Team:Rice/Notebook
From 2009.igem.org
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- | Eventually this will get formatted differently, but until then... | + | Eventually this will get formatted differently (better), but until then... |
New practices for this year: | New practices for this year: | ||
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5/23/09 | 5/23/09 | ||
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Thomas | Thomas | ||
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Pulled parts: | Pulled parts: | ||
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(Tail switching) | (Tail switching) | ||
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BBa_I719005 - T7 promoter | BBa_I719005 - T7 promoter | ||
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BBa_I719015 - T7 promoter test circuit with GFP | BBa_I719015 - T7 promoter test circuit with GFP | ||
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BBa_B0034 - Strong RBS | BBa_B0034 - Strong RBS | ||
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BBa_B0015 - commonly used strong forward terminator | BBa_B0015 - commonly used strong forward terminator | ||
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BBa_B1006 - new, strong forward terminator | BBa_B1006 - new, strong forward terminator | ||
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(Tet selection) | (Tet selection) | ||
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pSB4K5 - low copy Kan vector | pSB4K5 - low copy Kan vector | ||
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(Part for Dr. Beason) | (Part for Dr. Beason) | ||
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BBa_J45200 | BBa_J45200 | ||
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from the 2009 library plates. | from the 2009 library plates. | ||
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Used 1uL of each to HS transform into XL1 competent cells. | Used 1uL of each to HS transform into XL1 competent cells. |
Latest revision as of 07:13, 25 May 2009
Eventually this will get formatted differently (better), but until then...
New practices for this year:
In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of every part that you pull from the registry.
5/23/09
Thomas
Pulled parts:
(Tail switching)
BBa_I719005 - T7 promoter
BBa_I719015 - T7 promoter test circuit with GFP
BBa_B0034 - Strong RBS
BBa_B0015 - commonly used strong forward terminator
BBa_B1006 - new, strong forward terminator
(Tet selection)
pSB4K5 - low copy Kan vector
(Part for Dr. Beason)
BBa_J45200
from the 2009 library plates.
Used 1uL of each to HS transform into XL1 competent cells.