Team:NYMU-Taipei/Experiments

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Revision as of 21:03, 21 October 2009 by Blackrabbit (Talk | contribs)

NYMU Wt--experi.png


Contents

Experimental Results

Protocols

Sequences, Primers

Sequences, primers and PCR results by subteam:

PCR results

Parts

K195100-K195199

Cellular Receptors: CD4

Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195100

CD4 gene
K195100

NYMU icon C.png

coding 1377 BBa_K195100 This part is human CD4 gene. The coding sequence CD4 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120. NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195101

K195101
CD4 gene
K195100

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1506 BBa_K195100 BBa_B0015 This part is composed of CD4 gene and terminator. The coding sequence CD4 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120.
BBa_K195102

K195102
Lpp-OmpA-linker
K103006

NYMU icon C.png
CD4 gene
K195100

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1969 BBa_K103006 BBa_K195100 BBa_B0015 This part is composed of Lpp-OmpA-linker, CD4 gene and terminator. Fusion CD4 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195103

K195103
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
CD4 gene
K195100

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1981 BBa_B0034 BBa_K103006 BBa_K195100 BBa_B0015 This part is composed of RBS, Lpp-OmpA-linker, CD4 gene and terminator. Fusion CD4 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195104

K195104
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
CD4 gene
K195100

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 2035 BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195100 BBa_B0015 This part is composed of pTet, RBS, Lpp-OmpA-linker, CD4 gene and terminator. Fusion CD4 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.

Cellular Receptors: CCR5

Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195105

CCR5 gene
K195105

NYMU icon C.png

coding 1059 BBa_K195105 This part is human CCR5 gene. The coding sequence CCR5 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120. NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195106

K195106
CCR5 gene
K195105

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1188 BBa_K195105 BBa_B0015 This part is composed of CCR5 gene and terminator.

The coding sequence CCR5 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120.

BBa_K195107

K195107
Lpp-OmpA-linker
K103006

NYMU icon C.png
CCR5 gene
K195105

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1651 BBa_K103006 BBa_K195105 BBa_B0015 This part is composed of Lpp-OmpA-linker, CCR5 gene and terminator. Fusion CCR5 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195108

K195108
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
CCR5 gene
K195105

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1663 BBa_B0034 BBa_K103006 BBa_K195105 BBa_B0015 This part is composed of RBS, Lpp-OmpA-linker, CCR5 gene and terminator. Fusion CCR5 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195109

K195109
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
CCR5 gene
K195105

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1717 BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195105 BBa_B0015 This part is composed of pTet, RBS, Lpp-OmpA-linker, CCR5 gene and terminator. Fusion CCR5 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.

K195200-K195299

Cellular Receptors: Integrin

Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195200

alpha-V integrin gene
K195200

NYMU icon C.png

coding 3039 BBa_K195200 This part is human alpha-V integrin gene. The coding sequence alpha-V integrin is a receptor on human cell, it may interact with capsid protein penton base of several virus. NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195201

K195201
alpha-V integrin gene
K195200

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 3168 BBa_K195200 BBa_B0015 This part is composed of alpha-V integrin gene and terminator. The coding sequence alpha-V integrin is a receptor on human cell, it may interact with capsid protein penton base of several virus.
BBa_K195202

K195202
Lpp-OmpA-linker
K103006

NYMU icon C.png
alpha-V integrin gene
K195200

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 3631 BBa_K103006 BBa_K195200 BBa_B0015 This part is composed of Lpp-OmpA-linker, alpha-V integrin gene and terminator. Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.
BBa_K195203

K195203
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
alpha-V integrin gene
K195200

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 3643 BBa_B0034 BBa_K103006 BBa_K195200 BBa_B0015 This part is composed of RBS, Lpp-OmpA-linker, alpha-V integrin gene and terminator. Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.
BBa_K195204

K195204
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
alpha-V integrin gene
K195200

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 3697 BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195200 BBa_B0015 This part is composed of pTet, RBS, Lpp-OmpA-linker, alpha-V integrin gene and terminator. Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.

