Team:NYMU-Taipei/Sequences/Virus Proteins

From 2009.igem.org

Revision as of 20:43, 21 October 2009 by Eyelight (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents

Sequences and Primers

H1N1 NA

H1N1 NA orignal1419bp
ATGAACCCCAACCAGAAGATCATCACCATCGGCAGCGTGTGCATGACCATCGGCATGGCCAACCTGATCCTGCAGATCGG CAACATCATCAGCATCTGGATCAGCCACAGCATCCAGCTGGGCAACCAGAACCAGATCGAGACCTGCAACCAGAGCGTGA TCACCTACGAGAACAACACCTGGGTGAACCAGACCTACGTGAACATCAGCAACACCAACTTCGCCGCCGGCCAGAGCGTG GTGAGCGTGAAGCTGGCCGGCAACAGCAGCCTGTGCCCCGTGAGCGGCTGGGCCATCTACAGCAAGGACAACAGCGTGAG GATCGGCAGCAAGGGCGACGTGTTCGTGATCAGGGAGCCCTTCATCAGCTGCAGCCCCCTGGAGTGCAGGACCTTCTTCC TGACCCAGGGCGCCCTGCTGAACGACAAGCACAGCAACGGCACCATCAAGGACAGGAGCCCCTACAGGACCCTGATGAGC TGCCCCATCGGCGAGGTGCCCAGCCCCTACAACAGCAGGTTCGAGAGCGTGGCCTGGAGCGCCAGCGCCTGCCACGACGG CATCAACTGGCTGACCATCGGCATCAGCGGCCCCGACAACGGCGCCGTGGCCGTGCTGAAGTACAACGGCATCATCACCG ACACCATCAAGAGCTGGAGGAACAACATCCTGAGGACCCAGGAGAGCGAGTGCGCCTGCGTGAACGGCAGCTGCTTCACC GTGATGACCGACGGCCCCAGCAACGGCCAGGCCAGCTACAAGATCTTCAGGATCGAGAAGGGCAAGATCGTGAAGAGCGT GGAGATGAACGCCCCCAACTACCACTACGAGGAGTGCAGCTGCTACCCCGACAGCAGCGAGATCACCTGCGTGTGCAGGG ACAACTGGCACGGCAGCAACAGGCCCTGGGTGAGCTTCAACCAGAACCTGGAGTACCAGATCGGCTACATCTGCAGCGGC ATCTTCGGCGACAACCCCAGGCCCAACGACAAGACCGGCAGCTGCGGCCCCGTGAGCAGCAACGGCGCCAACGGCGTGAA GGGCTTCAGCTTCAAGTACGGCAACGGCGTGTGGATCGGCAGGACCAAGAGCATCAGCAGCAGGAACGGCTTCGAGATGA TCTGGGACCCCAACGGCTGGACCGGCACCGACAACAACTTCAGCATCAAGCAGGACATCGTGGGCATCAACGAGTGGAGC GGCTACAGCGGCAGCTTCGTGCAGCACCCCGAGCTGACCGGCCTGGACTGCATCAGGCCCTGCTTCTGGGTGGAGCTGAT CAGGGGCAGGCCCAAGGAGAACACCATCTGGACCAGCGGCAGCAGCATCAGCTTCTGCGGCGTGAACAGCGACACCGTGG GCTGGAGCTGGCCCGACGGCGCCGAGCTGCCCTTCACCATCGACAAGTGACTCGAGTAA

H1N1 NA mutant1419bp
ATGAACCCCAACCAGAAGATCATCACCATCGGCAGCGTGTGCATGACCATCGGCATGGCCAACCTGATCCTaCAGATCGG CAACATCATCAGCATCTGGATCAGCCACAGCATCCAGCTGGGCAACCAGAACCAGATCGAGACCTGCAACCAGAGCGTGA TCACCTACGAGAACAACACCTGGGTGAACCAGACCTACGTGAACATCAGCAACACCAACTTCGCCGCCGGCCAGAGCGTG GTGAGCGTGAAGCTGGCCGGCAACAGCAGCCTGTGCCCCGTGAGCGGCTGGGCCATCTACAGCAAGGACAACAGCGTGAG GATCGGCAGCAAGGGCGACGTGTTCGTGATCAGGGAGCCCTTCATCAGCTGtAGCCCCCTGGAGTGCAGGACCTTCTTCC TGACCCAGGGCGCCCTGCTGAACGACAAGCACAGCAACGGCACCATCAAGGACAGGAGCCCCTACAGGACCCTGATGAGC TGCCCCATCGGCGAGGTGCCCAGCCCCTACAACAGCAGGTTCGAGAGCGTGGCCTGGAGCGCCAGCGCCTGCCACGACGG CATCAACTGGCTGACCATCGGCATCAGCGGCCCCGACAACGGCGCCGTGGCCGTGCTGAAGTACAACGGCATCATCACCG ACACCATCAAGAGCTGGAGGAACAACATCCTGAGGACCCAGGAGAGCGAGTGCGCCTGCGTGAACGGCAGCTGCTTCACC GTGATGACCGACGGCCCCAGCAACGGCCAGGCCAGCTACAAGATCTTCAGGATCGAGAAGGGCAAGATCGTGAAGAGCGT GGAGATGAACGCCCCCAACTACCACTACGAGGAGTGCAGCTGCTACCCCGACAGCAGCGAGATCACCTGCGTGTGCAGGG ACAACTGGCACGGCAGCAACAGGCCCTGGGTGAGCTTCAACCAGAACCTGGAGTACCAGATCGGCTACATCTGtAGCGGC ATCTTCGGCGACAACCCCAGGCCCAACGACAAGACCGGCAGCTGCGGCCCCGTGAGCAGCAACGGCGCCAACGGCGTGAA GGGCTTCAGCTTCAAGTACGGCAACGGCGTGTGGATCGGCAGGACCAAGAGCATCAGCAGCAGGAACGGCTTCGAGATGA TCTGGGACCCCAACGGCTGGACCGGCACCGACAACAACTTCAGCATCAAGCAGGACATCGTGGGCATCAACGAGTGGAGC GGCTACAGCGGCAGCTTCGTGCAGCACCCCGAGCTGACCGGCCTGGACTGCATCAGGCCCTGCTTCTGGGTGGAGCTGAT CAGGGGCAGGCCCAAGGAGAACACCATCTGGACCAGCGGCAGCAGCATCAGCTTCTGCGGCGTGAACAGCGACACCGTGG GCTGGAGCTGGCCCGACGGCGCCGAGCTGCCCTTCACCATCGACAAGTGACTCGAGTAA

