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Template:Stanford Parts Table 0809
From 2009.igem.org
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<TR><TH>Part #</TH><TH>Name</TH><TH>Part Description</TH><TH>Type</TH></TR> | <TR><TH>Part #</TH><TH>Name</TH><TH>Part Description</TH><TH>Type</TH></TR> | ||
| - | <TR><TD>BBa_K223040</TD><TD>SoxR Gene</TD><TD>This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress.</TD><TD></TD></TR> | + | <TR><TD>BBa_K223040</TD><TD>SoxR Gene</TD><TD>This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress.</TD><TD>Basic</TD></TR> |
<TR><TD>Bba_K223041</TD><TD>SoxS promoter</TD><TD>The SoxS promoter is activated by the SoxR protein in the presence of oxidative stress, such as Paraquat or Nitric Oxide. It has a high affinity binding with the SoxR protein. It can be used in conjunction with the SoxR gene as an inducible sensor for oxidative stress. | <TR><TD>Bba_K223041</TD><TD>SoxS promoter</TD><TD>The SoxS promoter is activated by the SoxR protein in the presence of oxidative stress, such as Paraquat or Nitric Oxide. It has a high affinity binding with the SoxR protein. It can be used in conjunction with the SoxR gene as an inducible sensor for oxidative stress. | ||
| - | </TD><TD></TD></TR> | + | </TD><TD>Basic</TD></TR> |
<TR><TD>Bba_K223042</TD><TD>Blh Gene</TD><TD>The Blh gene cleaves beta-carotene into retinal. It can be used in the pathway for the production of retinoic acid from beta-carotene in conjunction with the RALDH II genes. | <TR><TD>Bba_K223042</TD><TD>Blh Gene</TD><TD>The Blh gene cleaves beta-carotene into retinal. It can be used in the pathway for the production of retinoic acid from beta-carotene in conjunction with the RALDH II genes. | ||
| - | </TD><TD></TD></TR> | + | </TD><TD>Basic</TD></TR> |
| - | <TR><TD>Bba_K223043</TD><TD>RALDH II Gene</TD><TD>The Raldh II gene converts retinal into retinoic acid. It is helpful if used in conjunction with the Blh gene for the entire process of converting beta-carotene into retinoic acid.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223043</TD><TD>RALDH II Gene</TD><TD>The Raldh II gene converts retinal into retinoic acid. It is helpful if used in conjunction with the Blh gene for the entire process of converting beta-carotene into retinoic acid.</TD><TD>Basic</TD></TR> |
| - | <TR><TD>Bba_K223044</TD><TD>SoxR-SoxS Promoter System</TD><TD>This entire system works as a superoxide sensor. Superoxides activate the SoxR protein to bind with the SoxS Promoter and begin transcription of downstream genes. It is an inducible sensor.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223044</TD><TD>SoxR-SoxS Promoter System</TD><TD>This entire system works as a superoxide sensor. Superoxides activate the SoxR protein to bind with the SoxS Promoter and begin transcription of downstream genes. It is an inducible sensor.</TD><TD>Composite</TD></TR> |
| - | <TR><TD>Bba_K223045</TD><TD>RBS- RALDH II - TT</TD><TD>Working composite part with a ribosome binding site and terminator.</TD><TD></TD></TR><TR><TD>Bba_K223046</TD><TD>SoxR/SoxS with GFP Reporter</TD><TD>SoxR - SoxS Promoter System with downstream GFP production. This was used to characterize the inducible oxidative stress signal when activating the SoxR-SoxS promoter system.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223045</TD><TD>RBS- RALDH II - TT</TD><TD>Working composite part with a ribosome binding site and terminator.</TD><TD>Intermediate</TD></TR> |
| - | <TR><TD>Bba_K223047</TD><TD>Lacl Promoter with SoxR/SoxS and GFP Reporter</TD><TD>This is the SoxR-SoxS inducible promoter system with downstream production of GFP in order to characterize the device. We used the lacl regulated promoter.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223046</TD><TD>SoxR/SoxS with GFP Reporter</TD><TD>SoxR - SoxS Promoter System with downstream GFP production. This was used to characterize the inducible oxidative stress signal when activating the SoxR-SoxS promoter system.</TD><TD>Composite</TD></TR> |
| - | <TR><TD>Bba_K223048</TD><TD>Blh and RALDH II Coupled Generator</TD><TD>This is the RBS- Blh- RBS- RALDH II- TT generator. Both of these parts are important in the cleaving of beta carotene into retinoic acid.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223047</TD><TD>Lacl Promoter with SoxR/SoxS and GFP Reporter</TD><TD>This is the SoxR-SoxS inducible promoter system with downstream production of GFP in order to characterize the device. We used the lacl regulated promoter.</TD><TD>Composite</TD></TR> |
