Team:MIT/Projects

From 2009.igem.org

Bilibuddies project.png

Home | Projects | Protocols | Parts for Registry | References


Project 1: Metabolic Engineering of PCB Synthesis in Yeast


PCB factory.jpg


Brief Description


Phycocyanobilin (PCB) is a chromophore necessary for PhyB-PIF3 based synthetic devices. Our goal is to engineer a yeast strain capable of synthesizing PCB, so that no exogenous PCB would be needed for PhyB-PIF3 to function in yeast. We thus cloned the genes encoding enzymes in the PCB biosynthetic pathway for expression in yeast
Details about Project 1


Project 2: Rapid & Reversible Protein Localization using PhyB-PIF3 System


Localization system.jpg

Brief Description


Our goal is to engineer a system that adopts the PhyB-PIF3 switch to control protein localization within the cell. In our design, either PhyB or PIF3 is constitutively anchored to one of the four target locations. The other will then be bound to our protein of interest and diffuse within the cell. Here, we use CFP and YFP to track the location of PhyB and PIF3, respectively. Reversable, light-dependent association of PhyB and PIF3 can then be monitored by fluorescence microscopy.
Details about Project 2