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Team:Virginia/10 June 2009
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ThaddeusWebb (Talk | contribs) (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *No colonies from plasmid transformations ==Today== *Re-transformed plasmids *Prepared -20 glycerol stocks of knockouts JW3469 and JW3...) |
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==Overnight Results== | ==Overnight Results== | ||
| - | * | + | *Only saw growth in pSB1C3 broth |
| + | *Have colonies on A09-1(2) plate | ||
==Today== | ==Today== | ||
| - | *Re-transformed | + | *Made 5ml overnight broths from 3 colonies on A09-1(2) |
| + | *Miniprepped pSB1C3 | ||
| + | **18.4 ng/ul | ||
| + | *Re-transformed vector DNA in DB3.1 | ||
| + | **included control transformation in DH5a, should yield no colonies | ||
*Prepared -20 glycerol stocks of knockouts JW3469 and JW3470. | *Prepared -20 glycerol stocks of knockouts JW3469 and JW3470. | ||
Latest revision as of 18:30, 10 June 2009
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Overnight Results
- Only saw growth in pSB1C3 broth
- Have colonies on A09-1(2) plate
Today
- Made 5ml overnight broths from 3 colonies on A09-1(2)
- Miniprepped pSB1C3
- 18.4 ng/ul
- Re-transformed vector DNA in DB3.1
- included control transformation in DH5a, should yield no colonies
- Prepared -20 glycerol stocks of knockouts JW3469 and JW3470.
