Team:Minnesota/Parts Characterization

From 2009.igem.org

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<h1>Parts Characterization</h1>
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We chose five promoters that were included in the 2009 iGEM Kit to characterize. Using part [http://partsregistry.org/wiki/index.php/Part:BBa_F2620 BBa_F2620], which was characterized by a group from MIT in 2004, as a template, we characterized the following parts:
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<th>Part</th><th>Description</th><th>Regulators</th><th>People</th>
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<td>[http://partsregistry.org/Part:BBa_I14032 I14032]</td><td>Constitutive promoter classified as repressible</td><td>IPTG</td><td>Princeton 2004</td>
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<td>[http://partsregistry.org/Part:BBa_J13002 J13002]</td><td>Two TetR binding sites and RBS</td><td>aTc</td><td>UT Austin 2005</td>
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<td>[http://partsregistry.org/Part:BBa_I14015 I14015]</td><td>LasR, 3OC12HSL aTc regulated promoter</td><td>LasR, 3C12HSL, aTc</td><td>Princeton 2004</td>
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<td>[http://partsregistry.org/Part:BBa_K091101 K091101]</td><td>TTL AND gate</td><td>IPTG, aTc</td><td>Davidson Missouri-Western 2008</td>
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<td>[http://partsregistry.org/Part:BBa_R0011 R0011]</td><td>Inverting regulatory region controlled by LacI</td><td>IPTG</td><td>Registry</td>
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Revision as of 17:17, 13 July 2009

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Parts Characterization

We chose five promoters that were included in the 2009 iGEM Kit to characterize. Using part BBa_F2620, which was characterized by a group from MIT in 2004, as a template, we characterized the following parts:

PartDescriptionRegulatorsPeople
I14032Constitutive promoter classified as repressibleIPTGPrinceton 2004
J13002Two TetR binding sites and RBSaTcUT Austin 2005
I14015LasR, 3OC12HSL aTc regulated promoterLasR, 3C12HSL, aTcPrinceton 2004
K091101TTL AND gateIPTG, aTcDavidson Missouri-Western 2008
R0011Inverting regulatory region controlled by LacIIPTGRegistry