Team:UNIPV-Pavia/Methods Materials/GelExtraction

From 2009.igem.org

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<h1>DNA gel extraction</h1>
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=='''DNA gel extraction'''==
''(Estimated time: 1 hour and 30 min)''
''(Estimated time: 1 hour and 30 min)''
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'''Materials needed:'''
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*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
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*'''Agarose gel with well-separated, sharp bands'''
*'''Agarose gel with well-separated, sharp bands'''
*'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut)
*'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut)
*'''Roche Agarose Gel DNA Extraction Kit'''
*'''Roche Agarose Gel DNA Extraction Kit'''
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*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
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Revision as of 12:13, 30 June 2009

EthanolPVanimation.gif



Protocols

DNA gel extraction

(Estimated time: 1 hour and 30 min)










  • Agarose gel with well-separated, sharp bands
  • Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut)
  • Roche Agarose Gel DNA Extraction Kit