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Team:UNIPV-Pavia/Methods Materials/Measurement
From 2009.igem.org
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<font face="Pristina" size="50" ><b>Measurements</b></font> | <font face="Pristina" size="50" ><b>Measurements</b></font> | ||
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A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in [https://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/TECAN#TECAN_Infinite_F200 Instruments], to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations. | A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in [https://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/TECAN#TECAN_Infinite_F200 Instruments], to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations. | ||
| - | The results of | + | The results of this experimental setup, grouped by arguments, are described here. If you need more information about a single experiment, you can find the detailed protocol at <html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/" target="_blank">Download Protocols</a></html>. |
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*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and water dispensation| Absorbance and water dispensation]] | *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and water dispensation| Absorbance and water dispensation]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and volume|Absorbance and volume]] | *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and volume|Absorbance and volume]] | ||
| - | *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and dilutions in liquid | + | *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and dilutions in liquid growth medium|Absorbance and dilutions in liquid growth medium]] |
| + | *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Different protocols for different growth curves|Different protocols for different growth curves]] | ||
| + | *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Growth ambients in comparison|Growth ambients in comparison]] | ||
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'''FLUORESCENCE''': | '''FLUORESCENCE''': | ||
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*[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in water|Fluorescence and dilutions in water]] | *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in water|Fluorescence and dilutions in water]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and volume|Fluorescence and volume]] | *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and volume|Fluorescence and volume]] | ||
| - | *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in liquid | + | *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in liquid growth medium|Fluorescence and dilutions in liquid growth medium]] |
*[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Temporal evolution of fluorescence measurements|Temporal evolution of fluorescence measurements]] | *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Temporal evolution of fluorescence measurements|Temporal evolution of fluorescence measurements]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Bleaching and autofluorescence|Bleaching and autofluorescence]] | *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Bleaching and autofluorescence|Bleaching and autofluorescence]] | ||
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| + | CONCLUSIONS: EXPERIMENTAL SET UP AND MEASUREMENT METHODS | ||
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| + | • In long experiments do not use the wells on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate;<br><br> | ||
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| + | • in long experiments cover the plate with the lid to limit the evaporation phenomenon;<br><br> | ||
| + | • use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well;<br><br> | ||
| - | < | + | • do not dispense water if you perform fluorescence measurements;<br><br> |
| - | + | ||
| + | • work with volumes not larger than 200 μl and prefer the M9 medium if you perform fluorescence measurements;<br><br> | ||
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| + | • in long experiments, if you perform fluorescence measurements on fluorescent cultures, always subtract the blank value individually on every sampling to correct the descending evolution of the fluorescence during the incubation. | ||
| + | <br><br> | ||
</font> | </font> | ||
</td> | </td> | ||
Latest revision as of 16:06, 19 October 2009
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Measurements |
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A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations. The results of this experimental setup, grouped by arguments, are described here. If you need more information about a single experiment, you can find the detailed protocol at Download Protocols.
ABSORBANCE:
EVAPORATION:
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