Team:USTC/Parts

From 2009.igem.org

USTC
Home Team Project Modeling Parts Standard & Protocol Software Tool Human Practice Notebook

Team:USTC/Parts

Contents

To get the information of Parts, pleast visit [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=USTC USTC 2009 Parts]


This year we have submitted 167 BioBrick parts, some of which were well characterized by a lot of measurements and analysised by modeling.

Here we seperated all the parts into different sets to describe according to our progress.
Assembly Road Map_1: Milestone1.
Assembly Road Map_2: Milestone2.

1

Sent? Works? Name Type Description Designer Length
SentWorksRegulatorypLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPSDanqian Liu, Chao Li, Hao Jiang72

2

Sent? Works? Name Type Description Designer Length
SentWorksReporterpLux/Tet(K176000)(LuxR+,TetR-)->RBS+GFP+TChao Li,Danqian Liu,Hao Jiang955

3

Sent? Works? Name Type Description Designer Length
SentWorksSignallingTetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Chao Li, Hao Jiang1059
SentWorksSignallingTetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1059
SentWorksSignallingTetR repressible AHL->PoPS Receiver (Medium LuxR): pCon 0.15->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1059
SentWorksSignallingTetR repressible AHL->PoPS Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1059

4

Sent? Works? Name Type Description Designer Length
UnsentIssuesTranslational_UnitPoPS->RBS+ccdB->PoPSZongxiao He, Hao Jiang324
SentIssuesTranslational_UnitPoPS->RBS+lacZalpha-ccdB->PoPSZongxiao He, Hao Jiang498
SentIssuesGeneratorPoPS->RBS+ccdB+TZongxiao He, Hao Jiang461
SentIssuesGeneratorPoPS->RBS+lacZalpha-ccdB+TZongxiao He, Hao Jiang635
SentIssuesTranslational_UnitPoPS->RBS(weak)+ccdB->PoPSZongxiao He, Hao Jiang326
SentIssuesTranslational_UnitPoPS->RBS(weak)+lacZalpha-ccdB->PoPSZongxiao He, Hao Jiang500
SentIssuesGeneratorPoPS->RBS(weak)+ccdB+TZongxiao He, Hao Jiang463
SentIssuesGeneratorPoPS->RBS(weak)+lacZalpha-ccdB+TZongxiao He, Hao Jiang637
SentIssuesCodingccdB-LVA coding sequenceDanqian Liu, Zongxiao He, Hao Jiang342
UnsentIssuesCodinglacZalpha-ccdB-LVA coding sequenceDanqian Liu, Zongxiao He, Hao Jiang513
SentIssuesTranslational_UnitPoPS->RBS+ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang360
SentIssuesTranslational_UnitPoPS->RBS+lacZalpha-ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang531
UnsentIssuesTranslational_UnitPoPS->RBS(weak)+ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang362
SentIssuesTranslational_UnitPoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang533
SentIssuesGeneratorPoPS->RBS+ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang497
SentIssuesGeneratorPoPS->RBS+lacZalpha-ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang668
UnsentIssuesGeneratorPoPS->RBS(weak)+ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang499
SentIssuesGeneratorPoPS->RBS(weak)+lacZalpha-ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang670

5

Sent? Works? Name Type Description Designer Length
SentWorksRegulatoryconstitutive promoter family member J23115 actual sequence (pCon 0.15)Hao Jiang, Danqian Liu, Chao Li35
SentWorksRegulatoryconstitutive promoter family member J23107 actual sequence (pCon 0.36)Hao Jiang, Danqian Liu, Chao Li35

6

Sent? Works? Name Type Description Designer Length
SentWorksReporterpCon max(J23119)->RBS+GFP+TChao Li,Danqian Liu,Hao Jiang918
SentWorksReporterpCon 1.00(J23100)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.70(J23101)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.36(K176009)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.15(K176008)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.04(J23109)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.01(J23103)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCI(R0051)(lambda CI-)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang932

7

Sent? Works? Name Type Description Designer Length
UnsentFailsGeneratorpCon max(J23119)->RBS+luxR+TChao Li,Danqian Liu,Hao Jiang979
SentWorksGeneratorpCon 0.70(J23101)->RBS+luxR+TDanqian Liu, Chao Li, Hao Jiang979

