Team:UNICAMP-Brazil/Protocols/T4 DNA Ligase

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T4 DNA Ligase

- 5X Ligase Reaction Buffer - 4 μl

- Insert: Vector Molar Ratio - 3:1

- Vector Ends - 3-30 fmol

- Insert Ends - 9-90 fmol

- Total DNA - 0.01-0.1 μg

- T4 DNA Ligase - 0.1 unit

- Autoclaved distilled water to 20 uL

Temperature - 14°C

Time - 1 h

Protocol according to Invitrogen




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