Team:UC Davis/assay for measuring protein localization and activity
From 2009.igem.org
(Difference between revisions)
Line 48: | Line 48: | ||
<div style="text-align: left;"><br> | <div style="text-align: left;"><br> | ||
We will utilize the Western | We will utilize the Western | ||
- | blotting technique to identify and quantify our proteins | + | blotting technique to identify and quantify our proteins. We will also |
- | <br> | + | use a plate reader to quantify fluorescence from GFP and Luciferase |
+ | (our proteins).<br> | ||
<hr style="width: 100%; height: 2px;"><br> | <hr style="width: 100%; height: 2px;"><br> | ||
</div> | </div> |
Revision as of 08:04, 24 September 2009
Assay for
measuring protein localization and activity:
We will utilize the Western blotting technique to identify and quantify our proteins. We will also use a plate reader to quantify fluorescence from GFP and Luciferase (our proteins).
We will utilize the Western blotting technique to identify and quantify our proteins. We will also use a plate reader to quantify fluorescence from GFP and Luciferase (our proteins).