Template:Team:KULeuven/17 September 2009/BlueLightReceptor
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(New page: # electroporation of logA (ligA in pBR322, a low copy plasmid). # a PCR on pSB3K3 with primers ...... # restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel ...)
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(New page: # electroporation of logA (ligA in pBR322, a low copy plasmid). # a PCR on pSB3K3 with primers ...... # restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel ...)
Newer edit →
Revision as of 09:15, 21 September 2009
- electroporation of logA (ligA in pBR322, a low copy plasmid).
- a PCR on pSB3K3 with primers ......
- restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel electrophoresis and extraction.
- ligations of ligA (blp+GFP) with pBR322 (logA) and pSB3K3 (likA) were put overnight
- was taken from the distribution plates and plated on LB
- liquid cultures of ligY (blp) were made
- restriction digest of ligY with EcoRI and PstI