8

Sent? Works? Name Type Description Designer Length
SentNoneSignallingpCon max(J23119)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentNoneSignallingpCon 1.00(J23100)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.70(J23101)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.36(K176009)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.15(K176008)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.04(J23109)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.01(J23103)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841

9

Sent? Works? Name Type Description Designer Length
SentWorksSignallingTetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Chao Li, Hao Jiang1942
SentWorksSignallingTetR repressible AHL->GFP Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang1942
SentWorksSignallingTetR repressible AHL->GFP Receiver (Medium LuxR): pCon 0.15->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang1942
SentWorksSignallingTetR repressible AHL->GFP Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang1942

10

Sent? Works? Name Type Description Designer Length
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdBZongxiao He, Hao Jiang1528
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdBZongxiao He, Hao Jiang1702
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Zongxiao He, Hao Jiang1530
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Zongxiao He, Hao Jiang1704
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1530
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1704
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang1564
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang1735
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1566
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1737
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1530
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1704
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1530
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1704

11

Sent? Works? Name Type Description Designer Length
SentWorksGeneratorPoPS->RBS+tetR(no LVA)+TChao Li,Danqian Liu,Hao Jiang782
SentWorksGeneratorPoPS->RBS+tetR-LVA+TChao Li,Danqian Liu,Hao Jiang840

12

Sent? Works? Name Type Description Designer Length
SentWorksDeviceRepressible AHL->GFP Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->GFPChao Li, Danqian Liu, Hao Jiang2732
SentWorksDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFPChao Li, Danqian Liu, Hao Jiang2790
SentWorksDeviceRepressible AHL->PoPS Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->PoPSChao Li, Danqian Liu, Hao Jiang1849
SentWorksDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPSChao Li, Danqian Liu, Hao Jiang1907
UnsentFailsDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1907
SentWorksDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2790
UnsentFailsDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1907
UnsentFailsDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2790
UnsentFailsDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1907
UnsentFailsDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2790

13

Sent? Works? Name Type Description Designer Length
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1892
SentNoneDeviceaTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1950
SentNoneDeviceaTc&AHL->GFP: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2775
SentNoneDeviceaTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.36->tetR+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1892
UnsentWorksDeviceaTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1950
SentNoneDeviceaTc&AHL->GFP: pCon 0.36->tetR+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2775
SentWorksDeviceaTc&AHL->GFP: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.15->tetR+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1892
UnsentWorksDeviceaTc&AHL->PoPS: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1950
SentNoneDeviceaTc&AHL->GFP: pCon 0.15->tetR+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2775
SentWorksDeviceaTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2833
UnsentWorksDeviceaTc&AHL->PoPS: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1892
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPSZongxiao He, Hao Jiang1950
SentWorksDeviceaTc&AHL->GFP: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2775
UnsentNoneDeviceaTc&AHL->GFP: pCon 0.01->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFPZongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
SentWorksDeviceaTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
SentNoneDeviceaTc&AHL->GFP: pCon 0.36->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.15->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
SentWorksDeviceaTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.04->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
SentNoneDeviceaTc&AHL->GFP: pCon 0.04->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
SentWorksDeviceaTc&AHL->GFP: pCon 0.01->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
UnsentNoneDeviceaTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
UnsentNoneDeviceaTc&AHL->GFP: pCon 0.36->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.15->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
UnsentNoneDeviceaTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.04->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
UnsentNoneDeviceaTc&AHL->GFP: pCon 0.04->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833
UnsentNoneDeviceaTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1950
UnsentNoneDeviceaTc&AHL->GFP: pCon 0.01->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2833

