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Team:UNIPV-Pavia/Methods Materials/Evaporation
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| - | == <html><font class="dayw_style">The "frame effect" on the plate</html> == | + | == <html><font class="dayw_style">The "frame effect" on the plate</font></html> == |
In the first experiments we did not use the lid on the microplate and we find out that the wells were subjected at a non uniform evaporation phenomenon, if the plate was left in incubation at 37°C inside the instrument. | In the first experiments we did not use the lid on the microplate and we find out that the wells were subjected at a non uniform evaporation phenomenon, if the plate was left in incubation at 37°C inside the instrument. | ||
In particular we observed that the evaporation was larger in the wells on the frame of the plate. | In particular we observed that the evaporation was larger in the wells on the frame of the plate. | ||
| - | We tried to correct this phenomen dispensing water through the instrument injector, but we did not find a satisfactory dispensing protocol, as you can see in the figure below, which shows the final volumes in the wells after a 24 hours incubation. The initial volume was 200 (LB medium) and others 110 were added during the incubation. | + | We tried to correct this phenomen dispensing water through the instrument injector, but we did not find a satisfactory dispensing protocol, as you can see in the figure below, which shows the final volumes in the wells after a 24 hours incubation. The initial volume was 200 μl (LB medium) and others 110 μl were added during the incubation. |
| + | You can find more informations about the experiments at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.3, 10/06/09. | ||
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| + | [[Image:Unipv_evaporation_1.jpg| 500px|Evaporation phenomenon|thumb|center|Evaporation phenomenon without lid, LB]] | ||
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Revision as of 23:44, 12 October 2009
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Measurements - Evaporation |
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The "frame effect" on the plateIn the first experiments we did not use the lid on the microplate and we find out that the wells were subjected at a non uniform evaporation phenomenon, if the plate was left in incubation at 37°C inside the instrument. In particular we observed that the evaporation was larger in the wells on the frame of the plate. We tried to correct this phenomen dispensing water through the instrument injector, but we did not find a satisfactory dispensing protocol, as you can see in the figure below, which shows the final volumes in the wells after a 24 hours incubation. The initial volume was 200 μl (LB medium) and others 110 μl were added during the incubation. You can find more informations about the experiments at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.3, 10/06/09.
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