outer primer 

gaattcgcggccgcttctag ATGAACCCCAACCAGAAGA 55deg, GC:47%, 20+19=39bp
ctgcagcggccgctactagta TTACTCGAGTCACTTGTCGATG 55deg, GC:45%, 21+22=43bp

inner 1: PstI@pos70, 55deg, GC:50%, 20bp 

CTGATCCTaCAGATCGGCAA
TTGCCGATCTGtAGGATCAG

inner 2: PstI@pos369, 56deg, GC:58%, 19bp 

CTTCATCAGCTGtAGCCCC
GGGGCTaCAGCTGATGAAG

inner 3: PstI@pos951, 55deg, GC:45%, 20bp 

TACATCTGtAGCGGCATCTT
AAGATGCCGCTaCAGATGTA

H1N1 HA

H1N1 HA1701bp
ATGAAGGCCATCCTGGTGGTGCTGCTGTACACCTTCGCCACCGCCAACGCCGACACCCTGTGCATCGGCTACCACGCCA ACAACAGCACCGACACCGTGGACACCGTGCTGGAGAAGAACGTGACCGTGACCCACAGCGTGAACCTGCTGGAGGACAA GCACAACGGCAAGCTGTGCAAGCTGAGGGGCGTGGCCCCCCTGCACCTGGGCAAGTGCAACATCGCCGGCTGGATCCTG GGCAACCCCGAGTGCGAGAGCCTGAGCACCGCCAGCAGCTGGAGCTACATCGTGGAGACCCCCAGCAGCGACAACGGCA CCTGCTACCCCGGCGACTTCATCGACTACGAGGAGCTGAGGGAGCAGCTGAGCAGCGTGAGCAGCTTCGAGAGGTTCGA GATCTTCCCCAAGACCAGCAGCTGGCCCAACCACGACAGCAACAAGGGCGTGACCGCCGCCTGCCCCCACGCCGGCGCC AAGAGCTTCTACAAGAACCTGATCTGGCTGGTGAAGAAGGGCAACAGCTACCCCAAGCTGAGCAAGAGCTACATCAACG ACAAGGGCAAGGAGGTGCTGGTGCTGTGGGGCATCCACCACCCCAGCACCAGCGCCGACCAGCAGAGCCTGTACCAGAA CGCCGACACCTACGTGTTCGTGGGCAGCAGCAGGTACAGCAAGAAGTTCAAGCCCGAGATCGCCATCAGGCCCAAGGTG AGGGACCAGGAGGGCAGGATGAACTACTACTGGACCCTGGTGGAGCCCGGCGACAAGATCACCTTCGAGGCCACCGGCA ACCTGGTGGTGCCCAGGTACGCCTTCGCCATGGAGAGGAACGCCGGCAGCGGCATCATCATCAGCGACACCCCCGTGCA CGACTGCAACACCACCTGCCAGACCCCCAAGGGCGCCATCAACACCAGCCTGCCCTTCCAGAACATCCACCCCATCACC ATCGGCAAGTGCCCCAAGTACGTGAAGAGCACCAAGCTGAGGCTGGCCACCGGCCTGAGGAACATCCCCCAGATCGAGA CCAGGGGCCTGTTCGGCGCCATCGCCGGCTTCATCGAGGGCGGCTGGACCGGCATGGTGGACGGCTGGTACGGCTACCA CCACCAGAACGAGCAGGGCAGCGGCTACGCCGCCGACCTGAAGAGCACCCAGAACGCCATCGACGAGATCACCAACAAG GTGAACAGCGTGATCGAGAAGATGAACACCCAGTTCACCGCCGTGGGCAAGGAGTTCAACCACCTGGAGAAGAGGATCG AGAACCTGAACAAGAAGGTGGACGACGGCTTCCTGGACATCTGGACCTACAACGCCGAGCTGCTGGTGCTGCTGGAGAA CGAGAGGACCCTGGACTACCACGACAGCAACGTGAAGAACCTGTACGAGAAGGTGAGGAGCCAGCTGAAGAACAACGCC AAGGAGATCGGCAACGGCTGCTTCGAGTTCTACCACAAGTGCGACAACACCTGCATGGAGAGCGTGAAGAACGGCACCT ACGACTACCCCAAGTACAGCGAGGAGGCCAAGCTGAACAGGGAGGAGATCGACGGCGTGAAGCTGGAGAGCACCAGGAT CTACCAGATCCTGGCCATCTACAGCACCGTGGCCAGCAGCCTGGTGCTGGTGGTGAGCCTGGGCGCCATCAGCTTCTGG ATGTGCAGCAACGGCAGCCTGCAGTGCAGGATCTGCATCTGA
outer primer: 

