Team:UNIPV-Pavia/Freezer

From 2009.igem.org

(Difference between revisions)

Revision as of 10:59, 14 October 2009

Biobrick/ Ligation Name	Description	Creation Date	Strain	Vector	Quality Control
J23100(J100) replaced by "J23100 Jun4"	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23101(J101) replaced by "J23101 Jun4"	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23102(J102)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23103(J103)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23104(J104)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23105(J105)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23106(J106)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23110(J110)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23112(J112)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23113(J113)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23114(J114)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23116(J116)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23117(J117)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118) replaced by "J23118 Jun4"	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
E0240(GFP3)	GFP protein generator (RBS-GFP-TT)	May 25	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
K131009(E2)	colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)	May 28	TOP10	pSB1AK3	sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB)	colE7 lysis gene	May 28	TOP10	pSB1A2	sequence ok (MIT)
K124017(lysis21)	Phage Lysis Cassette	May 28	TOP10	pSB1AK3	sequence BAD (MIT)
B0015(TT)	Double terminator	May 28	TOP10	pSB1AK3	sequence ok (MIT)
I732017 (RBS-lacZ)	RBS-lacZ	May 28	TOP10	pSB1A2	long part (MIT)
B0030	RBS 0.6	Jun 4	TOP10	pSB1A2	sequence ok (MIT,BMR)
B0031	RBS 0.07	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
B0032	RBS 0.3	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
B0033	RBS 0.01	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
B0034	RBS 1	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
P0440(P04)	RBS-tetR-TT	Jun 4	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
R0040(Ptet)	Ptet	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
T9002	Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT	Jun 4	TOP10	pSB1A3	sequence BAD (MIT) - sequence ok (BMR)
J23100(J100 Jun4)	constitutive promoter	Jun 4	TOP10	J61002(Amp)	sequence ok (MIT,BMR)
J23101(J101 Jun4)	constitutive promoter	Jun 4	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118 Jun4)	constitutive promoter	Jun 4	TOP10	J61002(Amp)	sequence ok (MIT)
A1	J23100(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	TOP10	pSB1A2	length ok (PCR)
A2	J23101(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	TOP10	pSB1A2	length ok (PCR)
A3-1	I732017-B0015 (RBS-lacZ-TT)	Jun 12	TOP10	pSB1AK3	length ok (digestion PstI) sequence ok -long part- (BMR)
A4	P0440-R0040 (RBS-tetR-TT-Ptet)	Jun 11	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A5	A4-A3 (RBS-tetR-TT-Ptet-RBS-lacZ-TT)	Jun 19	TOP10	pSB1AK3	sequence ok -long part- (BMR)
A6	J23100(Jun 4)-A4 (Pcon-RBS-tetR-TT-Ptet)	Jun 19	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
BOL1	J23118-B0034-C0012-B0015 (Pcon-RBS-lacI-TT)	Jun 23	TOP10	pSB1A2	sequence ok (BMR)
A7	J23118(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 25	TOP10	pSB1A2	length ok (PCR)
R0011(Plac)	Plac	Jun 25	TOP10	pSB1A2	sequence ok (MIT)
K112808(ENTERO)	holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)	Jun 25	TOP10	pSB1A2	sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010).
A8	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	TOP10	pSB1A2	NON PURE PLASMID! (digestion E-P) but works
A9	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	TOP10	pSB1A2	NON PURE PLASMID! (digestion E-P) but works
A10 thrown away	K117000-B0015 (celB-TT)	Jun 30	TOP10	pSB1AK3	sequence BAD (BMR)
A8pg	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	TOP10	pSB1A2	length ok (digestion E-P)
