Template:Team:KULeuven/14 September 2009/BlueLightReceptor

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  1. miniprep of ligY colonies. nannodrop: 111 ng/µl and 116 ng/µl.
  2. restriction digest of ligY miniprep
  3. gelelectrophoresis of restricted ligY and compared to ligA. the ligY fragment was larger than ligA which means that the ligation Y fauled. the promotor region probably ligated to RFP which was not restricted out of the vector properly.
  4. gel extraction of plasmid pSB1A2 (nanodrop: 10ng/µl)
  5. a new ligY was performed
  6. control of pcr-product . It was ok.
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