Template:Team:KULeuven/13 August 2009/VanillinReceptor

From 2009.igem.org

(Difference between revisions)
(New page: # VirB and RpoA miniprepped. Tomorow proceed with mutagenesis of RpoA # VirG redone (only TOPo was visible, 200bp) PCR from 2,3 and 1' # Α, Β, Γ, s pc purification to cre...)
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# VirB and RpoA miniprepped. Tomorow proceed with mutagenesis of RpoA
# VirB and RpoA miniprepped. Tomorow proceed with mutagenesis of RpoA
# VirG redone (only TOPo was visible, 200bp)  PCR from 2,3 and 1'
# VirG redone (only TOPo was visible, 200bp)  PCR from 2,3 and 1'
-
# Α, Β, Γ, s pc purification to create a TOPO vector tomorow
+
# α, β, γ, Δ pc purification to create a TOPO vector tomorow
Nanodrop:
Nanodrop:
  {| border ="1" align="center"
  {| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
|- align="center"
|- align="center"
-
| Α || 46,1 || 1,84 ||  
+
| α || 46,1 || 1,84 ||  
|- align="center"
|- align="center"
-
| Β || 112 || 1,82 ||   
+
| β || 112 || 1,82 ||   
|- align="center"
|- align="center"
-
| Γ || 207 || 1,85 ||   
+
| γ || 207 || 1,85 ||   
|- align="center"
|- align="center"
-
| S || 155,3 || 1,86 ||   
+
| Δ || 155,3 || 1,86 ||   
|-
|-
|}
|}

Revision as of 13:24, 14 August 2009

  1. VirB and RpoA miniprepped. Tomorow proceed with mutagenesis of RpoA
  2. VirG redone (only TOPo was visible, 200bp) PCR from 2,3 and 1'
  3. α, β, γ, Δ pc purification to create a TOPO vector tomorow

Nanodrop:

Part concentration (ng/μl) 260/280 λ 260/230 λ
α 46,1 1,84
β 112 1,82
γ 207 1,85
Δ 155,3 1,86
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