Team:Newcastle/Labwork/13 August 2009

From 2009.igem.org

Revision as of 14:19, 20 October 2009 by MJR09 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG

Formal Lab Session - 13th August 2009

Overview


Sporulation Tuning/Chassis Team

Summary

The plates for our test transformation.

Yesterday's germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.

Therefore, on Monday, we intend to redo the experiment. See Monday, 17th August for more details.

Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector using the Transformation Protocol as well as following the changes which the Metal Sensor Team implemented. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.

Results

Following an illustration which clearly explains what to plate out from the Bacillus Transformation Protocol, we plated 4 plates, 3 of which contained the antibiotic chloramphenicol, and LB, and 1 containing just LB. The plate which contained just LB was the control plate.

The following pictures show the results of our team's transformations, which were a success.

The transformation control plate.

No colonies were expected to grow on this plate as no DNA was added to the cells for transformation

The transformation control (water) plate.

Instead of adding DNA, water was added instead. Therefore, no colonies should be growing on this plate either.


The transformation 50ul plate. The transformation 200ul plate. Looking at the two pictures above, it shows the transformation has been a success as colonies were growing on the plates where DNA was added. The plate where 200ul of the transformed bacteria were added, had more colonies growing as expected.

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31



News

Events

Social Net

  • Newcastle iGEM Twitter
  • Newcastle on Facebook
  • Newcastle Youtube Channel