LCG-UNAM-Mexico/Resources/Strains

From 2009.igem.org

Revision as of 00:25, 22 October 2009 by Jurquiza (Talk | contribs)


E. coli Strains

Strains handed by Giuseppe Bertani

  • C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced.
  • C-117.- Lysogenic for P2
  • C-1906.- Lysogenic for P4.

Strains handed by Richard Calendar

  • C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/). The abbreviation "imp" means "improved plaques."
  • C-520 F^+ /supD60/, used for growth and plaque assay of P2.
  • C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques.
  • C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/). This strain is used to get the best P4 plaques. The symbol del1 denotes a

deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria.

Strain variants obtained in our lab

  • C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent stock of P4 sid1

Expression Strains

    • BL21(DE3)pLysS: widely used for protein expression deficient in lon and ompT proteases. It has a chromosomal copy of the T7 RNA polymerase and T7 lysozyme for suppression o basal expression of T7 RNA polymerase and avoid as much as possible

cell growth and viability affection.

Yeast strains

TERRIBLY, UNCOUNTABLE, PERSISTENT and ANTIBIOTIC RESISTANT!!!



Locations of visitors to this page