Cellular Receptors: CXADR(CAR)

Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195205

CXADR gene
K195205

NYMU icon C.png

coding 1098 BBa_K195206 This part is human CXADR gene. The coding sequence CXADR receptor is a receptor on human cell, it may interact with capsid fiber protein of several virus. NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195206

K195206
CXADR gene
K195205

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1227 BBa_K195200 BBa_B0015 This part is composed of CXADR gene and terminator. The coding sequence CXADR receptor is a receptor on human cell, it may interact with capsid fiber protein of several virus.
BBa_K195207

K195207
Lpp-OmpA-linker
K103006

NYMU icon C.png
CXADR gene
K195205

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1690 BBa_K103006 BBa_K195200 BBa_B0015 This part is composed of Lpp-OmpA-linker, CXADR gene and terminator. Fusion CXADR gene and Lpp-ompA-Linker(BBa_K103006) together, so CXADR receptor can anchor on the outer membrane.
BBa_K195208

K195208
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
CXADR gene
K195205

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1702 BBa_B0034 BBa_K103006 BBa_K195200 BBa_B0015 This part is composed of RBS, Lpp-OmpA-linker, CXADR gene and terminator. Fusion CXADR gene and Lpp-ompA-Linker(BBa_K103006) together, so CXADR receptor can anchor on the outer membrane.
BBa_K195209

K195209
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
CXADR gene
K195205

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1756 BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195205 BBa_B0015 This part is composed of pTet, RBS, Lpp-OmpA-linker, CXADR gene and terminator. Fusion CXADR gene and Lpp-ompA-Linker(BBa_K103006) together, so CXADR receptor can anchor on the outer membrane.

K195300-K195399

Cellular Receptors: Sialic Acid

Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195300

neu gene operon
K195300

NYMU icon C.png

coding 5229 BBa_K195300 This part is composed of neuD,neuB,neuA,neuC,neuE(neu gene cluster). The coding sequence neu genes are operate in one operon in E.coli K1, which are coding for the enzymes that are involved in sialic acid synthesis. NCKU provided us the E.coli K1.
BBa_K195301

K195301
neu gene operon
K195300

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite BBa_K195300 BBa_B0015 This part is composed of neu gene operon and terminator.
BBa_K195302

K195302
RBS
B0034

NYMU icon R.png
neu gene operon
K195300

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator BBa_B0034 BBa_K195300 BBa_B0015 This part is composed of RBS,neu gene operon and terminator. The coding sequence aim at sialic acid synthesis.
BBa_K195303

K195303
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
neu gene operon
K195300

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator BBa_R0040 BBa_B0034 BBa_K195300 BBa_B0015 This part is composed of pTet, RBS, neu gene operon and terminator. The pTet promoter is strong enough to overexpress the neu gene operon.
BBa_K195304

sialyltransferase 0160
K195304

NYMU icon C.png

coding 2006 BBa_K195304 This part is the coding sequence of alpha-2,6-sialyltransferase. alpha-2,6-sialyltransferase can transfer polysaccharides onto the cell membrane to fuse with the membrane proteins, which then form glycoproteins.
BBa_K195305

K195305
sialyltransferase 0160
K195304

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite BBa_K195304 BBa_B0015 This part is composed of sialyltransferase 0160 and terminator.
BBa_K195306

K195306
RBS
B0034

NYMU icon R.png
sialyltransferase 0160
K195304

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator BBa_B0034 BBa_K195304 BBa_B0015 This part is composed of RBS,sialyltransferase 0160 and terminator.
BBa_K195307

K195307
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
sialyltransferase 0160
K195304

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator BBa_R0040 BBa_B0034 BBa_K195304 BBa_B0015 This part is composed of pTet, RBS, sialyltransferase 0160 and terminator. The pTet promoter is strong enough to overexpress the sialyltransferase 0160.

K195400-K195499

Cellular Receptors: Antibody

Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195400

F10scFv
K195400

NYMU icon C.png

Coding 840 K195400 single chain Fv (Antibody) that bind to InfluenzaF10scFv can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide. synthesis by Mr.gene
BBa_K195401

K195401
Lpp-OmpA-linker
K103006

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png

composite 1304 BBa_K103006 BBa_K195400 Fusion BBa_K195400 with BBa_K103006 Fusion our BBa_K195400 with BBa_K103006, so the F10scFv can be anchored on surface of E. coli. Scarless in-fusion by PCR
BBa_K195402

K195402
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png

generator 1332 BBa_B0034 BBa_K103006 BBa_K195400 This part is composed of RBS,Lpp-OmpA-linker and F10scFv The coding sequence can express F10scFv(BBa_K195400) on outer membrane by OmpA(BBa_K103006).
BBa_K195403

K195403
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png

generator 1384 BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195400 This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
BBa_K195404

K195404
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
Lpp-OmpA-linker
K103006

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 1531 BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195400 BBa_B0015 This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
BBa_K195410

