Team:Valencia/Parts

From 2009.igem.org

(Difference between revisions)
(Our BioBricks)
Line 1: Line 1:
{{Template:Valencia09iGEM23}}
{{Template:Valencia09iGEM23}}
-
<div align="justify" style="position:relative; top:-5px; left:30px; width:700px; color:black; font-size:10pt; font-family: Verdana">
+
<div align="justify" style="position:relative; top:-250px; left:10px; width:700px; color:black; font-size:10pt; font-family: Verdana">
<br>
<br>

Revision as of 22:18, 21 October 2009



Our BioBricks


We have contributed to the Registry with a set of parts, but one of them outstands all the others: aequorin. This reporter protein needs a prosthetic group, coelenterazine, and citoplasmic Ca2+ to work. This means that light is only emitted whenever the researcher uses coelenterazine and whenever there is a media full of Ca2+. There are some works that use aequorin to sense Ca2+, but we have tamed the protein to emit light whenever we want with the activation through electric current.

A thorough characterization has been made and can be found either at the corresponding page in this wiki or at the Registry.

Additionally, we have used a collection of knock outs in order to have genetic negative control that help us confirm that the light effect is not an artifact.

Aequorin.GIF


a 3D model of aequorin, our outstanding BioBrick contribution to the Registry.