Team:Warsaw/Sources

From 2009.igem.org

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!style="text-align: center"|source
!style="text-align: center"|source
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|style="text-align: center"|[https://2009.igem.org/Team:Warsaw/Glossary#p53 p53]
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|style="text-align: center"|CDS of [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53]
|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016]
|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016]
|human<sup>4</sup>
|human<sup>4</sup>
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|style="text-align: center"|[https://2009.igem.org/Team:Warsaw/Glossary#bax bax]
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|style="text-align: center"|CDS of [https://2009.igem.org/Team:Warsaw/Glossary#bax bax]
|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027]
|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027]
|human <sup>5</sup>
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|style="text-align: center"|secretion system
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|style="text-align: center"|[https://2009.igem.org/Team:Warsaw/Glossary#Type_I_secretion_system secretion system]
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|''Escherichia coli''<sup>7</sup>
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<sup>6</sup> Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector
<sup>6</sup> Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector
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<sup>7</sup> Plasmids carrying essential elements of mentioned secretion system were a generous gift from ...
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<sup>7</sup> Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research
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Latest revision as of 23:45, 20 October 2009

Sources of natural bricks

Below we present sources of bricks prepared by our team.

Invasiveness bricks

Most of bricks connected with bacteria invasiveness were acquired from following organisms:

organism genomic sequence details acquired genes
Salmonella enterolitica ser. typhimurium LT21 [http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197] PhoP, PhoQ, MgtC promoter
Listeria monocytogenes EGD-E2 [http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210] listeriolysin, internalinA
Yersina enterolitica O:8 (8081)3 [http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415] invasin

Other bricks

name of the brick sequence details source
CDS of p53 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016] human4
CDS of bax [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027] human 5
mitochondrial targeting signal from the Cox gene [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177028 BBa_K177028] human6
secretion system Escherichia coli7


1 Genomic DNA from this strain was a generous gift from ...

2 Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, [http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=528&b_2501=5 Laboratory of Applied Microbiology], Faculty of Biology, University of Warsaw

3 Genomic DNA from this strain was a generous gift from ...

4 Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the [http://www.iimcb.gov.pl/zylicz_lab.php Lab of Molecular Biology], International Institute of Molecular and Cell Biology in Warsaw

5 Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the [http://www.igib.uw.edu.pl/index.php?id=80 Institute of Genetics and Biotechnology], University of Warsaw.

6 Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector

7 Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research