Team:NTU-Singapore/Project/Wetlab

From 2009.igem.org

(Difference between revisions)
(Successful Constructs)
Line 35: Line 35:
# J23119-B0034-GFP-B0015 (''characterization construct'')
# J23119-B0034-GFP-B0015 (''characterization construct'')
# pLacI-B0034-CHE-B0015 (''test construct'')
# pLacI-B0034-CHE-B0015 (''test construct'')
-
# J23199-B0034-HO1-B0015-J23119-B0034-IFP1.4-B0015 (''test construct'')
+
# J23119-B0034-IFP-B0015-J23119-B0034-HO1-B0015 (''test construct'')

Revision as of 19:50, 21 October 2009

Conclusive Results


Successful Constructs

The following are the constructs that we have shown to be working.

  1. J23119-B0034-NorR-B0015 (system construct)
  2. J23119-B0034-GFP-B0015 (characterization construct)
  3. pLacI-B0034-CHE-B0015 (test construct)
  4. J23119-B0034-IFP-B0015-J23119-B0034-HO1-B0015 (test construct)


The first construct is a system construct, in that it was intended for our system to feature this as a functional part.

The second construct is a characterization construct to characterize the modular function of the J23119 promoter in the transcription of NorR.

The third construct is also a test construct to only test and characterize the enzymatic activity of CHE.

The fourth construct is a test construct to only test the reporting ability of IFP.





NTU@iGEMcc 2009. Some rights reserved.