Team:Newcastle/Meetings/3 September 2009

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Contents 1 03 September 2009 - iGEM Meeting 1.1 Agenda 1.2 Action Points during last meeting 1.3 Minutes 1.3.1 Reviewing last meeting's Action Points

03 September 2009 - iGEM Meeting

• Chair: Mat
• Students: Mat, Jess Goksel, James, Hanny, Arun
• Minutes: James (in Jane's absence)
• Instructors: Neil, Jen
• Advisors: Morgan

Agenda

• Review last week's action points
• Lab summary
• Questions
• Next week's lab plan

Action Points during last meeting

• Need some sequencing primers for each BioBrick
• Tidy up some of the cloning strategies
• Either put the virtual constructs on the hard drive of the computer in Phil's office OR place them onto a shared network
• Need to review timeline
• Each team needs to come up with a five minute summary of their sub-project
• Ask Nigel Robinson's advice on how to help Cornell
• E-mail UQ University and enquire about helping them with modelling
• Negotiate swap with Cornell and enquire about modelling
• Follow up Groningen's e-mail
• T-shirts designs
• Integration Vector?
• Promoter library?

Minutes

Reviewing last meeting's Action Points

• Need some sequencing primers for each BioBrick
• Tidy up some of the cloning strategies
  • Put current versions on the wiki, even if they are not finished; just to have them in one place.
• Either put the virtual constructs on the hard drive of the computer in Phil's office OR place them onto a shared network
  • use Dropbox (getdropbox.com)
• Need to review timeline
  • Students to ammend timeline.
• Team members
  • Hanny has returned, after handing in her work.
  • Jane has left for a period of one month.
  • Jen has returned.
• Each team came up with a five minute summary of their sub-project, James, Mat and Jess
  • 5 Minute kit has arrived.
  • Some of the PCRs are not working.
    • Use a different Taq.
    • Redesign primers, maybe bigger and without cut sites (2 days).
  • Use less genomic DNA.
  • More ethanol is needed (Chris is to be reminded next wednesday).
  • Only small gloves are currently available in the lab - we are to order some.
  • Ask Nigel Robinson about working with Cadmium, and an electromicroscope.
• Ask Nigel Robinson's advice on how to help Cornell
  • Mat emailing him; he has indirectly assisted Groningen.
• E-mail UQ University and enquire about helping them with modelling
  • Mat to email.
  • Modellers are to meet to discuss and work.
• Negotiate swap with Cornell and enquire about modelling
  • Mat to email?
  • Modellers are to meet to discuss and work.
• Follow up Groningen's e-mail
  • Mat to email?
• T-shirts designs
  • Numbers of shits - 11 are going to Jamboree, plus other members, backups, etc.
  • Personalise some of the shirts - make name or initials look like plasmid ID.
  • Purple is a possible colour.
  • Design needed soon.
• Poster
  • Can be no larger than A0 (1.2m²)
  • Look at possible design layouts.
  • Must be sent for printing 2 weeks before Jamboree.
• Presentation
  • Mock presentations are to be given to:
    • The Bioinformatics research group on a Friday morning in late October.
    • Noel's student group in Sunderland.
  • Prestenation should be around 20 mins; in a 30 min time slot.
  • 10-20 slides.
  • Come up with our content, before designing slides
  • Use Camtasia to record models, etc.
  • Some parts are to be simplified (eg. gene names), more tech parts to be kept (eg. equations).
  • Try to focus on the Biology, not the 'experience'.
• Integration Vector?
• Promoter library?
  • Mat is working under Neil and Jen's supervision on this in the lab; cloning into pgfp, etc.
• Sequence the AND gate?
  • Needed for Tuesday.
• Boston
  • Research ESTA for USA trip.
  • Neil to send out full details of flights and hotels.
• Noel's microscope in Sunderland.
  • Goksel to be trained.
• Lab rota
  • New rota to be devised.
  • Current 'free-for-all' cannot continue.
  • Teams are to take time out of lab to work on t-shirts, posters, modelling, etc.
• Labwork
  • Lab work is to continue at fast pace.
  • Shared labbooks are to remain in the lab.
  • Gel UV images are to be placed in Dropbox.
  • Print out photos of gels, etc, and stick into lab books.
  • Use the lab scanner for copying lab book content for wiki.
  • Neil to send off Plasmids.
  • Various PCR primers and enzymes have arrived.