Team:Warsaw/Primers
From 2009.igem.org
(Difference between revisions)
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+ | <style> | ||
+ | td {height:40px} | ||
- | < | + | </style> |
+ | </html> | ||
+ | =Primers= | ||
+ | Here is the list of primers we have been using during our Project. | ||
- | + | ==cloning primers== | |
- | < | + | {|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;"| |
- | + | |- | |
- | < | + | !name |
- | < | + | !sequence |
- | <a name=" | + | !style="text-align: left;"|description |
- | <a name=" | + | |- |
- | <a name="invF">invF</a> | + | |width="10%"|<html><a name="croboxF">croboxF</a></html> |
- | <a name="invR">invR</a> | + | |ATCTAGATACCTCTGGCGGTGATACTAGTGT |
- | <a name="hlyF">hlyF</a> | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro-box cro-box] |
- | <a name="hlyR">hlyR</a> | + | |- |
- | <a name="croF">croF</a> | + | |<html><a name="croboxR">croboxR</a></html> |
- | <a name="croR">croR</a> | + | |ACACTAGTATCACCGCCAGAGGTATCTAGAT |
- | <a name="phoF">phoF</a> | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro-box cro-box] |
- | <a name="phoR">phoR</a> | + | |- |
- | <a name="mgtCF">mgtCF</a> | + | |<html><a name="invF">invF (-overhang)</a></html> |
- | <a name="mgtCR">mgtCR</a> | + | |ATCTAGATTATATTGACAGCGCACAGAGCGGGAA |
- | </ | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] |
- | + | |- | |
- | + | |<html><a name="invR">invR (-overhang)</a></html> | |
- | < | + | |GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT |
- | </ | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] |
- | </html> | + | |- |
+ | |<html><a name="invF">invF (+overhang)</a></html> | ||
+ | |GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
+ | |- | ||
+ | |<html><a name="invR">invR (+overhang)</a></html> | ||
+ | |CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
+ | |- | ||
+ | |<html><a name="hlyF">hlyF</a></html> | ||
+ | |TGTCTAGAATGAAAAAAATAATGCTAGTT | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin] | ||
+ | |- | ||
+ | |<html><a name="hlyR">hlyR</a></html> | ||
+ | |GACTAGTTTATTCGATTGGATTATCTA | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin] | ||
+ | |- | ||
+ | |<html><a name="croF">croF</a></html> | ||
+ | |ATCTAGAATGGAACAACGCATAACC | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro cro] | ||
+ | |- | ||
+ | |<html><a name="croR">croR</a></html> | ||
+ | |GTACTAGTGGGCTGGAATGTGTAAG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro cro] | ||
+ | |- | ||
+ | |<html><a name="phoF">phoF</a></html> | ||
+ | |ATCTAGAATGATGCGCGTACTGGTTGTAG | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ phoP/phoQ] | ||
+ | |- | ||
+ | |<html><a name="phoR">phoR</a></html> | ||
+ | |TTACTAGTGATGCGTGGAAGAACGCACAG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP/PhoQ] | ||
+ | |- | ||
+ | |<html><a name="mgtCF">mgtCF</a></html> | ||
+ | |ATCTAGATCCATTTTTTCTGGAAGAATAAG | ||
+ | |style="text-align: left;"|forward primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] | ||
+ | |- | ||
+ | |<html><a name="mgtCR">mgtCR</a></html> | ||
+ | |GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | ||
+ | |style="text-align: left;"|reverse primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] | ||
+ | |- | ||
+ | |<html><a name="p53F">p53F</a></html> | ||
+ | |GCTCTAGAATGGAGGAGCCGCAGTC | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] | ||
+ | |- | ||
+ | |<html><a name="p53R">p53R</a> </html> | ||
+ | |GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] | ||
+ | |- | ||
+ | |<html><a name="phoPF">phoPF</a> </html> | ||
+ | |GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATGCGCGTACTGGTTGTAGAG | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP] (new) | ||
+ | |- | ||
+ | |<html><a name="phoPR">phoPR</a> </html> | ||
+ | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTAGCGCAATTCAAAAAGATATCCTTGTCC | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP] (new) | ||
+ | |- | ||
+ | |<html><a name="phoQF">phoQF</a> </html> | ||
+ | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGAATAAATTTGCTCGCCATTTTCTGC | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoQ] (new) | ||
