Team:Minnesota/Modeling

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!align="center"|[[Team:Minnesota|<font color="gold">Home</font>]]
!align="center"|[[Team:Minnesota|<font color="gold">Home</font>]]
!align="center"|[[Team:Minnesota/Team|<font color="gold">The Team</font>]]
!align="center"|[[Team:Minnesota/Team|<font color="gold">The Team</font>]]
!align="center"|[[Team:Minnesota/Project|<font color="gold">The Project</font>]]
!align="center"|[[Team:Minnesota/Project|<font color="gold">The Project</font>]]
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!align="center"|[[Team:Minnesota/Parts|<font color="gold">Parts Submitted to the Registry</font>]]
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!align="center"|[[Team:Minnesota/Designer|<font color="gold">SynBioSS Designer</font>]]
!align="center"|[[Team:Minnesota/Modeling|<font color="gold">Modeling</font>]]
!align="center"|[[Team:Minnesota/Modeling|<font color="gold">Modeling</font>]]
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!align="center"|[[Team:Minnesota/Notebook|<font color="gold">Notebook</font>]]
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!align="center"|[[Team:Minnesota/Notebook|<font color="gold">Experimental</font>]]
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!align="center"|[[Team:Minnesota/Parts Characterization|<font color="gold">Competition Requirements</font>]]
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[[Image:ModelingLab2.jpg|300px|right|The modelers in their natural environment.]]
[[Image:ModelingLab2.jpg|300px|right|The modelers in their natural environment.]]
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We want to improve the user interface for SynBioSS (see below), which was developed for last year's competition and also create mathematical models of our experimental results. Our computational output will guide future research and is the key to determining what is happening at the molecular level of our logical AND gate.  
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The primary goal of the modeling group is to create simulations of our experimental results. Our computational output will guide future research and helps us to understand what is happening at the molecular level of our logical AND gate.
 +
It is important to be able to observe the fidelity of the AND gate <i>in silico</i> as well as <i>in vivo</i>.  Using our in-house software suite, SynBioSS, we will create reaction networks describing the AND gate behavior with different constructs (e.g. TTN, TTL, TNN) and mutations. These reaction networks can then used to create the files necessary for simulation on the supercomputer. Certain kinetic constants in the reaction network can be adjusted to fit the simulation to experimental data. By observing which kinetic constants change with AND-gate construction or mutation we will then be able to understand some of the ways in which the AND-gate functions.
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<h2>Computational Tools</h2>
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<h2>Mathematical Modeling</h2>
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<h3>Overview</h3>
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[[Image:designgui.jpeg|300px|left|thumb|The MATLAB GUI displays all the reactions and chemical species in the network and allows the user to manipulate all aspects of the system such as reaction rates in order to better mimic experimental results.]]<p>In order to create a model, we must first use a program that will allow us to enter in the contents of the reactions network. The reaction network is formatted as .nc file, which is a special format called NetCDF that can interact with the group's supercomputer algorithms. The reaction network can be inputted directly into Linux as a text file and then converted into an .nc file. However, this is very tedious and error-prone process. In order to more quickly and accurately create the models, we use one of two programs: SynBioSS or a MATLAB GUI. Each of these programs allows us to graphically see what the model looks like and automatically converts the data describing the system into an .nc file. (See screenshots). <br> 
 +
Once the model is created, it is sent to a supercomputer where it is run using hybrid stochastic simulation algorithms created by the Kaznessis group. This algorithm takes the initial conditions of the model along with the reactions and kinetic constants and determines the state of the system over time. A stochastic model is used because the system that is described is very small. Cells are typically on the order of 1E-15 L. Because of this many molecular species exist in small quantities. When there are low numbers of molecules in a system, discrete changes in the number of molecules drastically change the reaction rates. Stochastic models use random number generators to describe the probabilistic nature of stochastic systems. <br>
 +
The results of the models are then retrieved from the supercomputer and analyzed using MATLAB. It is then possible to see if the model ran correctly and if it gave reasonable results. Depending on how the model compares to the experimental data, kinetic constants are changed and then the process of running a model begins again.<br>
 +
The accuracy of the model is determined by the difference in reporter gene expression between the model and the experiments. In order to make reasonable comparison the models must be rescaled, so that the model data matches with the experimental numerically. Basal expression models, which produce the maximum amount of reporter gene, are created for both the experimental and simulation data. This is done by removing the repressor molecules tetR2 and lacR4 from the system. Using this data, it is possible to rescale the model and the experimental data as a percentage of maximum expression. A least squares regression can then be done to determine the difference between the experimental results and the simulation. <br>
 +
Once the simulation is as close to the experimental results as possible a more accurate description of the AND-gate function can be constructed. This can be done by observing how changes in kinetic constants affect GFP expression. Or, using experimental data for mutant constructs, the simulations can be re-fit and the mutation's affect on the reaction network can be quantified. </p>
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[[Image:Synbiossflow.jpeg|250px|right|thumb|Over view of the SynBioSS workflow from Hill et al. (2008).]]
 
