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Team:Wash U/Calendar-Home/1 July 2009
From 2009.igem.org
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-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow | -Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow | ||
| - | + | -Started new overnight cultures of biobrick parts and plasmids | |
Revision as of 16:09, 2 July 2009
-Miniprep #2 to obtain more biobrick DNA
-New gel with digested vs. undigested plasmids
-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow
-Started new overnight cultures of biobrick parts and plasmids
Miniprep #2:
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1) BBa_I15010 pSB2K3 2) BBa_E0240 pSB1A2 3) BBa_R0082 pSB1A2 4) plasmid pSB1T3 5) plasmid pSB1K3 6) BBa_B0034 pSB1A2 7) plasmid pSB1AT3 8) plasmid pSB1A3 9) plasmid pucHS27 10) plasmid pSB1AC3 11) BBa_B0015 pSB1AK3 12) plasmid pRKCBC3 |
158 ng/ul 210 ng/ul 62.5 ng/ul 325 ng/ul 280 ng/ul 145 ng/ul 665 ng/ul 308 ng/ul 275 ng/ul 338 ng/ul 168 ng/ul 272 ng/ul |
*Note: Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
