Team:NCTU Formosa/Notebook/Calendar

From 2009.igem.org

July

7/15

It is the beginning today!!!!!!!!

7/17

Lesson:Parts, Devices and Biobricks.  In this lesson we learned about the principle of the construction of biobricks.

7/20~22

To present 2008 IGEM projects.

7/27~29

To present everyone’s primary idea.

August

8/03

A group duscussion of each one's personal idea.

8/05

To collate everyone’s advantages.

8/07

A group discussion.   We decided what kind of projects we're going to construct.

8/12

A group discussion.  We discussed about the principle and some details of the project. We also designed the first step of the experimence and the protocols.

8/17

Today we devided our team into 5 subgroups and started the experimence.   In each subgroup, we have to contribute a part of the whole project.

8/18

We transformed the bricks we need from the well.

8/21

we check DNA by agarose gel, SDS-PAGE.

8/22~23

To transfer E. coli to new LB broth, and incubate for 16hr, then Extract plasmid.

8/28

we design a logo of team NCTU_FORMOSA

September

9/1

The experiment is not so smoothly, we sink into infinite loop in the same experiments.

9/2

To digest the front part (8/23) and the behind part (8/26) of the biobrick, ligate, transform and run gel to check that if digestion is OK or not, then spread on the plate, and incubate for 20hr.

9/7

We use another enzyme to digest to double check if the plasmid is right or not

9/10

Today we have a meeting that we discuss how to solve the problems.

9/14~25

The plasmid's sequences are wrong and we repeat the digestion, ligation, and transform again.

9/16

BBa_K188343 is sequenced today.

9/28

Degenerated PCR, using BBa_K145279 as template

9/29

Degenerated PCR

October

10/2

Degenerated PCR. Restriction enzyme map.

10/7

Gel purification. To ligate gel purification products

10/11

to make restriction enzyme map. To select 14 plasmids

10/12

We had a team work to work on the wiki web. Flow cytometer.

10/14

Submitted the parts to be sequenced.

10/18

Updated the web of notebook and the project.   We also had a group discussion about our results and the data.

10/21

To discuss our final result.