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[[Image:Synbiossdesigner.jpeg|250px|right|thumb|The interface for the SynBioSS designer where the user can input BioBricks to build a model.]]
 
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<h3> Synthetic Biology Software Suite (SynBioSS)</h3>
 
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The entire software suite can be found via the SynBioSS [http://synbioss.sourceforge.net home page]. The software suite is open source that runs on Windows, Macintosh and Linux. It does not require command-line programming and thus facilitates the modeling of biochemical reactions.
 
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<h3>SynBioSS Designer</h3>
 
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SynBioSS Designer is an application for the automatic generation of sets of biomolecular reactions. This software allows a user to input the molecular parts involved in gene expression and regulation (e.g. promoters, transcription factors, ribosomes, etc.) The software then generates complete networks of reactions that represent transcription, translation, regulation, induction and degradation of those parts. To facilitate the creation of detailed kinetic models of synthetic gene networks composed of BioBricks, we have adapted SynBioSS Designer to automatically generate a kinetic model from a construct composed entirely of BioBricks. A NetCDF or SBML file is generated for simulations.
 
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Our goal for this year's iGEM competition is to improve the user interface for SynBioSS by...
 
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<h3>SynBioSS Wiki</h3>
 
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The inaccessibility of requisite kinetic data complicates the generation of detailed mechanistic models. We address this barrier by creating a web accessible database curated by users in a “Wiki” format. SynBioSS Wiki is a significant extension of the open-source Mediawiki software. The Wiki stores reaction kinetic data in a formatted and searchable scheme with references to the relevant literature. This framework allows for the input of reactions whose rates are described either by elementary first & second order rate equations or any arbitrarily complex rate equation defined using MathML (e.g. Hill type reactions). Reactions may be searched via participating molecules which may be proteins, DNA sequences, small molecules, etc. Once located, reactions of interest (along with their associated kinetic data) may be collected. The completed model may be exported in SBML format for additional editing or simulation.
 
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It is also through the SynBioSS Wiki databases that SynBioSS Designer can access and proliferate kinetic information related to the simulation of BioBricks, thus extending the utility of the database for the benefit of the greater modeling community. To jumpstart the process, we have entered the known biomolecular interactions in the expression and regulation of well-studied operons, such as the lactose, the tetracycline and the arabinose operons.
 
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<h2>Mathematical Modeling</h2>
 
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[[Image:designgui.jpeg|300px|left|thumb|The MATLAB GUI displays all the reactions and chemical species in the network and allows the user to manipulate all aspects of the system such as reaction rates in order to better mimic experimental results.]]
 
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It is important to be able to observe the fidelity of the AND gate <i>in silico</i> as well as <i>in vivo</i>.  Using our in-house software suite, SynBioSS, we used networks of sixty or more reactions to model the AND gate with different constructs and mutations. Similar to the experimental procedures, we quantified the fidelity of our AND gate with GFP.  We also used a MATLAB Graphical User Interface (GUI) to analyze our experimental data. We used our empirical data from the wet lab to perfect our model.
 
