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| - | <img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div>
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| - | <h1>Biobrick Parts: Rock Paper Scissors</h1>
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| - | <h4> </h4>
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| - | <p> </p>
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| - | <p> </p>
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| - | <p> </p>
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| - | <td colspan="2" align="left" valign="top" bgcolor="#5D5D5D"><h3>For a list of all our parts, please click <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Southampton&Done=1">here</a>.</h3>
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| - | <h3><br />
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| - | To view the parts used in our Game of Life sub-project, click <a href="https://2009.igem.org/Team:Southampton/Parts/GoL">here</a>.</h3>
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| - | <p> </p>
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| - | <td width="108" valign="top" bgcolor="#5D5D5D"><h3><strong>Part Name</strong></h3></td>
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| - | <td width="98" valign="top" bgcolor="#5D5D5D"><h3 align="center"><strong>Type</strong></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3 align="center"><strong>Description</strong></h3></td>
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| - | <td width="164" valign="top" bgcolor="#5D5D5D"><h3 align="center"><strong>Team Favourite?</strong></h3></td>
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| - | <td width="72" valign="top" bgcolor="#5D5D5D"><h3 align="center"><strong>Length</strong></h3></td>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180000">K180000</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Regulatory<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/1/1f/Clip_image002_0004.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Hybrid promoter (trp & lac regulated – tac pR)</strong><br />
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| - | A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">1447</h3></td>
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| - | </tr>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180002">K180002</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>tac pR testing plasmid (GFP)</strong><br />
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| - | Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">2330</h3></td>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180010">K180010</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors – Rock primary plasmid</strong><br />
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| - | Primary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part <a href="http://partsregistry.org/Part:BBa_K180013">K180013</a>.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">3888</h3></td>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180013">K180013</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors – Rock secondary plasmid</strong><br />
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| - | <strong>[rhl pR, LacI generator]</strong><br />
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| - | Secondary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part <a href="http://partsregistry.org/Part:BBa_K180010">K180010</a>.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">1369</h3></td>
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| - | </tr>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180014">K180014</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors – Paper primary plasmid</strong><br />
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| - | Primary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180016">K180016</a>.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">3952</h3></td>
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| - | </tr>
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| - | <tr>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180016">K180016</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors – Paper secondary plasmid</strong><br />
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| - | <strong>[lux pR, LacI generator]</strong><br />
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| - | Secondary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180014">K180014</a>.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">1371</h3></td>
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| - | </tr>
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| - | <tr>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180017">K180017</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors – Scissors primary plasmid</strong><br />
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| - | Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180020">K180020</a>.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">3858</h3></td>
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| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180020">K180020</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
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| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
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| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors – Scissors secondary plasmid </strong><br />
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| - | <strong>[las pR, LacI generator]</strong><br />
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| - | Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180017">K180017</a>.</h3></td>
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| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
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| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">1473</h3></td>
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| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3> </h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3> </h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right"> </h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180003">K180003</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>tac pR testing plasmid (GFP) – basic</strong><br />
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| - | Basic version of <a href="http://partsregistry.org/Part:BBa_K180002">K180002</a>; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence. </h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">2173</h3></td>
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| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180005">K180005</a></h3></td>
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| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Paper – Primary plasmid (part 1)</strong><br />
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| - | <strong>[LuxR generator]</strong><br />
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| - | Part of the paper primary plasmid; constitutively produces LuxR.</h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">979</h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180008">K180008</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Coding<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/9/91/Clip_image007_0000.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>mCherry (rights owned by Clontech)</strong><br />
| |
| - | mCherry fluorescent protein – a derivative of the DsRed fluorescent protein with a short maturation time but lower relative brightness.</h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">708</h3></td>
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| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180009">K180009</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Coding<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/9/91/Clip_image007_0000.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>mBanana (rights owned by Clontech)</strong><br />
| |
| - | mBanana fluorescent protein – a derivative of the DsRed fluorescent protein with a longer maturation time and lower relative brightness than <a href="http://partsregistry.org/Part:BBa_K180008">mCherry</a>.</h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">708</h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180011">K180011</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock – Primary plasmid (part 1)</strong><br />
| |
| - | <strong>[RhlR generator]</strong><br />
| |
| - | Part of the rock primary plasmid; constitutively produces RhlR.</h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">927</h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180012">K180012</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock – Primary plasmid (part 2)</strong><br />
| |
| - | <strong>[tac pR, mCherry and LasI generator]</strong><br />
| |
| - | Part of the rock primary plasmid; generates mCherry and LasI under tac pR regulation.</h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">2953</h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180015">K180015</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Paper – Primary plasmid (part 2)</strong><br />
| |
| - | <strong>[tac pR, GFP and RhlI generator]</strong><br />
| |
| - | Part of the paper primary plasmid; generates GFP and RhlI under tac pR regulation.</h3></td>
| |
| - | <td width="164" bgcolor="#5D5D5D"><h3 align="center"> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">2965</h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180018">K180018</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Scissors – Primary plasmid (part 1)</strong><br />
| |
| - | <strong>[LasR generator]</strong><br />
| |
| - | Part of the scissors primary plasmid; constitutively produces LasR.</h3></td>
| |
| - | <td width="164" valign="top" bgcolor="#5D5D5D"><h3> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">921</h3></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180019">K180019</a></h3></td>
| |
| - | <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
| |
| - | <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
| |
| - | <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Scissors – Primary plasmid (part 2)</strong><br />
| |
| - | <strong>[tac pR, mBanana and LuxI generator]</strong><br />
| |
| - | Part of the scissors primary plasmid; generates mBanana and LuxI under tac pR regulation.</h3></td>
| |
| - | <td width="164" valign="top" bgcolor="#5D5D5D"><h3> </h3></td>
| |
| - | <td width="72" bgcolor="#5D5D5D"><h3 align="right">2929</h3></td>
| |
| - | </tr>
| |
| - | </table><h3> </h3></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </div>
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| - | <h5>| <a href="#">Top</a> |
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| - |
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| - | University of
| |
| - | Southampton iGEM 2009</h5>
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