Team:Southampton/Project/Protocols/Hfpcr

From 2009.igem.org

(Difference between revisions)
m
m
 
(7 intermediate revisions not shown)
Line 7: Line 7:
Wiki</title>
Wiki</title>
<link rel="stylesheet" href="http://www.personal.soton.ac.uk/jeh2v07/css/menu.css" type="text/css"/>
<link rel="stylesheet" href="http://www.personal.soton.ac.uk/jeh2v07/css/menu.css" type="text/css"/>
 +
<link rel="stylesheet" href="http://www.personal.soton.ac.uk/jeh2v07/css/style.css" type="text/css"/>
<style type= "text/css">
<style type= "text/css">
-
#arrowright {
+
/*TOOLBAR*/
 +
#toolbar {
position:absolute;
position:absolute;
-
left:972px;
+
left:-2px;
-
top:300px;
+
top:0px;
-
z-index:10;
+
width:978px;
-
}
+
height:27px;
-
#arrowleft {
+
z-index:100;
-
position:absolute;
+
-
left:-37px;
+
-
top:300px;
+
-
z-index:10;
+
}
}
/*FOOTERPUSH*/
/*FOOTERPUSH*/
#footerpush {
#footerpush {
width:1px;
width:1px;
-
height:1210px;
+
height:1217px;
}
}
html, body {
html, body {
Line 47: Line 45:
position:absolute;
position:absolute;
left:113px;
left:113px;
-
top:1273px;
+
top:1265px;
z-index:150;
z-index:150;
color: #333;
color: #333;
Line 59: Line 57:
z-index:100;
z-index:100;
}
}
-
/*BANNER*/
 
-
#banner {
 
-
position:absolute;
 
-
left:0px;
 
-
top:0px;
 
-
width:975px;
 
-
height:276px;
 
-
z-index:0;
 
-
}
 
-
/*TOOLBAR*/
 
-
#toolbar {
 
-
position:absolute;
 
-
left:3px;
 
-
top:0px;
 
-
width:975px;
 
-
height:27px;
 
-
z-index:100;
 
-
}
 
-
/*BG*/
 
-
#bg {
 
-
position:absolute;
 
-
left:0px;
 
-
top:138px;
 
-
width:975px;
 
-
height:1005px;
 
-
z-index:0;
 
-
}
 
-
#maintop {
 
-
position:absolute;
 
-
left:0px;
 
-
top:138px;
 
-
width:975px;
 
-
height:61px;
 
-
z-index:0;
 
-
}
 
-
/*FLICKRIVER*/
 
-
#flickriver {
 
-
position:absolute;
 
-
left:36px;
 
-
top:1113px;
 
-
width:894px;
 
-
height:134px;
 
-
z-index:0;
 
-
}
 
-
#flickriver2 {
 
-
position:absolute;
 
-
left:480px;
 
-
top:1113px;
 
-
width:894px;
 
-
height:134px;
 
-
z-index:0;
 
-
}
 
-
#blanker {
 
-
position:absolute;
 
-
left:480px;
 
-
top:1113px;
 
-
width:238px;
 
-
height:23px;
 
-
z-index:10;
 
-
}
 
-
#blanker2 {
 
-
position:absolute;
 
-
left:280px;
 
-
top:1224px;
 
-
width:238px;
 
-
height:23px;
 
-
z-index:10;
 
-
}
 
-
/*PROJECT DESCRIPTION*/
 
-
#logobox {
 
-
position:absolute;
 
-
left:90px;
 
-
top:300px;
 
-
width:63px;
 
-
height:63px;
 
-
z-index:10;
 
-
}
 
-
#projectheading {
 
-
position:absolute;
 
-
left:170px;
 
-
top:300px;
 
-
z-index:10;
 
-
}
 
-
#projdesc {
 
-
position:absolute;
 
-
left:25px;
 
-
top:360px;
 
-
width:731px;
 
-
height:273px;
 