<h2>Some Basic Modeling</h2>
<h2>Some Basic Modeling</h2>
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[[Image:reactionlist.jpg|400px|right|The reaction list in the MATLAB GUI with reactions 3 and 54 highlighted.]]
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[[Image:reactionlist.jpg|200px|right|The reaction list in the MATLAB GUI with reactions 3 and 54 highlighted.]]
We are working with four different combinations of <i>lac</i> and<i>tet</i> (L and T, respectively) operators in our promoter design project. These constructs are: T--, TT-, TTL and -T-. Here, we will demonstrate a little basic modeling we have done of the TTL construct using SynBioSS MATLAB and a design GUI.  
We are working with four different combinations of <i>lac</i> and<i>tet</i> (L and T, respectively) operators in our promoter design project. These constructs are: T--, TT-, TTL and -T-. Here, we will demonstrate a little basic modeling we have done of the TTL construct using SynBioSS MATLAB and a design GUI.  
-
On the SynBioSS wiki, the TTL model is available for download in a .nc format. Upon opening this with the MATLAB GUI, the list of 65 reactions that constitute the repression and induction of transcription. In this model, there are two <i>tet</i> operators TO1 and TO2 and one <i>lac</i> operator LO1. The reactions included in this list account for the repression and induction of transcription in the absence and presence of aTc and IPTG, respectively. In this simple model, we examined reactions 3 and 54, which involve the <i>tet</i> operators being bound by the repressor. (These reactions are shown in the GUI on the left.) We varied the reactions constants from the original 10<sup>8</sup> as low as 0 and as high as 10<sup>9</sup> to see if the fidelity of the AND gate could depend on the reaction rates for repression. We quantified our results by counting molecules of GFP. This not only gave us a clearer idea of what was happening at the molecular level at this promoter site, but also served as a preparation for matching the experimental results computationally.  
+
On the SynBioSS wiki, the TTL model is available for download in a .nc format. Upon opening this with the MATLAB GUI, the list of 65 reactions that constitute the repression and induction of transcription. In this model, there are two <i>tet</i> operators TO1 and TO2 and one <i>lac</i> operator LO1. The reactions included in this list account for the repression and induction of transcription in the absence and presence of aTc and IPTG, respectively. In this simple model, we examined reactions 3 and 54, which involve the <i>tet</i> operators being bound by the repressor. (These reactions are shown in the GUI on the left.) We varied the reactions constants from the original 10<sup>8</sup> as low as 0 and as high as 10<sup>9</sup> to see if the fidelity of the AND gate could depend on the reaction rates for repression. We quantified our results by counting molecules of GFP. This not only gave us a clearer idea of what was happening at the molecular level at this promoter site, but also served as a preparation for matching the experimental results computationally.
-
The initial results of our modification of the reaction constants were graphs that showed a steady increase in GFP with time and aTc concentration that eventually plateaued and held GFP constant around 12 molecules. At the plateau as you can see on the graph on the below top left, the gfp oscilated with time despite aTc concentration because the cells were splitting every hour. To smooth out this oscillation, we chose timepoints when the cells were not doubling and graphed gfp again with respect to time and aTc concentration. This graph is shown at the below top right.
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<h2>Calendar</h2>
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{|  align="center"
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These similar graphs below with k=10<sup>9</sup> (bottom left) and 10<sup>6</sup> (bottom right) reinforce the idea of biological complexity. The fidelity of the AND gate does not appear to be dependent on these two reactions alone.
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{| style="color:gold;background-color:#800000;" cellpadding="3" cellspacing="1" border="10" bordercolor="#00CCFF" width="62%" align="center"
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!align="center"|{{#calendar: title=Minnesota |year=2009 | month=06}}
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Of the numerous other reactions included in this model, four others involved the binding of one or two molecules of aTc to the <i>tet</i> repressor and thereby inducing transcription. So we decided to investigate the effects of different reaction constants for these reactions on GFP production.
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!align="center"|{{#calendar: title=Minnesota |year=2009 | month=07}}
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!align="center"|{{#calendar: title=Minnesota |year=2009 | month=08}}
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!align="center"|{{#calendar: title=Minnesota |year=2009 | month=09}}
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[[Image:K108graph.jpg|left|350px|Graph of gfp with respect to time and aTc concentration with k=10^8 as in the original model]]
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<h2>Results</h2>
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[[Image:K108graphfinite.jpg|left|350px|Graph of gfp at certain timepoints for k=10^8]]
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Place results of models here.
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[[Image:K109graphfinite.jpg|left|350px|Graph of gfp at certain timepoints for k=10^9]]
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[[Image:K106graphfinite.jpg|left|350px|Graph of grp at certain timepoints for k=10^6]]
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Latest revision as of 19:22, 7 August 2009


Mnlogo.jpg
Home The Team The Project SynBioSS Designer Modeling Experimental Competition Requirements


Contents

Our Modeling Goals

The modelers in their natural environment.