C-IgAP
K195405

NYMU icon C.png

coding 1257 BBa_K195405 C-terminal of IgA protease The leader sequence of C-IgAP is cleaved at the membrane by a signal peptidase, releasing the mature polyprotein into the periplasm. When in the periplasm, the beta-domain of the protein inserts into the outer membrane to form a beta-barrel structure. After formation of the beta-barrel pore, the passenger domain is translocated to the cell surface through the pore. Once at the cell surface, several possibilities may occur. Neisseria gonorrhoeae FA1090(provide by Dr.Hsing-Ju Wu, Academia sinica/Institute of Biological Chemistry)
BBa_K195406

K195406
F10scFv
K195400

NYMU icon C.png
C-IgAP
K195406

NYMU icon C.png

composite 2097 BBa_K195400 BBa_K195406 This part is composed of F10scFv and C-IgAP scFv fusion with C-IgAP
BBa_K195407

K195407
PelB
J32015

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png

composite 906 BBa_J32015 BBa_K195400 This part is composed of PelB and F10scFv scFv fusion with PelB
BBa_K195408

K195408
PelB
J32015

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png
C-IgAP
K195406

NYMU icon C.png

composite 2163 BBa_J32015 BBa_K195400 BBa_K195406 This part is composed of PelB,F10scFv and C-IgAP
BBa_K195409

K195409
RBS
B0034

NYMU icon R.png
PelB
J32015

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png
C-IgAP
K195406

NYMU icon C.png

generator 2197 BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 This part is composed of RBS,PelB,F10scFv and C-IgAP The coding sequence express F10scFv on E.coli surface
BBa_K195412

K195410
RBS
B0034

NYMU icon R.png
PelB
J32015

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png
C-IgAP
K195406

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 2334 BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 BBa_B0015 This part is composed of RBS,PelB,F10scFv,C-IgAP and terminator The coding sequence express F10scFv on E.coli surface
BBa_K195411

K195411
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
PelB
J32015

NYMU icon C.png
F10scFv
K195400

NYMU icon C.png
C-IgAP
K195406

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 2396 BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 BBa_B0015 This part is composed of pTetR,RBS,PelB,F10scFv,C-IgAP and terminatorThe coding sequence express F10scFv on E.coli surface


K195600-K195699

Removal


Part Name Picture Part Type Part Length Subparts Short Description (max 60 chars) Long Description (what it is, what it does, how to use) Design Notes Source (where does it come from?)
BBa_K195600

K195600
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 431 BBa_K116602 BBa_B0015 This part is composed of CII and terminator. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. This part comes from IGEM_08 NYMU-Taipei.
BBa_K195601

K195601
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 449 BBa_B0034 BBa_K116602 BBa_B0015 This part is composed of RBS,CII and terminator. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
BBa_K195602

K195602
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 822 BBa_C0040 BBa_B0015 This part is composed of TetR and terminator. The coding sequence TetR could repress the regulatory promoter pTetR(BBa_R0040).
BBa_K195603

K195603
RBS
B0034

NYMU icon R.png
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 840 BBa_B0034 BBa_C0040 BBa_B0015 This part is composed of RBS,TetR and terminator. The coding sequence TetR could repress the regulatory promoter pTet(BBa_R0040).
BBa_K195604

K195604
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 585 BBa_C0074 BBa_B0015 This part is composed of PenI and terminator. The coding sequence PenI could repress regulatory promoter pPenI(BBa_R0074).
BBa_K195605

K195605
RBS
B0034

NYMU icon R.png
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 603 BBa_B0034 BBa_C0074 BBa_B0015 This part is composed of RBS,PenI and terminator. The coding sequence PenI could repress regulatory promoter pPenI(BBa_R0074).
BBa_K195606

K195606
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 511 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 This part is composed of pTetR, RBS,CII and terminator. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).
BBa_K195607

K195607
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 534 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 This part is composed of pPenI, RBS,CII and terminator. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).
BBa_K195608

K195608
pLacI
R0010

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 657 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 This part is composed of pLacI, RBS,CII and terminator. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
BBa_K195609

K195609
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 931 BBa_K116603 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pRE and GFP generator. The promoter pRE could be activated by BBa_K116602,the activator CII.
BBa_K195610

K195610
pTet
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 937 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pTet and GFP generator. The promoter pTet could be repressed by BBa_C0040,the repressor TetR.
BBa_K195611

K195611
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 960 BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pPenI and GFP generator. The promoter pPenI could be repressed by BBa_C0074,the repressor PenI.
BBa_K195612

K195612
pLacI
R0010

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 1083 BBa_R0010 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pLacI and GFP generator. The pLacI is repressed by LacI, which could be inhibited by IPTG.
BBa_K195613

K195613
pCI
R1051

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 932 BBa_R1051 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pCI and GFP generator. The promoter pCI could be repressed by BBa_C0051,the repressor CI from E.coli phage lambda.
BBa_K195614