-
}
 
-
/*LOGOBOX2*/
 
-
#logobox2 {
 
-
position:absolute;
 
-
left:13px;
 
-
top:695px;
 
-
width:440px;
 
-
height:398px;
 
-
}
 
-
/*UPDATESBOX*/
 
-
#updateheader {
 
-
position:absolute;
 
-
left:795px;
 
-
top:285px;
 
-
}
 
-
#updatedate {
 
-
position:absolute;
 
-
left:895px;
 
-
top:310px;
 
-
}
 
-
#updatecontent {
 
-
position:absolute;
 
-
left:795px;
 
-
top:330px;
 
-
width:164px;
 
-
height:333px
 
-
}
 
-
#updatedate2 {
 
-
position:absolute;
 
-
left:895px;
 
-
top:475px;
 
-
z-index:100;
 
-
}
 
-
#updatecontent2 {
 
-
position:absolute;
 
-
left:795px;
 
-
top:490px;
 
-
width:164px;
 
-
height:333px
 
-
}
 
-
/*LINKBOX*/
 
-
#linkheader {
 
-
position:absolute;
 
-
left:795px;
 
-
top:690px;
 
-
}
 
-
#linksemi {
 
-
position:absolute;
 
-
left:801px;
 
-
top:730px;
 
-
}
 
-
#linklsi {
 
-
position:absolute;
 
-
left:801px;
 
-
top:800px;
 
-
}
 
-
#linksoton {
 
-
position:absolute;
 
-
left:801px;
 
-
top:870px;
 
-
}
 
-
#clustr {
 
-
position:absolute;
 
-
left:795px;
 
-
top:975px;
 
-
}
 
-
/*TEAMBOX*/
 
-
#teamheader {
 
-
position:absolute;
 
-
left:480px;
 
-
top:700px;
 
-
}
 
-
#teampic {
 
-
position:absolute;
 
-
left:472px;
 
-
top:735px;
 
-
width:296px;
 
-
height:164px;
 
-
}
 
-
#teamcaption {
 
-
position:absolute;
 
-
left:480px;
 
-
top:910px;
 
-
width:270px;
 
-
height:100px;
 
-
}
 
-
#facebook {
 
-
position:absolute;
 
-
left:472px;
 
-
top:970px;
 
-
width:97px;
 
-
height:100px;
 
-
}
 
-
#findout {
 
-
position:absolute;
 
-
left:580px;
 
-
top:975px;
 
-
width:185px;
 
-
height:100px;
 
-
}
 
-
#projfind {
 
-
position:absolute;
 
-
left:150px;
 
-
top:920px;
 
-
width:300px;
 
-
height:100px;
 
-
z-index:100;
 
-
}
 
-
/*LAYOUT ENDS*/
 
-
/*HEADINGS BEGIN*/
 
-
h7 {
 
-
font-size: 36px;
 
-
color: #FFFFFF;
 
-
margin-top: 0px;
 
-
margin-bottom: 3px;
 
-
font-weight: normal; font-family: "Book Antiqua",
 
-
Georgia,
 
-
serif;
 
-
}
 
-
h8 {
 
-
font-size: 12px;
 
-
color: #5da8bd;
 
-
margin-top: 0px;
 
-
font-weight: normal;
 
-
font-family: Arial,
 
-
sans-serif;
 
-
}
 
-
h3 {font-size: 13px;
 
-
color: #ebebeb;
 
-
margin-top: 0px;
 
-
font-weight: normal; margin-bottom: 3px;
 
-
font-family: Arial,
 
-
sans-serif;
 
-
}
 
-
h4 {
 
-
font-size: 24px;
 
-
color: #FFFFFF;
 
-
margin-top: 0px;
 
-
font-weight: normal; font-family: "Book Antiqua",Georgia,
 
-
serif;
 
-
}
 
-
h5 {
 
-
font-size: 14px;
 
-
color: #323232;
 