The primary goal of the modeling group is to create simulations of our experimental results. Our computational output will guide future research and helps us to understand what is happening at the molecular level of our logical AND gate. It is important to be able to observe the fidelity of the AND gate in silico as well as in vivo. Using our in-house software suite, SynBioSS, we will create reaction networks describing the AND gate behavior with different constructs (e.g. TTN, TTL, TNN) and mutations. These reaction networks can then used to create the files necessary for simulation on the supercomputer. Certain kinetic constants in the reaction network can be adjusted to fit the simulation to experimental data. By observing which kinetic constants change with AND-gate construction or mutation we will then be able to understand some of the ways in which the AND-gate functions.

Mathematical Modeling

Overview

The MATLAB GUI displays all the reactions and chemical species in the network and allows the user to manipulate all aspects of the system such as reaction rates in order to better mimic experimental results.

In order to create a model, we must first use a program that will allow us to enter in the contents of the reactions network. The reaction network is formatted as .nc file, which is a special format called NetCDF that can interact with the group's supercomputer algorithms. The reaction network can be inputted directly into Linux as a text file and then converted into an .nc file. However, this is very tedious and error-prone process. In order to more quickly and accurately create the models, we use one of two programs: SynBioSS or a MATLAB GUI. Each of these programs allows us to graphically see what the model looks like and automatically converts the data describing the system into an .nc file. (See screenshots).

Once the model is created, it is sent to a supercomputer where it is run using hybrid stochastic simulation algorithms created by the Kaznessis group. This algorithm takes the initial conditions of the model along with the reactions and kinetic constants and determines the state of the system over time. A stochastic model is used because the system that is described is very small. Cells are typically on the order of 1E-15 L. Because of this many molecular species exist in small quantities. When there are low numbers of molecules in a system, discrete changes in the number of molecules drastically change the reaction rates. Stochastic models use random number generators to describe the probabilistic nature of stochastic systems.
The results of the models are then retrieved from the supercomputer and analyzed using MATLAB. It is then possible to see if the model ran correctly and if it gave reasonable results. Depending on how the model compares to the experimental data, kinetic constants are changed and then the process of running a model begins again.
The accuracy of the model is determined by the difference in reporter gene expression between the model and the experiments. In order to make reasonable comparison the models must be rescaled, so that the model data matches with the experimental numerically. Basal expression models, which produce the maximum amount of reporter gene, are created for both the experimental and simulation data. This is done by removing the repressor molecules tetR2 and lacR4 from the system. Using this data, it is possible to rescale the model and the experimental data as a percentage of maximum expression. A least squares regression can then be done to determine the difference between the experimental results and the simulation.

Once the simulation is as close to the experimental results as possible a more accurate description of the AND-gate function can be constructed. This can be done by observing how changes in kinetic constants affect GFP expression. Or, using experimental data for mutant constructs, the simulations can be re-fit and the mutation's affect on the reaction network can be quantified.




Some Basic Modeling

The reaction list in the MATLAB GUI with reactions 3 and 54 highlighted.

We are working with four different combinations of lac andtet (L and T, respectively) operators in our promoter design project. These constructs are: T--, TT-, TTL and -T-. Here, we will demonstrate a little basic modeling we have done of the TTL construct using SynBioSS MATLAB and a design GUI.

On the SynBioSS wiki, the TTL model is available for download in a .nc format. Upon opening this with the MATLAB GUI, the list of 65 reactions that constitute the repression and induction of transcription. In this model, there are two tet operators TO1 and TO2 and one lac operator LO1. The reactions included in this list account for the repression and induction of transcription in the absence and presence of aTc and IPTG, respectively. In this simple model, we examined reactions 3 and 54, which involve the tet operators being bound by the repressor. (These reactions are shown in the GUI on the left.) We varied the reactions constants from the original 108 as low as 0 and as high as 109 to see if the fidelity of the AND gate could depend on the reaction rates for repression. We quantified our results by counting molecules of GFP. This not only gave us a clearer idea of what was happening at the molecular level at this promoter site, but also served as a preparation for matching the experimental results computationally.

