Team:Washington/Notebook/Flow Cytometry

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(New page: ==== Flow Cytometry ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, p...)
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Revision as of 04:03, 15 October 2009

Flow Cytometry

  1. Set up overnights of parts 48-51. Let grow overnight.
  2. Dilute 1 ul overnight into 1ml
  3. Add 1 mm IPTG and let grow for four hours
  4. After cells have grown up, place in flourophore (1 um) and allow time to bind (1 hour)
  5. Also place 1 ul beads in 1 ml along with 1 ul flourophore.
  6. Allow beads to bind to flourophore, then spin beads down, remove supernatent and replace with 1 ml water.
  7. Read the samples through the flow cytometer
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