gaattcgcggccgcttctag ATGAAGGCCATCCTGGTG 55deg, GC:56%, 20+18=38bp
ctgcagcggccgctactagta TCAGATGCAGATCCTGCAtTGCAGG 63deg, GC:52%, 21+25=46bp

H5N1 NA

H5N1 NA original1350bp
ATGAATCCAAATCAGAAGATAATAACCATCGGATCAATCTGTATGGTAACTGGAATAGTTAGCTTAATGTTACAAATTG GGAACATGATCTCAATATGGGTCAGTCATTCAATTCACACAGGGAATCAACACCAATCTGAACCAATCAGCAATACTAA TTTTCTTACTGAGAAAGCTGTGGCTTCAGTAAAATTAGCGGGCAATTCATCTCTTTGCCCCATTAACGGATGGGCTGTA TACAGTAAGGACAACAGTATAAGGATCGGTTCCAAGGGGGATGTGTTTGTTATAAGAGAGCCGTTCATCTCATGCTCCC ACTTGGAATGCAGAACTTTCTTTTTGACTCAGGGAGCCTTGCTGAATGACAAGCACTCCAATGGGACTGTCAAAGACAG AAGCCCTCACAGAACATTAATGAGTTGTCCTGTGGGTGAGGCTCCCTCCCCATATAACTCAAGGTTTGAGTCTGTTGCT TGGTCAGCAAGTGCTTGCCATGATGGCACCAGTTGGTTGACGATTGGAATTTCTGGCCCAGACAATGGGGCTGTGGCTG TATTGAAATACAATGGCATAATAACAGACACTATCAAGAGTTGGAGGAACAACATACTGAGAACTCAAGAGTCTGAATG TGCATGTGTAAATGGCTCTTGCTTTACTGTAATGACTGACGGACCAAGTAATGGTCAGGCATCACATAAGATCTTCAAA ATGGAAAAAGGGAAAGTGGTTAAATCAGTCGAATTGGATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTA ATGCCGGAGAAATCACATGTGTGTGCAGGGATAATTGGCATGGCTCAAATCGGCCATGGGTATCTTTCAATCAAAATTT GGAGTATCAAATAGGATATATATGCAGTGGAGTTTTCGGAGACAATCCACGCCCCAATGATGGAACAGGTAGTTGTGGT CCGGTGTCCTCTAACGGGGCATATGGGGTAAAAGGGTTTTCATTTAAATACGGCAATGGTGTCTGGATCGGGAGAACCA AAAGCACTAATTCCAGGAGCGGCTTTGAAATGATTTGGGATCCAAATGGGTGGACTGAAACGGACAGTAGCTTTTCAGT GAAACAAGATATCGTAGCAATAACTGATTGGTCAGGATATAGCGGGAGTTTTGTCCAGCATCCAGAACTGACAGGACTA GATTGCATAAGACCTTGTTTCTGGGTTGAGTTGATCAGAGGGCGGCCCAAAGAGAGCACAATTTGGACTAGTGGGAGCA GCATATCTTTTTGTGGTGTAAATAGTGACACTGTGGGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTCACCATTGA CAAGTAG

