Template:Team:KULeuven/27 August 2009/BlueLightReceptor

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  1. The electroporations of 26/08 were checked.
    • Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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had some colonies. they were ented in liquid culture.
    • LigC (Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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+ Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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) did not grow. we figured that an insert of 35bp was too short to ligate so we decided to use Status: 500 Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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as insert and Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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+ Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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as vector . The following restriction digest was started:
      • Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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was cut with SpeI and PstI
      • Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
BEGIN failed--compilation aborted at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8.

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was cut with XbaI and PstI  
  1. The miniprep that was made to sequence the LigA construct on 26/08 was used again to perform a restriction digest (EcoRI and PstI). This was put on gel to check whether there actually was LigA-insert in the vector and whether the insert had the right length. The gel showed a signal at 1000 bp and at 2000 bp which coincides with the insert (BLp + GFP) and the vector.
  2. A new setup to light the E.coli was engineered.
    • Fresh cultures were made from the old ones (LB plate ligA 14/08 and the two liquid cultures from 26/08 were used as templates.)
    • They were put in the 16°C room for about an hour
    • A blue light (40W) was put on them for about an hour
    • They were put in the 37°C incubator overnight