Team:UNC Chapel Hill/2 July 2009

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[[Team:UNC_Chapel_Hill/Notebook|Back]]
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==Notes==
==Notes==
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*Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel.
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*Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel.
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**[http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV]
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***Ladder, Positive Control, RFP, LacY
*Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria:
*Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria:
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**Promoter [http://partsregistry.org/Part:BBa_J23101]
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**[http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1
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**LacI [http://partsregistry.org/Part:BBa_C0012]
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**[http://partsregistry.org/Part:BBa_C0012 LacI] - Plate 2
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**LacI Promoter [http://partsregistry.org/Part:BBa_R0011]
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**[http://partsregistry.org/Part:BBa_R0011 LacI Promoter] - Plate 3
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**pBluescript (positive control) - Plate 4

Latest revision as of 14:46, 9 July 2009

Back


Notes

  • Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel.
    • [http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV]
      • Ladder, Positive Control, RFP, LacY
  • Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria:
    • [http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1
    • [http://partsregistry.org/Part:BBa_C0012 LacI] - Plate 2
    • [http://partsregistry.org/Part:BBa_R0011 LacI Promoter] - Plate 3
    • pBluescript (positive control) - Plate 4