14

Sent? Works? Name Type Description Designer Length
UnsentWorksDevicePoPS*cell density->PoPS: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1865
SentWorksDevicecell density->PoPS: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
SentWorksDevicecell density->PoPS: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
SentWorksDevicecell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
SentWorksDevicecell density->PoPS: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
SentWorksDevicecell density->PoPS: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
SentWorksDevicecell density->PoPS: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
SentWorksDevicecell density->PoPS: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Hao Jiang1908
UnsentIssuesDeviceCell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2510
SentIssuesDeviceCell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
UnsentIssuesDeviceCell density controller: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
SentIssuesDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
SentIssuesDeviceCell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
SentIssuesDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
SentIssuesDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
SentIssuesDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Hao Jiang2553
UnsentIssuesDeviceCell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2336
SentIssuesDeviceCell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDeviceCell density controller: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
SentIssuesDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDeviceCell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
SentIssuesDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
SentIssuesDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDevicecell density->PoPS: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1908
UnsentIssuesDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2553
UnsentIssuesDevicecell density->PoPS: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1908
UnsentIssuesDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2553
UnsentIssuesDevicecell density->PoPS: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1908
UnsentIssuesDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
UnsentIssuesDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2553
FailsDevicecell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1908
FailsDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2379
FailsDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2553
FailsDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2413
FailsDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2584
FailsDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2415
FailsDeviceCell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2586
FailsDeviceCell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2413
FailsDeviceCell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2584
FailsDeviceCell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2415
FailsDeviceCell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2586
FailsDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2413
FailsDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2584
FailsDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2415
FailsDeviceCell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2586
FailsDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2413
FailsDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2584
FailsDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2415
FailsDeviceCell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2586
FailsDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2413
FailsDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang2584
FailsDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2415
FailsDeviceCell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang2586
SentWorksDevicecell density->GFP: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2791
SentWorksDevicecell density->GFP: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2791
SentWorksDevicecell density->GFP: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2791
SentWorksDevicecell density->GFP: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2791
SentWorksDevicecell density->GFP: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2791

15

These are what we called [barcodes], through which we can identify a definite kind of E.coli quickly.

Sent? Works? Name Type Description Designer Length
SentWorksDNAReverse primer VR3 (K176047) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR4 (K176048) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR5 (K176049) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR6 (K176050) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR7 (K176051) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR8 (K176052) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR9 (K176053) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR10 (K176054) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR11 (K176055) binding siteJiayi Dou, Hao Jiang20
SentWorksDNAReverse primer VR12 (K176056) binding siteJiayi Dou, Hao Jiang20

16

Sent? Works? Name Type Description Designer Length
SentWorksRegulatoryconstitutive promoter family member J23119 (pCon max) with primer VR10 siteJiayi Dou, Hao Jiang63
SentWorksRegulatoryconstitutive promoter family member J23100 (pCon 1.00) with primer VR3 siteJiayi Dou, Hao Jiang63
SentWorksRegulatoryconstitutive promoter family member J23101 (pCon 0.70) with primer VR4 siteJiayi Dou, Hao Jiang63
SentWorksRegulatoryconstitutive promoter family member K176009 (pCon 0.36) with primer VR7 siteJiayi Dou, Hao Jiang63
SentWorksRegulatoryconstitutive promoter family member K176008 (pCon 0.15) with primer VR9 siteJiayi Dou, Hao Jiang63
SentWorksRegulatoryconstitutive promoter family member J23109 (pCon 0.04) with primer VR8 siteJiayi Dou, Hao Jiang63
SentWorksRegulatoryconstitutive promoter family member J23103 (pCon 0.01) with primer VR5 siteJiayi Dou, Hao Jiang63

17

Sent? Works? Name Type Description Designer Length
SentNoneDeviceRepressible cell density controller: PoPS->tetR-LVA->K176096Danqian Liu, Zongxiao He, Hao Jiang3401
SentNoneDeviceRepressible cell density controller: PoPS->tetR-LVA->K176098Danqian Liu, Zongxiao He, Hao Jiang3401
SentNoneDeviceRepressible cell density controller: PoPS->tetR-LVA->K176095Danqian Liu, Zongxiao He, Hao Jiang3401
SentNoneDeviceRepressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPSChao Li, Hao Jiang2756

