Team:Osaka/RESULTS

From 2009.igem.org

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     <h2>Sensors</h2></div>  
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     <h2>Criteria</h2></div>  
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    <p>PCR Sequencing</p><br>
 
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<p>Sensor Experiment 1: Receivers vs AHL<br>
 
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Procedure:<br>
 
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1. Pre-culture cells transformed with parts to be tested in solution medium overnight.<br>
 
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2. Inoculate fresh medium with pre-cultured cells and culture till full growth.<br>
 
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3. Replace culture medium by ultracentrifuging and resuspending cells in fresh medium.<br>
 
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4. Add AHL to culture.<br>
 
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5. Measure OD and fluorescence at regular intervals.<br>
 
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[link to protocol]</p><br>
 
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<p>Sensor Experiment 2: Senders vs Receivers<br>
 
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Procedure:<br>
 
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1. Pre-culture cells transformed with parts to be tested in solution medium overnight.<br>
 
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2. Pipette fresh medium into microcentrifuge tubes then inoculate with cells from overnight culture.<br>
 
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3. Add AHL for the Receivers-only samples.<br>
 
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4. Culture overnight at 37 degrees Celsius in incubator.<br>
 
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5. Measure fluorescence.<br>
 
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[link to protocol]</p><br>
 
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Revision as of 13:02, 21 October 2009

Home of iGEMOSAKA wiki

RESULTS

Criteria

Summary

Under construction


Future work

Under construction



Retrieved from "http://2009.igem.org/Team:Osaka/RESULTS"