A9pg	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	TOP10	pSB1A2	length ok (digestion E-P)
A11-1 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	TOP10	pSB1A2	length ok (PCR) sequence MUTATED
A11-3 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	TOP10	pSB1A2	length ok (PCR)
A11-6 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	TOP10	pSB1A2	length ok (PCR)
F2620 to throw away!	Ptet-RBS-luxR-TT-Plux	Jul 10	TOP10	pSB1A2	sequence BAD (MIT, BMR)
A12-2	J23118(Jun 4)-A4	Jul 16	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A12-3	J23118(Jun 4)-A4	Jul 16	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A14-4 to throw away!	A11-E0240	Jul 16	TOP10	pSB1A2	NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
A14pg to throw away!	A11-E0240	Jul 16	TOP10	pSB1A2	NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
K116002 (PnhaA+GFP)	PnhaA-RBS-GFP-TT	Jul 29	?	pSB1A2	sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter
K112405 (Pcad)	cadAB promoter	Jul 29	?	K112951(Amp+Cm)	sequence ok (BMR)
P0412	RBS-lacI-TT	Jul 29	?	pSB1A2	sequence ok (BMR)
I746902	GFPmut3+his tag generator under Pbad	Jul 29	?	pSB1AK3	sequence ok -long part- (BMR)
I746903	GFPmut3+his tag generator under PT7	Jul 29	?	pSB1AK3	sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same
K101017 to throw away!	MioC promoter	Jul 29	?	pSB1A2	sequence BAD (BMR)
F2620MIT1	Ptet-RBS-luxR-TT-Plux	Jul 29	?	pSB1A2	sequence ok (BMR)
F2620MIT2	Ptet-RBS-luxR-TT-Plux	Jul 29	?	pSB1A2	sequence ok (BMR)
MRGENE1	pdc	Jul 17	TOP10	pMK-RQ (Kan)	antibiotic ok length ok (digestion X-P) sequence ok (Mr Gene)
MRGENE1-2	pdc	Jul 17	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE1-3	pdc	Jul 17	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE2	adhB	Jul 17	TOP10	pMA (Amp)	antibiotic ok length ok (digestion X-P) sequence ok (Mr Gene)
MRGENE2-2	adhB	Jul 17	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
MRGENE2-3	adhB	Jul 17	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
B1-13	B0030-MRGENE1 (RBS-pdc)	Jul 28	TOP10	pSB1A2	length ok (digestion E-P , digestion S-P and digestion E-S) sequence ok (BMR)
B2-5	B0030-MRGENE2 (RBS-adhB)	Jul 23	TOP10	pSB1A2	length ok (PCR and digestion S-P) sequence ok (BMR)
B3-5	B1-B0015 (RBS-pdc-TT)	Jul 31	TOP10	pSB1AK3	length ok+extra band (digestion XhoI-P and digestion X-P) sequence ok (BMR)
B4-2	B2-B0015 (RBS-adhB-TT)	Jul 31	TOP10	pSB1AK3	length ok (digestion XhoI-P) sequence ok (BMR)
B5-3	B1-B4 (RBS-pdc-RBS-adhB-TT)	Aug 12	TOP10	pSB1AK3	length ok+2 extra bands (digestion E-P) sequence ok (BMR)
B6-3	B2-B3 (RBS-adhB-RBS-pdc-TT)	Aug 12	TOP10	pSB1AK3	length ok+2 extra bands (digestion E-P) sequence ok (BMR)
A11-2 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Aug 13	TOP10	pSB1A2	length ok (digestion S-P) sequence MUTATED (BMR)
A11-3	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Aug 13	TOP10	pSB1A2	length ok (digestion S-P) sequence ok (BMR)
A15-1 thrown away by mistake...	F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 14	TOP10	pSB1A2	length ok (digestion) sequence ok (BMR)
A15-2	F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 14	TOP10	pSB1A2	length ok (digestion) sequence WRONG (BMR)
A15-3	F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 14	TOP10	pSB1A2	length ok (digestion) sequence ok (BMR)
B8-5 thrown away!	A4-B6 (RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)	Aug 21	TOP10	pSB1AK3	length maybe ok () sequence WRONG (BMR)
A14-3 to throw away!	A11(-2 Aug13)-E0240 (Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)	Aug 21	TOP10	pSB1A2	length ok (PCR) sequence of A11-2(Aug13) was MUTATED (BMR)
A16-4	A12-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Aug 21	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A17-4 to throw away!	R0011-E0240 (Plac-RBS-GFP-TT)	Aug 28	TOP10	pSB1A2	length ok (PCR) sequence WRONG! ligation failed.
A18-?	A15(-2)-pSB3K3 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 28	TOP10	pSB3K3	still to screen