Calendar

June
MTWTFSS
[http://2009.igem.org/Minnesota/1_June_2009 1] [http://2009.igem.org/Minnesota/2_June_2009 2] [http://2009.igem.org/Minnesota/3_June_2009 3] [http://2009.igem.org/Minnesota/4_June_2009 4] [http://2009.igem.org/Minnesota/5_June_2009 5] [http://2009.igem.org/wiki/index.php?title=Minnesota/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Minnesota/7_June_2009&action=edit 7]
[http://2009.igem.org/Minnesota/8_June_2009 8] [http://2009.igem.org/Minnesota/9_June_2009 9] [http://2009.igem.org/Minnesota/10_June_2009 10] [http://2009.igem.org/Minnesota/11_June_2009 11] [http://2009.igem.org/Minnesota/12_June_2009 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Minnesota/14_June_2009&action=edit 14]
[http://2009.igem.org/Minnesota/15_June_2009 15] [http://2009.igem.org/Minnesota/16_June_2009 16] [http://2009.igem.org/Minnesota/17_June_2009 17] [http://2009.igem.org/Minnesota/18_June_2009 18] [http://2009.igem.org/Minnesota/19_June_2009 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Minnesota/21_June_2009&action=edit 21]
[http://2009.igem.org/Minnesota/22_June_2009 22] [http://2009.igem.org/Minnesota/23_June_2009 23] [http://2009.igem.org/Minnesota/24_June_2009 24] [http://2009.igem.org/Minnesota/25_June_2009 25] [http://2009.igem.org/Minnesota/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Minnesota/28_June_2009&action=edit 28]
[http://2009.igem.org/Minnesota/29_June_2009 29] [http://2009.igem.org/Minnesota/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Minnesota/1_July_2009 1] [http://2009.igem.org/Minnesota/2_July_2009 2] [http://2009.igem.org/Minnesota/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Minnesota/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_July_2009&action=edit 5]
[http://2009.igem.org/Minnesota/6_July_2009 6] [http://2009.igem.org/Minnesota/7_July_2009 7] [http://2009.igem.org/Minnesota/8_July_2009 8] [http://2009.igem.org/Minnesota/9_July_2009 9] [http://2009.igem.org/Minnesota/10_July_2009 10] [http://2009.igem.org/Minnesota/11_July_2009 11] [http://2009.igem.org/Minnesota/12_July_2009 12]
[http://2009.igem.org/Minnesota/13_July_2009 13] [http://2009.igem.org/Minnesota/14_July_2009 14] [http://2009.igem.org/Minnesota/15_July_2009 15] [http://2009.igem.org/Minnesota/16_July_2009 16] [http://2009.igem.org/Minnesota/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_July_2009&action=edit 19]
[http://2009.igem.org/Minnesota/20_July_2009 20] [http://2009.igem.org/Minnesota/21_July_2009 21] [http://2009.igem.org/Minnesota/22_July_2009 22] [http://2009.igem.org/Minnesota/23_July_2009 23] [http://2009.igem.org/Minnesota/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_July_2009&action=edit 26]
[http://2009.igem.org/Minnesota/27_July_2009 27] [http://2009.igem.org/Minnesota/28_July_2009 28] [http://2009.igem.org/Minnesota/29_July_2009 29] [http://2009.igem.org/Minnesota/30_July_2009 30] [http://2009.igem.org/Minnesota/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Minnesota/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Minnesota/2_August_2009&action=edit 2]
[http://2009.igem.org/Minnesota/3_August_2009 3] [http://2009.igem.org/Minnesota/4_August_2009 4] [http://2009.igem.org/Minnesota/5_August_2009 5] [http://2009.igem.org/Minnesota/6_August_2009 6] [http://2009.igem.org/Minnesota/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=Minnesota/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Minnesota/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Minnesota/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Minnesota/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Minnesota/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Minnesota/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Minnesota/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Minnesota/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Minnesota/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Minnesota/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Minnesota/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Minnesota/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Minnesota/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Minnesota/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Minnesota/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Minnesota/31_August_2009&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/wiki/index.php?title=Minnesota/1_September_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Minnesota/2_September_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Minnesota/3_September_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Minnesota/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Minnesota/6_September_2009&action=edit 6]
[http://2009.igem.org/wiki/index.php?title=Minnesota/7_September_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Minnesota/8_September_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Minnesota/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Minnesota/10_September_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Minnesota/11_September_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_September_2009&action=edit 13]
[http://2009.igem.org/wiki/index.php?title=Minnesota/14_September_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Minnesota/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Minnesota/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Minnesota/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=Minnesota/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Minnesota/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Minnesota/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Minnesota/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=Minnesota/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Minnesota/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Minnesota/30_September_2009&action=edit 30]

Results

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