H5N1 NA mutant1350bp
ATGAATCCAAATCAGAAGATAATAACCATCGGATCAATCTGTATGGTAACTGGAATAGTTAGCTTAATGTTACAAATTG GGAACATGATCTCAATATGGGTCAGTCATTCAATTCACACAGGGAATCAACACCAATCTGAACCAATCAGCAATACTAA TTTTCTTACTGAGAAAGCTGTGGCTTCAGTAAAATTAGCGGGCAATTCATCTCTTTGCCCCATTAACGGATGGGCTGTA TACAGTAAGGACAACAGTATAAGGATCGGTTCCAAGGGGGATGTGTTTGTTATAAGAGAGCCGTTCATCTCATGCTCCC ACTTGGAATGCAGAACTTTCTTTTTGACTCAGGGAGCCTTGCTGAATGACAAGCACTCCAATGGGACTGTCAAAGACAG AAGCCCTCACAGAACATTAATGAGTTGTCCTGTGGGTGAGGCTCCCTCCCCATATAACTCAAGGTTTGAGTCTGTTGCT TGGTCAGCAAGTGCTTGCCATGATGGCACCAGTTGGTTGACGATTGGAATTTCTGGCCCAGACAATGGGGCTGTGGCTG TATTGAAATACAATGGCATAATAACAGACACTATCAAGAGTTGGAGGAACAACATACTGAGAACTCAAGAGTCTGAATG TGCATGTGTAAATGGCTCTTGCTTTACTGTAATGACTGACGGACCAAGTAATGGTCAGGCATCACATAAGATCTTCAAA ATGGAAAAAGGGAAAGTGGTTAAATCAGTCGAATTGGATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTA ATGCCGGAGAAATCACATGTGTGTGCAGGGATAATTGGCATGGCTCAAATCGGCCATGGGTATCTTTCAATCAAAATTT GGAGTATCAAATAGGATATATATGCAGTGGAGTTTTCGGAGACAATCCACGCCCCAATGATGGAACAGGTAGTTGTGGT CCGGTGTCCTCTAACGGGGCATATGGGGTAAAAGGGTTTTCATTTAAATACGGCAATGGTGTCTGGATCGGGAGAACCA AAAGCACTAATTCCAGGAGCGGCTTTGAAATGATTTGGGATCCAAATGGGTGGACTGAAACGGACAGTAGCTTTTCAGT GAAACAAGATATCGTAGCAATAACTGATTGGTCAGGATATAGCGGGAGTTTTGTCCAGCATCCAGAACTGACAGGACTA GATTGCATAAGACCTTGTTTCTGGGTTGAGTTGATCAGAGGGCGGCCCAAAGAGAGCACAATTTGGACcAGTGGGAGCA GCATATCTTTTTGTGGTGTAAATAGTGACACTGTGGGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTCACCATTGA CAAGTAG

outer primer: 

gaattcgcggccgcttctag ATGAATCCAAATCAGAAGATAATAACCA 55deg, GC:29%, 20+28=48bp
ctgcagcggccgctactagta CTACTTGTCAATGGTGAATGGC 55deg, GC:45%, 21+22=43bp

inner primer: SpeI@pos1252, 56deg, GC:56%, 18bp 

ATTTGGACcAGTGGGAGC
GCTCCCACTgGTCCAAAT

H5N1 HA

H5N1 HA original1701bp
ATGGAGAAAATAGTGCTTCTTTTTGCAATAGTCAGTCTTGTTAAAAGTGATCAGATTTGCATTGGTTACCATGCAAACA ACTCGACAGAGCAGGTTGACACAATAATGGAAAAGAACGTTACTGTTACACATGCCCAAGACATACTGGAAAAGAAACA CAACGGGAAGCTCTGCGATCTAGATGGAGTGAAGCCTCTAATTTTGAGAGATTGTAGCGTAGCTGGATGGCTCCTCGGA AACCCAATGTGTGACGAATTCATCAATGTGCCGGAATGGTCTTACATAGTGGAGAAGGCCAATCCAGTCAATGACCTCT GTTACCCAGGGGATTTCAATGACTATGAAGAATTGAAACACCTATTGAGCAGAATAAACCATTTTGAGAAAATTCAGAT CATCCCCAAAAGTTCTTGGTCCAGTCATGAAGCCTCATTAGGGGTGAGCTCAGCATGTCCATACCAGGGAAAGTCCTCC TTTTTCAGAAATGTGGTATGGCTTATCAAAAAGAACAGTACATACCCAACAATAAAGAGGAGCTACAATAATACCAACC AAGAAGATCTTTTGGTACTGTGGGGGATTCACCATCCTAATGATGCGGCAGAGCAGACAAAGCTCTATCAAAACCCAAC CACCTATATTTCCGTTGGGACATCAACACTAAACCAGAGATTGGTACCAAGAATAGCTACTAGATCCAAAGTAAACGGG CAAAGTGGAAGGATGGAGTTCTTCTGGACAATTTTAAAGCCGAATGATGCAATCAACTTCGAGAGTAATGGAAATTTCA TTGCTCCAGAATATGCATACAAAATTGTCAAGAAAGGGGACTCAACAATTATGAAAAGTGAATTGGAATATGGTAACTG CAACACCAAGTGTCAAACTCCAATGGGGGCGATAAACTCTAGCATGCCATTCCACAATATACACCCTCTCACCATTGGG GAATGCCCCAAATATGTGAAATCAAACAGATTAGTCCTTGCGACTGGGCTCAGAAATAGCCCTCAAAGAGAGAGAAGAA GAAAAAAGAGAGGATTATTTGGAGCTATAGCAGGTTTTATAGAGGGAGGATGGCAGGGAATGGTAGATGGTTGGTATGG GTACCACCATAGCAATGAGCAGGGGAGTGGGTACGCTGCAGACAAAGAATCCACTCAAAAGGCAATAGATGGAGTCACC AATAAGGTCAACTCGATCATTGACAAAATGAACACTCAGTTTGAGGCCGTTGGAAGGGAATTTAACAACTTAGAAAGGA GAATAGAGAATTTAAACAAGAAGATGGAAGACGGGTTCCTAGATGTCTGGACTTATAATGCTGAACTTCTGGTTCTCAT GGAAAATGAGAGAACTCTAGACTTTCATGACTCAAATGTCAAGAACCTTTACGACAAGGTCCGACTACAGCTTAGGGAT AATGCAAAGGAGCTGGGTAACGGTTGTTTCGAGTTCTATCATAAATGTGATAATGAATGTATGGAAAGTGTAAGAAATG GAACGTATGACTACCCGCAGTATTCAGAAGAAGCGAGACTAAAAAGAGAGGAAATAAGTGGAGTAAAATTGGAATCAAT AGGAATTTACCAAATACTGTCAATTTATTCTACAGTGGCGAGTTCCCTAGCACTGGCAATCATGGTAGCTGGTCTATCC TTATGGATGTGCTCCAATGGGTCGTTACAATGCAGAATTTGCATTTAA

