(Difference between revisions)
Line 8: Line 8:
<div align="justify" style="position:relative; top:-265px; left:10px; width:700px; color:black; font-size:10pt; font-family: Verdana">
<div align="justify" style="position:relative; margin-top:-250px; margin-left:190px; width:700px; color:black; font-size:10pt; font-family: Verdana">

Revision as of 23:24, 21 October 2009

Our BioBricks

We have contributed to the Registry with a set of parts, but one of them outstands all the others: aequorin. This reporter protein needs a prosthetic group, coelenterazine, and citoplasmic Ca2+ to work. This means that light is only emitted whenever the researcher uses coelenterazine and whenever there is a media full of Ca2+. There are some works that use aequorin to sense Ca2+, but we have tamed the protein to emit light whenever we want with the activation through electric current.

A thorough characterization has been made and can be found either at the corresponding page in this wiki or at the Registry.

Additionally, we have used a collection of knock outs in order to have genetic negative control that help us confirm that the light effect is not an artifact.


a 3D model of aequorin, our outstanding BioBrick contribution to the Registry.