@@ Line 47: / Line 47: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 55: / Line 54: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 63: / Line 61: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 71: / Line 68: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 79: / Line 75: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 87: / Line 82: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 103: / Line 97: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 111: / Line 104: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 119: / Line 111: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 127: / Line 118: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 135: / Line 125: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 143: / Line 132: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 151: / Line 139: @@
 		<td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td>
                  <td class="prova">May 25</td>
-		<td class="prova">5</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 159: / Line 146: @@
 		<td class="prova">colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)</td>
                  <td class="prova">May 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 167: / Line 153: @@
 		<td class="prova">colE7 lysis gene</td>
                  <td class="prova">May 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 175: / Line 160: @@
 		<td class="prova">Phage Lysis Cassette</td>
                  <td class="prova">May 28</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 183: / Line 167: @@
 		<td class="prova">Double terminator</td>
                  <td class="prova">May 28</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 191: / Line 174: @@
 		<td class="prova">RBS-lacZ</td>
                  <td class="prova">May 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 199: / Line 181: @@
 		<td class="prova">RBS 0.6</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 207: / Line 188: @@
 		<td class="prova">RBS 0.07</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 215: / Line 195: @@
 		<td class="prova">RBS 0.3</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 223: / Line 202: @@
 		<td class="prova">RBS 0.01</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 231: / Line 209: @@
 		<td class="prova">RBS 1</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 239: / Line 216: @@
 		<td class="prova">RBS-tetR-TT</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 247: / Line 223: @@
 		<td class="prova">Ptet</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 255: / Line 230: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A3</td>
@@ Line 263: / Line 237: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 271: / Line 244: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 279: / Line 251: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 287: / Line 258: @@
 		<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 295: / Line 265: @@
 		<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 303: / Line 272: @@
 		<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td>
                  <td class="prova">Jun 12</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 311: / Line 279: @@
 		<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 319: / Line 286: @@
 		<td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td>
                  <td class="prova">Jun 19</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 327: / Line 293: @@
 		<td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td>
                  <td class="prova">Jun 19</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 335: / Line 300: @@
 		<td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td>
                  <td class="prova">Jun 23</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 343: / Line 307: @@
 		<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 351: / Line 314: @@
 		<td class="prova">Plac</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 359: / Line 321: @@
 		<td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 367: / Line 328: @@
 		<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jun 29</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 375: / Line 335: @@
 		<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jun 29</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 383: / Line 342: @@
 		<td class="prova">K117000-B0015<br>(celB-TT)</td>
                  <td class="prova">Jun 30</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 391: / Line 349: @@
 		<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jul 3</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 399: / Line 356: @@
 		<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jul 3</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 407: / Line 363: @@
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 415: / Line 370: @@
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 423: / Line 377: @@
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 431: / Line 384: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 439: / Line 391: @@
 		<td class="prova">J23118(Jun 4)-A4<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 447: / Line 398: @@
 		<td class="prova">J23118(Jun 4)-A4<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 455: / Line 405: @@
 		<td class="prova">A11-E0240<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 463: / Line 412: @@
 		<td class="prova">A11-E0240<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 471: / Line 419: @@
 		<td class="prova">PnhaA-RBS-GFP-TT<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
@@ Line 479: / Line 426: @@
 		<td class="prova">cadAB promoter<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">K112951(Amp+Cm)</td>
@@ Line 487: / Line 433: @@
 		<td class="prova">RBS-lacI-TT<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
@@ Line 495: / Line 440: @@
 		<td class="prova">GFPmut3+his tag generator under Pbad<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 503: / Line 447: @@
 		<td class="prova">GFPmut3+his tag generator under PT7<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 511: / Line 454: @@
 		<td class="prova">MioC promoter<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
@@ Line 519: / Line 461: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">1</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
@@ Line 527: / Line 468: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
 		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
@@ Line 535: / Line 475: @@
 		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
@@ Line 543: / Line 482: @@
 		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
@@ Line 551: / Line 489: @@
 		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
@@ Line 559: / Line 496: @@
 		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
@@ Line 567: / Line 503: @@
 		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
@@ Line 575: / Line 510: @@
 		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
@@ Line 583: / Line 517: @@
 		<td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td>
                  <td class="prova">Jul 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 591: / Line 524: @@
 		<td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td>
                  <td class="prova">Jul 23</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 599: / Line 531: @@
 		<td class="prova">B1-B0015<br>(RBS-pdc-TT)</td>
                  <td class="prova">Jul 31</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 607: / Line 538: @@
 		<td class="prova">B2-B0015<br>(RBS-adhB-TT)</td>
                  <td class="prova">Jul 31</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 615: / Line 545: @@
 		<td class="prova">B1-B4<br>(RBS-pdc-RBS-adhB-TT)</td>
                  <td class="prova">Aug 12</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 623: / Line 552: @@
 		<td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td>
                  <td class="prova">Aug 12</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 631: / Line 559: @@
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Aug 13</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 639: / Line 566: @@
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Aug 13</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 647: / Line 573: @@
 		<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 14</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 655: / Line 580: @@
 		<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 14</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 663: / Line 587: @@
 		<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 14</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 671: / Line 594: @@
 		<td class="prova">A4-B6<br>(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)</td>
                  <td class="prova">Aug 21</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 679: / Line 601: @@
 		<td class="prova">A11(-2 Aug13)-E0240<br>(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)</td>
                  <td class="prova">Aug 21</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 687: / Line 608: @@
 		<td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Aug 21</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 695: / Line 615: @@
 		<td class="prova">R0011-E0240<br>(Plac-RBS-GFP-TT)</td>
                  <td class="prova">Aug 28</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 703: / Line 622: @@
 		<td class="prova">A15(-2)-pSB3K3<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 28</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB3K3</td>