Team:Valencia/Parts/Characterization

From 2009.igem.org

(Difference between revisions)
(General information)
Line 9: Line 9:
[[Image:aequorin.GIF|200px|center]]
[[Image:aequorin.GIF|200px|center]]
-
It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but if it binds to its cofactor coelenterazine, in presence of Ca2+, aequorin emits light.
+
It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but when it binds to its cofactor coelenterazine, in presence of Ca2+, aequorin emits light.
==Sequence==
==Sequence==

Revision as of 11:57, 9 October 2009














General information

AEQ is a gene which encodes for aequorin, our luminiscent protein.


Aequorin.GIF

It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but when it binds to its cofactor coelenterazine, in presence of Ca2+, aequorin emits light.

Sequence

Aequorin sequence is (primer binding sites are underlined in green):

Aeqseqval.JPG