Team:Valencia/Notebook/July

From 2009.igem.org

(Difference between revisions)
Line 161: Line 161:
- We prepared our first [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium.]]
- We prepared our first [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium.]]
-
- We calibrated our calorimeters with hot water.
+
<br><br><br><br><br><br>
-
=== July 4th ===
+
<center><html>
-
- We prepared some plates for inoculum. They were stored a the 28ºC stove.
+
<div style=" width:50%;">
-
 
+
<h2>
-
=== July 7th ===
+
Back to<a href="https://2008.igem.org/Team:Valencia/Notebook/June"><font color="#047DB5"> June </font></a>&nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;July &nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;Go to <a href="https://2008.igem.org/Team:Valencia/Notebook/August"><font color="#047DB5"> August </font></a></h2>
-
- We prepared some inoculum in Erlenmeyer flasks for our experiment the following day. We kept them overnight in a 30ºC shaking stove.
+
</div>
-
 
+
</html></center>
-
=== July 8th ===
+
-
- The experiment consisted on: <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains <br>
+
-
We left it overnight, but the air conditioning went off and the room temperature rose. 
+
-
 
+
-
===July 9th===
+
-
- We characterized culture growth in our calorimeters by measuring DO, with just one strain.<br>
+
-
 
+
-
===July 10th===
+
-
- Trying to see if there is basal rate of UCP1. Experiment: <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Calorimeters containing: water, medium, UCP-, Gly 175Δ.
+
-
 
+
-
===July 11th===
+
-
- It looks like our ''Saccharomyces cerevisiae'' mutant strain has a basal rate of UCP1... We repeated the experiment to be sure.
+
-
 
+
-
===July 12th===
+
-
- We obtained the same results.
+
-
 
+
-
===July 14th===
+
-
- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.
+
-
 
+
-
===July 15th===
+
-
- We prepared inoculum in Erlenmeyer flasks. We kept them overnight in a 30ºC shaking stove.
+
-
+
-
===July 16th===
+
-
- Trying to find out which is the best moment for activating UCP1.... The glucose in the medium must be consumed by the induction time. DO at the beginning of the experiment was 0.05. <br>
+
-
Experiment: <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]] <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Galactose activation. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; One strain. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Activation at two, three and four hours after inoculating. D.O. was measured at each time.<br>
+
-
 
+
-
===July 17th===
+
-
- Our lab electricity went off during the night. How many experiments will have it ruined?
+
-
 
+
-
===July 18th===
+
-
- We prepared lots of plates with our [[Team:Valencia/Notebook/Protocols#SD Medium |SD medium]]...Unfortunately, we forgot to add glucose.
+
-
 
+
-
- We carried out the pilot experiment: <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SP liquid medium |SP medium]]. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Galactose activation at 3 hours (approximate DO 0.15). <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains. <br>
+
-
We see weird oscillations.
+
-
 
+
-
===July 20th===
+
-
- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.
+
-
 
+
-
===July 21st===
+
-
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] from July 18th again. Weird results, we need to repeat it.
+
-
 
+
-
===July 22nd===
+
-
- We repaired our calorimeters. They were the reason for yesterday weird results.
+
-
 
+
-
===July 23rd===
+
-
- We repeated the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] from July 18th.
+
-
 
+
-
===July 24th===
+
-
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste... They wanted to find out if the oscillations would stop if they covered the thermocouples with different materials they found in the contiguuos labs, or immerse them in the liquid. <br>
+
-
Meri, who is in charged of the lab, wasn't very happy the following day...
+
-
 
+
-
===July 25th===
+
-
- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.
+
-
 
+
-
===July 26th===
+
-
- One of our calorimeters wasn't working properly.
+
-
 
+
-
===July 28th===
+
-
- We repaired the calorimeter and calibrated once again.
+
-
 
+
-
===July 29th===
+
-
- Our cultures haven't grown enough for the experiment. Temperature and medium were all right, we'll have to wait...
+
-
 
+
-
===July 30th===
+
-
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]], same as July 18th. Weird results...
+
-
 
+
-
===July 31st===
+
-
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] once again.
+

Revision as of 15:51, 6 August 2009


Home Team Project Human Practices Notebook News

April
Mo Tu We Th Fr Sa Su
30 31  1 2  3  4  5
 6 7  8 9 10 11 12
13 14 15 16 17 18 19
2021 22 23 24 25 26
2728 29  30  1  2  3
May
Mo Tu We Th Fr Sa Su
27 28 29 30  1  2  3
 4 5  6 7  8  9 10
1112 13 14 15 16 17
1819 20 21 22 23 24
2526 27  28 29 30 31
June
Mo Tu We Th Fr Sa Su
 1 2  3   4  5  6  7
 8  9 10  11 12 13 14
1516 17  18 19 20 21
2223 24  25 26 27 28
2930  1   2  3  4  5
July
Mo Tu We Th Fr Sa Su
29 30  1  2  3  4  5
 6 7  8   9 10 11 12
13 14 15  16 17 18 19
2021 22  23 24 25 26
2728 29  30 31   1  2
August
Mo Tu We Th Fr Sa Su
27 28 29  30 31  1  2
 3  4  5   6  7  8  9
10 11]] 12  13 14 15 16
17 18 19  20 21 22 23
24 25 26  27 28 29 30
311 2 3 4 5 6
September
Mo Tu We Th Fr Sa Su
31 1  2   3  4  5  6
 7 8  9  10 11 12 13
14 15 16  17 18 19 20
2122 23  24 25 26 27
2829 30  1 2 3 4
October
Mo Tu We Th Fr Sa Su
28 29 30    1  2  3  4
 5 6  7   8 9 10 11
1213 14  15 16 17 18
1920 21  22 23 24 25
26 27 28  29 30 31  1
November
Mo Tu We Th Fr Sa Su
26 27 28  29 30 31  1
 2 3  4   5  6  7  8
 910 11  12 13 14 15
1617 18  19 20 21 22
23 24 25  26 27 28 29
30  1  2  3  4  5  6

July 1st

- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6 Eduardo Rial]  :
      Negative control, UCP -.
      Strain containing UCP1, UCP +.
      Mutant stains over expressing UCP1, Gly 76Δ and Gly 175Δ.


- We calibrated our calorimeters with hot water.

July 2nd

- We prepared our first SD medium.







Back to June    |    July    |    Go to August