H5N1 HA mutant1701bp
ATGGAGAAAATAGTGCTTCTTTTTGCAATAGTCAGTCTTGTTAAAAGTGATCAGATTTGCATTGGTTACCATGCAAACA ACTCGACAGAGCAGGTTGACACAATAATGGAAAAGAACGTTACTGTTACACATGCCCAAGACATACTGGAAAAGAAACA CAACGGGAAGCTCTGCGATCTAGATGGAGTGAAGCCTCTAATTTTGAGAGATTGTAGCGTAGCTGGATGGCTCCTCGGA AACCCAATGTGTGACGAgTTCATCAATGTGCCGGAATGGTCTTACATAGTGGAGAAGGCCAATCCAGTCAATGACCTCT GTTACCCAGGGGATTTCAATGACTATGAAGAATTGAAACACCTATTGAGCAGAATAAACCATTTTGAGAAAATTCAGAT CATCCCCAAAAGTTCTTGGTCCAGTCATGAAGCCTCATTAGGGGTGAGCTCAGCATGTCCATACCAGGGAAAGTCCTCC TTTTTCAGAAATGTGGTATGGCTTATCAAAAAGAACAGTACATACCCAACAATAAAGAGGAGCTACAATAATACCAACC AAGAAGATCTTTTGGTACTGTGGGGGATTCACCATCCTAATGATGCGGCAGAGCAGACAAAGCTCTATCAAAACCCAAC CACCTATATTTCCGTTGGGACATCAACACTAAACCAGAGATTGGTACCAAGAATAGCTACTAGATCCAAAGTAAACGGG CAAAGTGGAAGGATGGAGTTCTTCTGGACAATTTTAAAGCCGAATGATGCAATCAACTTCGAGAGTAATGGAAATTTCA TTGCTCCAGAATATGCATACAAAATTGTCAAGAAAGGGGACTCAACAATTATGAAAAGTGAATTGGAATATGGTAACTG CAACACCAAGTGTCAAACTCCAATGGGGGCGATAAACTCTAGCATGCCATTCCACAATATACACCCTCTCACCATTGGG GAATGCCCCAAATATGTGAAATCAAACAGATTAGTCCTTGCGACTGGGCTCAGAAATAGCCCTCAAAGAGAGAGAAGAA GAAAAAAGAGAGGATTATTTGGAGCTATAGCAGGTTTTATAGAGGGAGGATGGCAGGGAATGGTAGATGGTTGGTATGG GTACCACCATAGCAATGAGCAGGGGAGTGGGTACGCTGCcGACAAAGAATCCACTCAAAAGGCAATAGATGGAGTCACC AATAAGGTCAACTCGATCATTGACAAAATGAACACTCAGTTTGAGGCCGTTGGAAGGGAATTTAACAACTTAGAAAGGA GAATAGAGAATTTAAACAAGAAGATGGAAGACGGGTTCCTAGATGTCTGGACTTATAATGCTGAACTTCTGGTTCTCAT GGAAAATGAGAGAACTCTgGACTTTCATGACTCAAATGTCAAGAACCTTTACGACAAGGTCCGACTACAGCTTAGGGAT AATGCAAAGGAGCTGGGTAACGGTTGTTTCGAGTTCTATCATAAATGTGATAATGAATGTATGGAAAGTGTAAGAAATG GAACGTATGACTACCCGCAGTATTCAGAAGAAGCGAGACTAAAAAGAGAGGAAATAAGTGGAGTAAAATTGGAATCAAT AGGAATTTACCAAATACTGTCAATTTATTCTACAGTGGCGAGTTCCCTAGCACTGGCAATCATGGTAGCTGGTCTATCC TTATGGATGTGCTCCAATGGGTCGTTACAATGCAGAATTTGCATTTAA

outer primer: 

gaattcgcggccgcttctag ATGGAGAAAATAGTGCTTCTTTTTG 54deg, GC:32%, 20+25=45bp
ctgcagcggccgctactagta TTAAATGCAAATTCTGCATTGTAACG 55deg, GC:31%, 21+26=47bp