K195614
p22
R0053

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 937 BBa_R0053 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of p22 and GFP generator. The promoter p22 could be repressed by BBa_C0053,the c2 repressor from Salmonella phage P22 .
BBa_K195615

K195615
pLuxR
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 938 BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pLuxR and GFP generator. The promoter pLuxR could be activated by LuxR in concert with HSL.
BBa_K195616

K195616
pLasR
R0079

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Reporter 1040 BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pLasR and GFP generator. The promoter pLasR could be repressed by BBa_C0079,the repressor LasR.
BBa_K195617

K195617
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1450 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pTetR with activator gene CII, and it would activate pRE to produce green fluorescent protein. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pTet and produce green fluorescent protein.
BBa_K195618

K195618
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1473 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pPenI with activator gene CII, and it would activate pRE to produce green fluorescent protein. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pPenI and produce green fluorescent protein.
BBa_K195619

K195619
pLacI
R0010

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 1596 BBa_R0010 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 This part is composed of pLacI with activator gene CII, and it would activate pRE to produce green fluorescent protein. The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The pLacI is repressed by LacI, which could be inhibited by IPTG.The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pLacI and produce green fluorescent protein.
BBa_K195620

K195620
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 896 BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 This part is composed of pRE and TetR repressor. The promoter pRE could be activated by BBa_K116602,the activator CII. TetR could repress the regulatory promoter BBa_R0040 pTet.
BBa_K195621

K195621
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 659 BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 This part is composed of pRE and PenI repressor. The promoter pRE could be activated by BBa_K116602,the activator CII. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI.
BBa_K195622

K195622
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Device 2354 BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 This part is a damped oscillator by using the regulatory promoter and the interaction between the activator, repressor and regulatory promoter. This device is produces damped oscillations between TetR and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces TetR (C0040) and GFP. The TetR then represses the pTet promoter (R0040) resulting in slower production of CII.

This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.

BBa_K195623

K195623
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
GFP
E0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Device 2140 BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 This part is a time delay device using the regulatory promoter and the interaction between the activator, repressor and regulatory promoter. This device is produces damped oscillations between PenI and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces PenI (C0074) and GFP. The PenI then represses the pPenI promoter (R0074) resulting in slower production of CII. This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.
BBa_K195624

K195624
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Device 1415 BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 This main part of the time delay device. The promoter pRE could be activated by BBa_K116602,the activator CII, which results in the production of the TetR and green fluorescent protein. TetR could repress the regulatory promoter BBa_R0040 pTet, which results in the lower production of the activator CII.
BBa_K195625

K195625
pRE
K116603

NYMU icon P.png
RBS
B0034

NYMU icon R.png
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
CII
K116602

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Device 1210 BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 This main part of the time delay device. The promoter pRE could be activated by BBa_K116602,the activator CII, which results in the production of the TetR and green fluorescent protein. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI, which results in the lower production of the activator CII.
BBa_K195626

K195626
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 665 BBa_R0040 BBa_B0034 BBa_C0074 BBa_B0015 This part is composed of pTetR, RBS,PenI and terminator. The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI. This is a subunit of toggle switch. pTet could be repressed by repressor TetR (BBa_C0040).
BBa_K195627

K195627
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 925 BBa_R0074 BBa_B0034 BBa_C0040 BBa_B0015 This part is composed of pPenI, RBS,TetR and terminator. The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet. This is a subunit of toggle switch. pPenI could be repressed by repressor PenI (BBa_C0074).
BBa_K195628

K195628
pTetR
R0040

NYMU icon P.png
RBS
B0034

NYMU icon R.png
PenI
C0074

NYMU icon C.png
Terminator
B0015

NYMU icon T.png
pPenI
R0074

NYMU icon P.png
RBS
B0034

NYMU icon R.png
TetR
C0040

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

Device 1207 BBa_R0040 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 This part is a toggle switch. The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI.The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet.
BBa_K195629

K195629
Cl lam
C0051

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

composite 912 BBa_C0051 BBa_B0015 This part is composed of cl repressor and terminator. The coding sequence cl repressor from bacteriophage lambda with LVA tail could repress BBa_R1051,the regulatory promoter pCI.
BBa_K195630

K195630
RBS
B0034

NYMU icon R.png
Cl
C0051

NYMU icon C.png
Terminator
B0015

NYMU icon T.png

generator 930 BBa_B0034 BBa_C0051 BBa_B0015 This part is composed of RBS,cl repressor and terminator. The coding sequence cl repressor from bacteriophage lambda with LVA tail could repress BBa_R1051,the regulatory promoter pCI.