-
margin-top: 0px;
 
-
font-weight: normal; font-family: Arial,
 
-
sans-serif
 
-
}
 
-
h6 {
 
-
font-size: 12px;
 
-
color: #ebebeb;
 
-
margin-top: 0px;
 
-
font-weight: normal; font-family: Arial,
 
-
sans-serif
 
-
}
 
-
/*HEADINGS END*/
 
#bluesubtitle {
#bluesubtitle {
position:absolute;
position:absolute;
Line 318: Line 71:
z-index:100;
z-index:100;
}
}
-
 
</style>
</style>
</head>
</head>
Line 328: Line 80:
<ul class="cssMenu cssMenum">
<ul class="cssMenu cssMenum">
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="#"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<ul class=" cssMenum">
<ul class=" cssMenum">
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
-
         <li class=" cssMenui"><a class="  cssMenui" href="#"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
 +
         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<ul class=" cssMenum">
<ul class=" cssMenum">
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
Line 343: Line 102:
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts">Biobrick Parts</a></li>
+
<li class=" cssMenui"><a class="  cssMenui" href="#">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
-
<li class=" cssMenui"><a class="  cssMenui" href="#"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
<ul class=" cssMenum">
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
 +
</ul></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<ul class=" cssMenum">
<ul class=" cssMenum">
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
+
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling</a></li>
+
-
          
+
            <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling"><span>Modelling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
-
         <li class=" cssMenui"><a class="  cssMenui" href="#"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
<ul class=" cssMenum">
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li>
 +
         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li>
 +
         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li>
 +
        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li>
 +
</ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
 +
             
 +
        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
 +
<ul class=" cssMenum">
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP">Overview</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li>
 +
</ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
 +
   
 +
     
 +
        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<ul class=" cssMenum">
<ul class=" cssMenum">
-
<li class=" cssMenui"><a class="  cssMenui" href="#">Outreach</a></li>
+
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="#">iGEM Experience</a></li>
+
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="#">Political Awareness</a></li>
+
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li>
-
<li class=" cssMenui"><a class="  cssMenui" href="#">Additional</a></li>
+
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
-
    <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li>
+
-
<li class=" cssMenui"><a class="  cssMenui" href="#"><span>Acknowledgments</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
    <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors"><span>Acknowledgments</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<ul class=" cssMenum">
<ul class=" cssMenum">
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li>
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
Line 379: Line 164:
<img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div>
<img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div>
<div id="projectheading">
<div id="projectheading">
-
<h7>Protocol</h7>
+
<h1>Protocol</h1>
</div>
</div>
<div id="bluesubtitle">
<div id="bluesubtitle">
Line 395: Line 180:
</object></div>
</object></div>
-
<div id="arrowright"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/arrowright.png" width="40" height="100"></div>
 
-
<div id="arrowleft"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/arrowleft.png" width="40" height="100"></div>
 
<br>
<br>
</div>
</div>
</div>
</div>
<div id="sufooter">
<div id="sufooter">
-
Home | Project | Biobrick
+
<h5>| <a href="#">Top</a> |&nbsp; &nbsp;&nbsp;&nbsp;
-
Parts | Team | Modelling | Notebook | Sponsors
+
-
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
+
&nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;&nbsp;
University of
University of
-
Southampton iGEM 2009
+
Southampton iGEM 2009</h5>
</div>
</div>
<div id="contfoot">
<div id="contfoot">
<img src="http://www.personal.soton.ac.uk/jeh2v07/images/mainfoot.gif" width="975" height="61" /></div></body>
<img src="http://www.personal.soton.ac.uk/jeh2v07/images/mainfoot.gif" width="975" height="61" /></div></body>
</html>
</html>

Latest revision as of 19:38, 20 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Protocol

High Fidelity PCR

It appears your Web browser is not configured to display PDF files. No worries, just click here to download the PDF file.


| Top |          University of Southampton iGEM 2009