inner primer1: EcoRI@pos253, 54deg, GC:43%, 21bp 

TGTGTGACGAgTTCATCAATG
CATTGATGAAcTCGTCACACA

inner primer2: PstI@pos1142, 55deg, GC:50%, 18bp 

TACGCTGCcGACAAAGAA
TTCTTTGTCgGCAGCGTA

inner primer3: XbaI@pos1359, 55deg, GC:43%, 23bp 

GAGAGAACTCTgGACTTTCATGA
TCATGAAAGTCcAGAGTTCTCTC

HIV-1 gp120

HIV-1 gp120 original2580bp
ATGAGAGTGACGGGGATCAGGAAGAATTATCGGCACTTATGGAAATGGGGCACCATGCTCCTTGGAATGTTGATGATCT GTAGTGCTGTAGAAAACTTGTGGGTCACAGTCTATTATGGGGTACCTATATGGAAAGATGCAACCACCACTTTATTTTG TGCATCAGATGCTAGAGCATATGATACAGAGGTACATAATGTTTGGGCTACACATGCCTGTGTACCCACAGACCCCAAC CCACAAGAACTGGTTTTGGTAAATGTAACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGATCAGATGCATGAAG ATGTAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCACTTTAAACTGTACAAA TGCTACCCGTAGCAGTGATGATACCAGAAATAGCACTGAGAACATAGAGGGAATGAAAAATTGCTCTTTCAATGCAACC ACAGTAGTAAGAGATAAGCAGCAGAAAGTGTCTGCACTTTTTTATAGACTTGATATAGTACCACTTACTGAGAAGGAAT CTAGTGAGAACTCTAGTGGGTATTATAGATTAATAAATTGTAATACCTCAGCCATAACACAAGCCTGTCCAAAGGTTAC TTTTGATCCAATTCCTATACACTATTGTACTCCAGCTGGTTATGCAATTCTAAAGTGTAATAATAAGACATTCAATGGG ACAGGGCCATGCTCTAATGTTAGCACAGTACAATGTACACATGGGATTAAGCCAGTGGTATCAACTCAACTACTGTTAA ATGGTAGCCTATCAGAAGGAGGAATAATAATTAGATCTGAAAATCTGACAGACAATGTCAAAACAATAATAGTACATCT TAATGAATCTGTAGGAATTGTATGTACAAGACCCAACAATAATACAAGAAAAAGTATAAGGATAGGACCAGGACAAACA TTCTATGCAACAGGAGAAATAATAGGAGACATAAGACAAGCACATTGTAACATTAGTGAATATAAATGGAATAAAACTT TGCAAAGGGTAAGTGAAAAATTAGCAGAATACTTCCCGAATGACACAATAAAATTTGCACCATCCTCAGGAGGGGACCT AGAAATTACAACACATAGCTTTAATTGTAGAGGAGAATTTTTCTATTGTAATACATCAGGCCTGTTTAATGGTACATAT AATGGTACATACAATGCTAATGGTACAGAAAACGCAACCATCACAATCCCATGCAGAATAAAGCAAATTATAAATATGT GGCAGGAGGTAGGAAGAGCAATGTATGCCCCTCCCATTGCAGGAAACATAACATGTAACTCAAGTATCACAGGACTACT ATTGGTACGTGATGGAGGAATAAATAATACAAATAATACAGAGACATTCAGACCTGGAGGAGGAGATATGAAGGACAAT TGGAGAAGTGAATTATACAAATATAAAGTAGTAGAAGTTAAACCATTGGGAGTAGCACCCACTACAGCAAAAAGGAGAG TGGTGGAGAGAGAAAAAAGGGGAATAGGAATAGGAGCTGTGTTCCTTGGGTTCTTGGGAGTAGCAGGAAGCACTATGGG CGCGGCGTCTGTAACGCTGACGGTACAGGCCAGACAATTGCTGTCTGGTATAGTGCAACAGCAAAGCAATTTGCTGAAG GCTATAGAGGCGCAACAGCATCTGTTGCAACTCACGGTCTGGGGCATTAAGCAGCTCCAGACAAGAGTCCTGGCTATAG AAAGATACCTAAAGGATCAACAGCTCCTAGGGATTTGGGGTTGCTCTGGAAAAATCATCTGCACCACTGCTGTACCTTG GAACTCCAGTTGGAGTAACAAGTCTCAACAAGATATTTGGGATAACATGACCTGGATGCAATGGGATAAAGAAATTAGT AATTACACAAACACAATATACAGTTTGCTTGAAGAATCGCAAATCCAGCAGGAGAAGAATGAAAAAGATCTATTAGCAT TGGACAGCTGGAAAAATCTATGGAATTGGTTTAACATATCAAATTGGCTGTGGTATATAAAAATATTCATAATAATAGT AGGAAGCTTGATAGGTTTAAGAATAATTTTTGCTGTGCTCTCTATAGTGAATAGAGTTAGGCAGGGATACTCACCTTTG TCGTTTCAGATCCTTACCCCGAACCCAGGGGGACCCGACAGGCTCGGAAGAATCGAAGAAGAAGGTGGAGAGCAAGACA AAGGAAGATCCATTCGATTAGTGAACGGATTCTTTGCGCTTGCCTGGGACGACCTGCGGAACCTGTGCCTCTTCAGCTA CCACCGCTTGAGAGACTTCATATTAGTGGCAGCGAGAGTGGTGGAACTTCTGGGACGCAGCAGTCTCAGGGGACTACAG AGGGGGTGGGAAGCCCTTAAATATCTGGGAAGTCTTGTGCAGTACTGGGGTCAGGAGCTAAAAAAGAGTGCTATTAGTC TGTTTGATGCCATAGCAATAGCAGTAGCTGAAGGAACAGATAGGATTATAGAATTCATACAAAGATTTTGTAGAGCTAT CTACAACATACCTAGAAGAATAAGACAGGGATTTGAAGCAGCTTTGCAATAA

