Template:Team:KULeuven/21 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)

Revision as of 11:30, 14 September 2009

Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.

However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ech-plasmid. The signals do not correspond with an EF-ligation product.

The DNA was purified from the gel

Nanodrop results:

Part	ng/µl	260/280
sam8	2,5	1,14
sam5	7,9	1,60
ech	5,0	1,38
fcs	3,4	0,93

sam8 and sam5; fcs and ech were ligated

Used volumes:

Part	µl
sam8	34
sam5	6,3
ech	10,0
fcs	35,8

@@ Line 1: / Line 1: @@
 * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
-However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The signals do not correspond with an EF-ligation product.
+However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ''ech''-plasmid. The signals do not correspond with an EF-ligation product.
 * The DNA was purified from the gel
@@ Line 8: / Line 8: @@
 !| Part || ng/µl || 260/280 ||
 |- align="center"
-| Sam8 ||2,5 || 1,14 ||
+| ''sam8'' ||2,5 || 1,14 ||
 |- align="center"
-| Sam5 ||7,9 || 1,60 ||
+| ''sam5'' ||7,9 || 1,60 ||
 |- align="center"
-| Ech ||5,0 || 1,38 ||
+| ''ech'' ||5,0 || 1,38 ||
 |- align="center"
-| Fcs ||3,4 || 0,93 ||
+| ''fcs'' ||3,4 || 0,93 ||
 |-
 |}