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Team:UNICAMP-Brazil/Protocols/T4 DNA Ligase - Revision history
2024-03-29T05:09:50Z
Revision history for this page on the wiki
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Raissa: New page: {{:Team:UNICAMP-Brazil/inc_topo}} Back to Protocols ==T4 DNA Ligase== - 5X Ligase Reaction Buffer - 4 μl - Insert: Vector Molar Ratio - 3:1 - Vect...
2009-09-29T19:36:01Z
<p>New page: {{:Team:UNICAMP-Brazil/inc_topo}} <a href="/Team:UNICAMP-Brazil/Protocols" title="Team:UNICAMP-Brazil/Protocols">Back to Protocols</a> ==T4 DNA Ligase== - 5X Ligase Reaction Buffer - 4 μl - Insert: Vector Molar Ratio - 3:1 - Vect...</p>
<p><b>New page</b></p><div>{{:Team:UNICAMP-Brazil/inc_topo}}<br />
<br />
[[Team:UNICAMP-Brazil/Protocols|Back to Protocols]]<br />
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==T4 DNA Ligase==<br />
<br />
- 5X Ligase Reaction Buffer - 4 μl <br />
<br />
- Insert: Vector Molar Ratio - 3:1 <br />
<br />
- Vector Ends - 3-30 fmol <br />
<br />
- Insert Ends - 9-90 fmol <br />
<br />
- Total DNA - 0.01-0.1 μg <br />
<br />
- T4 DNA Ligase - 0.1 unit <br />
<br />
- Autoclaved distilled water to 20 uL<br />
<br />
Temperature - 14°C<br />
<br />
Time - 1 h <br />
<br />
''Protocol according to Invitrogen''<br />
<br />
{{:Team:UNICAMP-Brazil/inc_rodape}}</div>
Raissa