Team:Minnesota/Parts Characterization
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+ | <h1>Parts Characterization</h1> | ||
+ | |||
+ | We chose five promoters that were included in the 2009 iGEM Kit to characterize. Using part [http://partsregistry.org/wiki/index.php/Part:BBa_F2620 BBa_F2620], which was characterized by a group from MIT in 2004, as a template, we characterized the following parts: | ||
+ | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Part</th><th>Description</th><th>Regulators</th><th>People</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[http://partsregistry.org/Part:BBa_I14032 I14032]</td><td>Constitutive promoter classified as repressible</td><td>IPTG</td><td>Princeton 2004</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[http://partsregistry.org/Part:BBa_J13002 J13002]</td><td>Two TetR binding sites and RBS</td><td>aTc</td><td>UT Austin 2005</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[http://partsregistry.org/Part:BBa_I14015 I14015]</td><td>LasR, 3OC12HSL aTc regulated promoter</td><td>LasR, 3C12HSL, aTc</td><td>Princeton 2004</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[http://partsregistry.org/Part:BBa_K091101 K091101]</td><td>TTL AND gate</td><td>IPTG, aTc</td><td>Davidson Missouri-Western 2008</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[http://partsregistry.org/Part:BBa_R0011 R0011]</td><td>Inverting regulatory region controlled by LacI</td><td>IPTG</td><td>Registry</td> | ||
+ | </tr> | ||
+ | </table> |
Revision as of 17:17, 13 July 2009
Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Parts Characterization | Notebook |
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Parts Characterization
We chose five promoters that were included in the 2009 iGEM Kit to characterize. Using part BBa_F2620, which was characterized by a group from MIT in 2004, as a template, we characterized the following parts:
Part | Description | Regulators | People |
---|---|---|---|
I14032 | Constitutive promoter classified as repressible | IPTG | Princeton 2004 |
J13002 | Two TetR binding sites and RBS | aTc | UT Austin 2005 |
I14015 | LasR, 3OC12HSL aTc regulated promoter | LasR, 3C12HSL, aTc | Princeton 2004 |
K091101 | TTL AND gate | IPTG, aTc | Davidson Missouri-Western 2008 |
R0011 | Inverting regulatory region controlled by LacI | IPTG | Registry |