Template:Team:KULeuven/13 August 2009/BlueLightReceptor
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# Restriction digest {{kulpart|BBa_K145015}} cut with EcoRI and XbaI | # Restriction digest {{kulpart|BBa_K145015}} cut with EcoRI and XbaI | ||
# Electroporation of Psb3k3 + P1010 and the ligation of ... | # Electroporation of Psb3k3 + P1010 and the ligation of ... | ||
- | # Gelelectrophorese of {{Kulpart| | + | # Gelelectrophorese of {{Kulpart|BBa_K145015}} |
#* Only 1 band, so probably everything was cut | #* Only 1 band, so probably everything was cut | ||
- | # Gelectraction of {{Kulpart| | + | # Gelectraction of {{Kulpart|BBa_K145015}} |
#* Nanodrop = 29,3 ng/μl | #* Nanodrop = 29,3 ng/μl | ||
- | #ligation of vector {{Kulpart| | + | #ligation of vector {{Kulpart|BBa_K145015}} and insert |
Revision as of 09:29, 1 September 2009
- PCR product of 12-august of pairt
- Miniprep of
Nanodrop results:
Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
---|---|---|---|
123,3 | 1,82 | ||
141,2 | 1,99 |
- Restriction digest cut with EcoRI and XbaI
- Electroporation of Psb3k3 + P1010 and the ligation of ...
- Gelelectrophorese of
- Only 1 band, so probably everything was cut
- Gelectraction of
- Nanodrop = 29,3 ng/μl
- ligation of vector and insert