| - | <TR><TD>Bba_K223049</TD><TD>SoxR - SoxS System with Blh and RALDH II system</TD><TD>This is the superoxide inducible SoxR-SoxS promoter system coupled with the production of Blh and RALDH II genes for the cleaving of beta carotene into retinoic acid.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223048</TD><TD>Blh and RALDH II Coupled Generator</TD><TD>This is the RBS- Blh- RBS- RALDH II- TT generator. Both of these parts are important in the cleaving of beta carotene into retinoic acid.</TD><TD>Intermediate</TD></TR> |
| - | <TR><TD>Bba_K223050</TD><TD>Lacl Promoter with SoxR - SoxS System with Blh and RALDH II system</TD><TD>Same as BBa_K223049 with the addition of an inducible lacl promoter.</TD><TD></TD></TR> | + | <TR><TD>Bba_K223049</TD><TD>SoxR - SoxS System with Blh and RALDH II system</TD><TD>This is the superoxide inducible SoxR-SoxS promoter system coupled with the production of Blh and RALDH II genes for the cleaving of beta carotene into retinoic acid.</TD><TD>Composite</TD></TR> |
| + | <TR><TD>Bba_K223050</TD><TD>Lacl Promoter with SoxR - SoxS System with Blh and RALDH II system</TD><TD>Same as BBa_K223049 with the addition of an inducible lacl promoter.</TD><TD>Composite</TD></TR> | ||
<TR><TD>Bba_K223051</TD><TD>Fill in</TD><TD></TD><TD></TD></TR> | <TR><TD>Bba_K223051</TD><TD>Fill in</TD><TD></TD><TD></TD></TR> | ||
<TR><TD>Bba_K223052</TD><TD>Fill in</TD><TD></TD><TD></TD></TR> | <TR><TD>Bba_K223052</TD><TD>Fill in</TD><TD></TD><TD></TD></TR> | ||
Revision as of 00:19, 19 October 2009
| Part # | Name | Part Description | Type |
|---|---|---|---|
| BBa_K223040 | SoxR Gene | This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress. | Basic |
| Bba_K223041 | SoxS promoter | The SoxS promoter is activated by the SoxR protein in the presence of oxidative stress, such as Paraquat or Nitric Oxide. It has a high affinity binding with the SoxR protein. It can be used in conjunction with the SoxR gene as an inducible sensor for oxidative stress. | Basic |
| Bba_K223042 | Blh Gene | The Blh gene cleaves beta-carotene into retinal. It can be used in the pathway for the production of retinoic acid from beta-carotene in conjunction with the RALDH II genes. | Basic |
| Bba_K223043 | RALDH II Gene | The Raldh II gene converts retinal into retinoic acid. It is helpful if used in conjunction with the Blh gene for the entire process of converting beta-carotene into retinoic acid. | Basic |
| Bba_K223044 | SoxR-SoxS Promoter System | This entire system works as a superoxide sensor. Superoxides activate the SoxR protein to bind with the SoxS Promoter and begin transcription of downstream genes. It is an inducible sensor. | Composite |
| Bba_K223045 | RBS- RALDH II - TT | Working composite part with a ribosome binding site and terminator. | Intermediate |
| Bba_K223046 | SoxR/SoxS with GFP Reporter | SoxR - SoxS Promoter System with downstream GFP production. This was used to characterize the inducible oxidative stress signal when activating the SoxR-SoxS promoter system. | Composite |
| Bba_K223047 | Lacl Promoter with SoxR/SoxS and GFP Reporter | This is the SoxR-SoxS inducible promoter system with downstream production of GFP in order to characterize the device. We used the lacl regulated promoter. | Composite |
| Bba_K223048 | Blh and RALDH II Coupled Generator | This is the RBS- Blh- RBS- RALDH II- TT generator. Both of these parts are important in the cleaving of beta carotene into retinoic acid. | Intermediate |
| Bba_K223049 | SoxR - SoxS System with Blh and RALDH II system | This is the superoxide inducible SoxR-SoxS promoter system coupled with the production of Blh and RALDH II genes for the cleaving of beta carotene into retinoic acid. | Composite |
| Bba_K223050 | Lacl Promoter with SoxR - SoxS System with Blh and RALDH II system | Same as BBa_K223049 with the addition of an inducible lacl promoter. | Composite |
| Bba_K223051 | Fill in | ||
| Bba_K223052 | Fill in | ||
| Bba_K223053 | Fill in | ||
| Bba_K223054 | Fill in | ||
| Bba_K223055 | Fill in | ||
| Bba_K223056 | Fill in | ||
| Bba_K223057 | Fill in | ||
| Bba_K223058 | Fill in | ||
| Bba_K223059 | Fill in | ||
| Bba_K223060 | Fill in |