HIV-1 gp120 original2580bp
ATGAGAGTGACGGGGATCAGGAAGAATTATCGGCACTTATGGAAATGGGGCACCATGCTCCTTGGAATGTTGATGATCT GTAGTGCTGTAGAAAACTTGTGGGTCACAGTCTATTATGGGGTACCTATATGGAAAGATGCAACCACCACTTTATTTTG TGCATCAGATGCTAGAGCATATGATACAGAGGTACATAATGTTTGGGCTACACATGCCTGTGTACCCACAGACCCCAAC CCACAAGAACTGGTTTTGGTAAATGTAACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGATCAGATGCATGAAG ATGTAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCACTTTAAACTGTACAAA TGCTACCCGTAGCAGTGATGATACCAGAAATAGCACTGAGAACATAGAGGGAATGAAAAATTGCTCTTTCAATGCAACC ACAGTAGTAAGAGATAAGCAGCAGAAAGTGTCTGCACTTTTTTATAGACTTGATATAGTACCACTTACTGAGAAGGAAT CTAGTGAGAACTCTAGTGGGTATTATAGATTAATAAATTGTAATACCTCAGCCATAACACAAGCCTGTCCAAAGGTTAC TTTTGATCCAATTCCTATACACTATTGTACTCCAGCTGGTTATGCAATTCTAAAGTGTAATAATAAGACATTCAATGGG ACAGGGCCATGCTCTAATGTTAGCACAGTACAATGTACACATGGGATTAAGCCAGTGGTATCAACTCAACTACTGTTAA ATGGTAGCCTATCAGAAGGAGGAATAATAATTAGATCTGAAAATCTGACAGACAATGTCAAAACAATAATAGTACATCT TAATGAATCTGTAGGAATTGTATGTACAAGACCCAACAATAATACAAGAAAAAGTATAAGGATAGGACCAGGACAAACA TTCTATGCAACAGGAGAAATAATAGGAGACATAAGACAAGCACATTGTAACATTAGTGAATATAAATGGAATAAAACTT TGCAAAGGGTAAGTGAAAAATTAGCAGAATACTTCCCGAATGACACAATAAAATTTGCACCATCCTCAGGAGGGGACCT AGAAATTACAACACATAGCTTTAATTGTAGAGGAGAATTTTTCTATTGTAATACATCAGGCCTGTTTAATGGTACATAT AATGGTACATACAATGCTAATGGTACAGAAAACGCAACCATCACAATCCCATGCAGAATAAAGCAAATTATAAATATGT GGCAGGAGGTAGGAAGAGCAATGTATGCCCCTCCCATTGCAGGAAACATAACATGTAACTCAAGTATCACAGGACTACT ATTGGTACGTGATGGAGGAATAAATAATACAAATAATACAGAGACATTCAGACCTGGAGGAGGAGATATGAAGGACAAT TGGAGAAGTGAATTATACAAATATAAAGTAGTAGAAGTTAAACCATTGGGAGTAGCACCCACTACAGCAAAAAGGAGAG TGGTGGAGAGAGAAAAAAGGGGAATAGGAATAGGAGCTGTGTTCCTTGGGTTCTTGGGAGTAGCAGGAAGCACTATGGG CGCGGCGTCTGTAACGCTGACGGTACAGGCCAGACAATTGCTGTCTGGTATAGTGCAACAGCAAAGCAATTTGCTGAAG GCTATAGAGGCGCAACAGCATCTGTTGCAACTCACGGTCTGGGGCATTAAGCAGCTCCAGACAAGAGTCCTGGCTATAG AAAGATACCTAAAGGATCAACAGCTCCTAGGGATTTGGGGTTGCTCTGGAAAAATCATCTGCACCACTGCTGTACCTTG GAACTCCAGTTGGAGTAACAAGTCTCAACAAGATATTTGGGATAACATGACCTGGATGCAATGGGATAAAGAAATTAGT AATTACACAAACACAATATACAGTTTGCTTGAAGAATCGCAAATCCAGCAGGAGAAGAATGAAAAAGATCTATTAGCAT TGGACAGCTGGAAAAATCTATGGAATTGGTTTAACATATCAAATTGGCTGTGGTATATAAAAATATTCATAATAATAGT AGGAAGCTTGATAGGTTTAAGAATAATTTTTGCTGTGCTCTCTATAGTGAATAGAGTTAGGCAGGGATACTCACCTTTG TCGTTTCAGATCCTTACCCCGAACCCAGGGGGACCCGACAGGCTCGGAAGAATCGAAGAAGAAGGTGGAGAGCAAGACA AAGGAAGATCCATTCGATTAGTGAACGGATTCTTTGCGCTTGCCTGGGACGACCTGCGGAACCTGTGCCTCTTCAGCTA CCACCGCTTGAGAGACTTCATATTAGTGGCAGCGAGAGTGGTGGAACTTCTGGGACGCAGCAGTCTCAGGGGACTACAG AGGGGGTGGGAAGCCCTTAAATATCTGGGAAGTCTTGTGCAGTACTGGGGTCAGGAGCTAAAAAAGAGTGCTATTAGTC TGTTTGATGCCATAGCAATAGCAGTAGCTGAAGGAACAGATAGGATTATAGAgTTCATACAAAGATTTTGTAGAGCTAT CTACAACATACCTAGAAGAATAAGACAGGGATTTGAAGCAGCTTTGCAATAA