+ | |- | ||
+ | |<html><a name="phoQR">phoQR</a> </html> | ||
+ | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTCCTCTTTCTGTGTGGGATGCTGTC | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoQ] (new) | ||
+ | |- | ||
+ | |<html><a name="baxF">baxF</a> </html> | ||
+ | |ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#bax bax] | ||
+ | |- | ||
+ | |<html><a name="baxR">baxR</a> </html> | ||
+ | |ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#bax bax] | ||
+ | |- | ||
+ | |<html><a name="invF">invF</a> </html> | ||
+ | |GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] (with completely prefix and sufix) | ||
+ | |- | ||
+ | |<html><a name="invR">invR</a> </html> | ||
+ | |TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] (with completely prefix and sufix) | ||
+ | |- | ||
+ | |<html><a name="inlapsF">inlapsF</a> </html> | ||
+ | |GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalin A] | ||
+ | |- | ||
+ | |<html><a name="inlapsR">inlapsR</a></html> | ||
+ | |GCGCTGCAGTTATTTACTAGCACGTGCTT | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalin A] | ||
+ | |- | ||
+ | |<html><a name="JPSwiF">JPSwiF</a></html> | ||
+ | |TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | ||
+ | |style="text-align: left;"| | ||
+ | |- | ||
+ | |<html><a name="JPSwiRbez">JPSwiRbez</a></html> | ||
+ | |GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | ||
+ | |style="text-align: left;"| | ||
+ | |- | ||
+ | |<html><a name="JPSwiRGFP">JPSwiRGFP</a></html> | ||
+ | |GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG | ||
+ | |style="text-align: left;"| | ||
+ | |- | ||
+ | |<html><a name="mgtCnewF">mgtCnewF</a> </html> | ||
+ | |GAATTCGCGGCCGCTTCTAGAGTCCATTTTTTCTGGAAGAATAAG | ||
+ | |style="text-align: left;"|revised forward primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] (now with complete prefix) | ||
+ | |- | ||
+ | |<html><a name="mgtCnewR">mgtCnewR</a></html> | ||
+ | |CTGCAGCGGCCGCTACTAGTACCTCGTCATGTTGTTTTATTTTTTT | ||
+ | |style="text-align: left;"|revised reverse primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] (now with complete suffix) | ||
+ | |- | ||
+ | |<html><a name="YFPsilverF">YFPsilverF</a></html> | ||
+ | |GATGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAG | ||
+ | |style="text-align: left;"|forward primer for <partinfo>E0032</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="YFPsilverR">YFPsilverR</a></html> | ||
+ | |GCTCTGCAGCGGCCGCTACTAGTAGCTACTAAAGCGTAGTT | ||
+ | |style="text-align: left;"|reverse primer for <partinfo>E0032</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="mitoCOXsilverF">mitoCOXsilverF</a></html> | ||
+ | |TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | ||
+ | |style="text-align: left;"|forward primer for <partinfo>K177028</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="mitoCOXsilverR">mitoCOXsilverR</a></html> | ||
+ | |TTCCTGCAGCGGCCGCTACTAGTCAACGAATGGATCTTGGCGCGC | ||
+ | |style="text-align: left;"|reverse primer for <partinfo>K177028</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="p53silverF">p53silverF</a></html> | ||
+ | |CGAGAATTCGCGGCCGCTTCTAGAATGGAGGAGCCGCAGTC | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="p53silverR">p53silverR</a></html> | ||
+ | |TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="secr_silverF">secr_silverF</a></html> | ||
+ | |GGGAATTCCGCGGCCGCTTCTAGAGATGGCGGAGAAGGGAATG | ||
+ | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#Type_I_secretion_system hemolysin secretion signal] compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |<html><a name="secr_silverR">secr_silverR</a></html> | ||
+ | |CGGCTGCAGCGGCCGCTACTAGTTTATGCTGATGCTGTCAAAGTTATTG | ||
+ | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#Type_I_secretion_system hemolysin secretion signal] compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
+ | |- | ||
+ | |} | ||
{{WarFoot1}} | {{WarFoot1}} |
Latest revision as of 22:08, 20 October 2009
Primers
Here is the list of primers we have been using during our Project.