18

Sent? Works? Name Type Description Designer Length
SentNoneTranslational_UnitPoPS->RBS+GFP->RBS+luxI-LVA->PoPSDanqian Liu, Hao Jiang1407
SentNoneTranslational_UnitPoPS->RBS+mRFP->RBS+luxI-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang1393
UnsentNoneDeviceHigh input score automatic selector: PoPS->luxI-LVA->K176226Danqian Liu, Zongxiao He, Hao Jiang4070
SentIssuesDeviceHigh input score automatic selector (with reporter): PoPS->GFP->K176229Danqian Liu, Zongxiao He, Hao Jiang4816
UnsentNoneDeviceHigh input score automatic selector: PoPS->luxI-LVA->K176227Danqian Liu, Hao Jiang4070
UnsentNoneDeviceHigh input score automatic selector: PoPS->luxI-LVA->K176228Danqian Liu, Hao Jiang4070
UnsentNoneDeviceHigh input score automatic selector without lethal device: PoPS->luxI-LVA->K176237->PoPSChao Li, Hao Jiang3425
UnsentNoneDeviceHigh input score automatic selector (with reporter): PoPS->GFP->K176238Danqian Liu, Hao Jiang4816
SentNoneDeviceHigh input score automatic selector (with reporter): PoPS->GFP->K176239Danqian Liu, Hao Jiang4816
SentNoneDeviceHigh input score automatic selector (with reporter): PoPS->mRFP->K176229Danqian Liu, Hao Jiang4802
SentNoneDeviceHigh input score automatic selector without lethal device (with reporter): PoPS->mRFP->K176240->PoPSChao Li, Hao Jiang4157

19

Sent? Works? Name Type Description Designer Length
SentNoneDeviceFor characterization of K176226: VR7+pCon 0.36->K176226Danqian Liu, Zongxiao He, Hao Jiang3472

20

Sent? Works? Name Type Description Designer Length
UnsentNoneDeviceFor characterization of K176230: VR4+pCon 0.70->K176230Danqian Liu, Zongxiao He, Hao Jiang4887
SentNoneDeviceFor characterization of K176230: VR7+pCon 0.36->K176230Danqian Liu, Zongxiao He, Hao Jiang4887
SentNoneDeviceFor characterization of K176230: VR9+pCon 0.15->K176230Danqian Liu, Zongxiao He, Hao Jiang4887
UnsentNoneDeviceFor characterization of K176230: VR8+pCon 0.04->K176230Danqian Liu, Zongxiao He, Hao Jiang4887
SentNoneDeviceFor characterization of K176230: VR5+pCon 0.01->K176230Danqian Liu, Zongxiao He, Hao Jiang4887
SentNoneDeviceFor characterization of K176240: VR7+pCon 0.36->K176240->PoPSChao Li, Hao Jiang3496
SentNoneDeviceFor characterization of K176240: VR9+pCon 0.15->K176240->PoPSChao Li, Hao Jiang3496
SentNoneDeviceFor characterization of K176240: VR5+pCon 0.01->K176240->PoPSChao Li, Hao Jiang3496

21-27

Sent? Works? Name Type Description Designer Length
SentWorksReporterCI Inverter + GFP Reporter: PoPS->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1870
SentWorksReporterCI Inverter + GFP Reporter: VR10+pCon max->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentWorksReporterCI Inverter + GFP Reporter: VR3+pCon 1.00->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentWorksReporterCI Inverter + GFP Reporter: VR4+pCon 0.70->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentWorksReporterCI Inverter + GFP Reporter: VR7+pCon 0.36->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentWorksReporterCI Inverter + GFP Reporter: VR9+pCon 0.15->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentWorksReporterCI Inverter + GFP Reporter: VR8+pCon 0.04->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentWorksReporterCI Inverter + GFP Reporter: VR5+pCon 0.01->cI-LVA+pCI->GFPDanqian Liu, Hao Jiang1941
SentNoneSignallingCI Inverter + luxI-LVA: PoPS->cI-LVA+pCI->luxI-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang1656
SentNoneCodingActivation-Induced (Cytidine) Deaminase (standard version of K103001)Danqian Liu, Zongxiao He, Hao Jiang600
SentNoneTranslational_UnitPoPS->RBS(weak)+AID->PoPSDanqian Liu, Zongxiao He, Hao Jiang620
SentNoneSignallingPoPS->GFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang2402
SentNoneDevicePoPS->AID(week RBS)->GFP->CI Inverter (cI-LVA+pCI)->luxI-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang3030
SentNoneGeneratorPoPS->RBS(weak)+AID+TDanqian Liu, Zongxiao He, Hao Jiang757
SentNoneSignallingPoPS->mRFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang2388
SentNoneDevicePoPS->AID(week RBS)->mRFP->CI Inverter (cI-LVA+pCI)->luxI-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang3016
SentNoneDeviceLow input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229Danqian Liu, Hao Jiang5797
SentNoneDeviceLow input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240Chao Li, Hao Jiang5152