outer primer: 

gaattcgcggccgcttctag ATGAGAGTGACGGGGATC 54deg, GC:56%, 20+18=38bp
ctgcagcggccgctactagta TTATTGCAAAGCTGCTTCAAATC 54deg, GC:35%, 21+23=44bp

inner primer: EcoRI@pos2500, 54deg, GC:28%, 32bp 

GATAGGATTATAGAgTTCATACAAAGATTTTG 
CAAAATCTTTGTATGAAcTCTATAATCCTATC

adenovirus type 13 penton base

  • the same as type 3 
       1 atgaggcgcg cggtggtgtc ttcctctcct cctccctcgt acgagagcgt gatggcgcag
      61 gcgaccctgg aggttccgtt tgtgcctccg cggtatatgg ctcctacgga gggcagaaac
     121 agcattcgtt actcggagct ggctccgcag tacgacacca ctcgcgtgta cttggtggac
     181 aacaagtcgg cggacatcgc ttccctgaac taccaaaacg accacagcaa cttcctgacc
     241 acggtggtgc agaacaacga tttcaccccc gccgaggcca gcacgcagac gataaatttt
     301 gacgagcggt cgcggtgggg cggtgatctg aagaccattc tgcacaccaa catgcccaat
     361 gtgaacgagt acatgttcac cagcaagttt aaggcgcggg tgatggtggc taggaagcac
     421 cccaaagatg tgcaggatcc taatgataga agcaaggata tcttagagta taagtggttt
     481 gagtttaccc tgcccgaggg caacttttcc gagaccatga ccatagacct gatgaacaac
     541 gccatcttgg aaaactactt gcaagtgggg cggcaaaatg gcgtgctgga gagtgatatc
     601 ggagtcaagt ttgacagcag gaatttcaga ctgggctggg acccggagac caagctggta
     661 atgccggggg tctacaccta cgaggccttc cacccggacg tggtgctact gccgggctgc
     721 ggggtggact tcaccgagag ccgcctgagc aacctcctgg gcattcgcaa gaagcaacct
     781 tttcaagagg gcttcagaat catgtatgag gatctagaag ggggcaacat ccctgccctg
     841 ctggatgtgg atgcatacct caaaagcaag aatgatctgg aagaggctac caaaaatgca
     901 aacagagctg ctgctaatgg aggtggtgaa actaggggag atacttttct caccaccgaa
     961 cagctgagag ccgctggcaa ggagctggtt attaagccaa tcaaggaaga tgctagcaaa
    1021 aggagctata atgtcataga tgacacccat gacacgctgt accgaagctg gtacctgtcc
    1081 tacacatacg gggaccccga gaagggagtg cagtcgtgga cgctgctcac caccccggac
    1141 gtcacctgcg gcgcggagca agtctactgg tcgctgccgg acctcatgca agaccccgtc
    1201 accttccgct ctacccagca agtcagcaac taccccgtgg tcggcgccga gctcatgcct
    1261 ttccgcgcca agagctttta caacgacctc gccgtctact cccagctcat ccgcagctac
    1321 acctccctca cccacgtctt caaccgcttc cccgacaacc agatcctctg ccgcccgccc
    1381 gcgcccacca tcaccaccgt cagtgaaaac gtgcctgctc tcacagatca cgggacgcta
    1441 ccgctgcgca gcagtatccg cggagtccag cgagtgaccg tcactgacgc ccgtcgccgc
    1501 acctgtccct acgtctacaa ggccctgggc atagtcgcgc cgcgcgtgct ctctagtcgc
    1561 accttt

adenovirus type 13 fiber protein

       1 ctttggacaa cacctgatcc atcgcccaat tgtaaagccg aaacagaaaa ggactcaaag
      61 cttactttgg ttctaacaaa atgcggaagt caaatcttag ctactgtgtc tattattgtt
     121 cttaagggta aatatgaatt tgttaaaaaa gaaacagagc ctaagtcctt tgatgttaaa
     181 ttgctgtttg acagcaaagg agtgcttcta ccaacttcaa atctttccaa agaatattgg
     241 aactatagaa gttatgataa caacatagga actccttatg aaaatgcagt tccatttatg
     301 ccaaatctaa aagcttatcc aaaaccaact aaaactgctt cagataaagc agaaaataaa
     361 ataagcagtg ctaaaaacaa aattgtaagc aacttctact ttggagggca agcatatcaa
     421 cctgggacaa taataattaa gtttaatgag gaaatagatg agacatgtgc atactctatc
     481 acctttaact ttggttgggg caaggtatat gacaatccat ttccatttga taccacctct
     541 tttaccttct cttatattgc ccaagaaaat gaagacaaag actaa
  • primer for adenovirus penton base and fiber protein link

F:gcttctag atgaggcgcgcgg 55deg,77%GC, 8+13bp
R:ctgcagcggccgctactagta ttagtctttgtcttcattttcttggg 55deg, 35%GC 21+26bp

Retrieved from "http://2009.igem.org/Team:NYMU-Taipei/Sequences/Virus_Proteins"