Team:Washington/Notebook/Standard curve
From 2009.igem.org
Generating a Standard curve for GFP concentration
- Purify GFP-fused protein using traditional purification protocol
- Measure absorption at 280nM to quantify protein amount
- Serialy dilute protein into plate and read fluorescence
- Compare values of protein concentration to fluorescence to create a standard curve allowing for the comparison of fluorescence to protein level