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8 October 2009 - iGEM Meeting

• Chair: Mat
• Students: Jess, Jane, James, Hanny, Goksel, Mat, Arun
• Minutes: James
• Instructors: Jen, Neil
• Advisors: Dan, Morgan
• Guests: Zoltan

Agenda

• General News
• Review last week's general action points
• Review last week's specialist action points
• Give summaries in the following areas:
  • Lab Work (Goksel and Hanny)
  • Wiki (Mat)
  • Presentation (Arun)
  • Poster (Jess)
  • Modelling (James)
  • T-shirt/Marketing (Jess)
• Questions

Last Week's General Action Points

• Design sequencing primers for pMutin4 and GFP-rrnB (Arun)
• Matt Pocock needs to fill in ESTA form
• Create a page on the wiki about ethics (Mathew)
• Fill Valencia's questionnaire (everyone)
• Get Hanny guest student card
• Search for other websites for those green "bugs" (Jess)
• Contact Nigel Robinson to use his lab and the electronmicroscope
• Citate the papers in the wiki for metal sensing. Quote short sentences where necessary.
• Goksel to send DNA off to Parts Registry.

Last Week's Specialist Action Points

Lab

• Hanny to sequence BioBricks

Wiki

• Need to update lab work section
• Need to study Imperial 2008 iGEM wiki's video for ideas.
• Redraw and add colour to diagram on front page currently (the process chart) - possibly using Omnigraffle for Mac
• The "Eco Warrior" story, which is currently on the front page of the wiki, needs to go in the 'Fun and Games' section.
• Put a diagram of a Bacillus subtilis' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
• Make a date for Sunday 11th October for students meetup to take photos around the university/city.

Presentation

• Sub-project teams need to send at least two slides with some information on them.
• Presentation practice to be held on 23rd October - times to be decided

Poster

• Jess needs to update team by next week
• Each team to create document for the poster creation.

Modelling

• Make a date for a Modelling meeting to work out parameter values, and how to integrate into population model.

T-shirt/Marketing

• Get t-shirt designs to printing company

Minutes

• Introduced the team to Zoltan

Labwork

• Hanny - kinA worked, now in E.coli, transforming into B.subtilis - need to make sure it has gone into amyE - plating out onto starch and IPGT plates looking for spores and halos - if done, characterised!
• Goksel - cotC, re-PCRed, re-cut into pMutin4 - should work now.
• Stocastic Switch still stuck at 85% complete.
• Metal Sensor also stuck at 85% complete.
• Safety Officer visted the lab - Arun, Mat and Hanny were not wearing lab coats - Mat and Arun should not have been there.
• Goksel is to go on some safety courses, if he is to be in the lab in the future.
• Sequence Biobricks - not done yet - tell Phil that we will pay.
• Request for variance still pending.
• May need to clone kinA into Biobrick compatible vector.

On last weeks APs

• For pMutin4 sequencing primers, just use pMutin2 sequence.
• Dan emailed Matt Pocock to check if he has done the ESTA.
• Mat still needs to do the ethics page.
• Goksel and Jess still need to do Valencia's questionairre.
• Hanny still needs a guest card.
• Could make our own green bugs
• Nigel Robinson replied - need to ask him about other metals.
• Need to cite papers.
• Prepare DNA for submission - watch for postal strike.

Wiki

• Lab Rota to be removed. (James - done)
• Duplicate project overview to be removed. (James - done)
• Header photo to be changed.
• Add images/text for Next and Previous.
• Reviewed animations - liked, and using.
• Need to change format of Matt's video, and upload. (James)
• All teams need to update lab book.
• Photo date set for Saturday 10th at 10:00am.

Presentation

• Practice Presentation with the Bioinformatics group is set for Friday 23rd October at 1-2pm.
• Still doing a presentation in Sunderland?
• Use Sir Bac Man as first slide.
• Explain the life cycle and how we are changing it.
• Arun has prepared a document.
• Jen and Neil are away in Spain 17-21 October.

Poster

• Jen in to send links to designs.
• Jess' draft has too much small text - more images and talking!
• Need to get back to sponsors to show them what we have achieved.

Could use a complex wheel to point to various points of the poster.