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Team:Osaka/RESULTS
From 2009.igem.org
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<h2>Sensors</h2></div> | <h2>Sensors</h2></div> | ||
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<p>Sensor Experiment 1: Receivers vs AHL<br> | <p>Sensor Experiment 1: Receivers vs AHL<br> | ||
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4. Add AHL to culture.<br> | 4. Add AHL to culture.<br> | ||
5. Measure OD and fluorescence at regular intervals.<br> | 5. Measure OD and fluorescence at regular intervals.<br> | ||
| - | [link to protocol]</p> | + | [link to protocol]</p><br> |
<p>Sensor Experiment 2: Senders vs Receivers<br> | <p>Sensor Experiment 2: Senders vs Receivers<br> | ||
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4. Culture overnight at 37 degrees Celsius in incubator.<br> | 4. Culture overnight at 37 degrees Celsius in incubator.<br> | ||
5. Measure fluorescence.<br> | 5. Measure fluorescence.<br> | ||
| - | [link to protocol] | + | [link to protocol]</p><br> |
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Revision as of 10:40, 21 October 2009
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RESULTS
Sensors
PCR Sequencing
Sensor Experiment 1: Receivers vs AHL
Procedure:
1. Pre-culture cells transformed with parts to be tested in solution medium overnight.
2. Inoculate fresh medium with pre-cultured cells and culture till full growth.
3. Replace culture medium by ultracentrifuging and resuspending cells in fresh medium.
4. Add AHL to culture.
5. Measure OD and fluorescence at regular intervals.
[link to protocol]
Sensor Experiment 2: Senders vs Receivers
Procedure:
1. Pre-culture cells transformed with parts to be tested in solution medium overnight.
2. Pipette fresh medium into microcentrifuge tubes then inoculate with cells from overnight culture.
3. Add AHL for the Receivers-only samples.
4. Culture overnight at 37 degrees Celsius in incubator.
5. Measure fluorescence.
[link to protocol]
Summary
Under construction
Future work
Under construction