cloning primers
name | sequence | description |
---|---|---|
croboxF | ATCTAGATACCTCTGGCGGTGATACTAGTGT | forward primer for cro-box |
croboxR | ACACTAGTATCACCGCCAGAGGTATCTAGAT | reverse primer for cro-box |
invF (-overhang) | ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | forward primer for invasin |
invR (-overhang) | GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | reverse primer for invasin |
invF (+overhang) | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin |
invR (+overhang) | CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG | reverse primer for invasin |
hlyF | TGTCTAGAATGAAAAAAATAATGCTAGTT | forward primer for listeriolysin |
hlyR | GACTAGTTTATTCGATTGGATTATCTA | reverse primer for listeriolysin |
croF | ATCTAGAATGGAACAACGCATAACC | forward primer for cro |
croR | GTACTAGTGGGCTGGAATGTGTAAG | reverse primer for cro |
phoF | ATCTAGAATGATGCGCGTACTGGTTGTAG | forward primer for phoP/phoQ |
phoR | TTACTAGTGATGCGTGGAAGAACGCACAG | reverse primer for PhoP/PhoQ |
mgtCF | ATCTAGATCCATTTTTTCTGGAAGAATAAG | forward primer for the mgtc gene promoter |
mgtCR | GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | reverse primer for the mgtc gene promoter |
p53F | GCTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 |
p53R | GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | reverse primer for p53 |
phoPF | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATGCGCGTACTGGTTGTAGAG | forward primer for PhoP (new) |
phoPR | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTAGCGCAATTCAAAAAGATATCCTTGTCC | reverse primer for PhoP (new) |
phoQF | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGAATAAATTTGCTCGCCATTTTCTGC | forward primer for PhoQ (new) |
phoQR | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTCCTCTTTCTGTGTGGGATGCTGTC | reverse primer for PhoQ (new) |
baxF | ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | forward primer for bax |
baxR | ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | reverse primer for bax |
invF | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin (with completely prefix and sufix) |
invR | TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | reverse primer for invasin (with completely prefix and sufix) |
inlapsF | GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | forward primer for internalin A |
inlapsR | GCGCTGCAGTTATTTACTAGCACGTGCTT | reverse primer for internalin A |
JPSwiF | TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | |
JPSwiRbez | GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | |
JPSwiRGFP | GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG | |
mgtCnewF | GAATTCGCGGCCGCTTCTAGAGTCCATTTTTTCTGGAAGAATAAG | revised forward primer for the mgtc gene promoter (now with complete prefix) |
mgtCnewR | CTGCAGCGGCCGCTACTAGTACCTCGTCATGTTGTTTTATTTTTTT | revised reverse primer for the mgtc gene promoter (now with complete suffix) |
YFPsilverF | GATGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAG | forward primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
YFPsilverR | GCTCTGCAGCGGCCGCTACTAGTAGCTACTAAAGCGTAGTT | reverse primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
mitoCOXsilverF | TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | forward primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
mitoCOXsilverR | TTCCTGCAGCGGCCGCTACTAGTCAACGAATGGATCTTGGCGCGC | reverse primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
p53silverF | CGAGAATTCGCGGCCGCTTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
p53silverR | TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | reverse primer for p53 CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
secr_silverF | GGGAATTCCGCGGCCGCTTCTAGAGATGGCGGAGAAGGGAATG | forward primer for hemolysin secretion signal compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
secr_silverR | CGGCTGCAGCGGCCGCTACTAGTTTATGCTGATGCTGTCAAAGTTATTG | reverse primer for hemolysin secretion signal compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |