"
Template:Team:KULeuven/29 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) |
JochemDeen (Talk | contribs) |
||
| Line 1: | Line 1: | ||
* Split the restriction digest in 2 parts | * Split the restriction digest in 2 parts | ||
| - | ** 1 Part was directly used for ligation using double the mass required | + | ** 1 Part was directly used for ligation using double the mass required(Sams II and Ef II) |
{| border ="1" align="center" | {| border ="1" align="center" | ||
!| Part || DNA ng || volume µl | !| Part || DNA ng || volume µl | ||
| Line 14: | Line 14: | ||
|} | |} | ||
* The 2nd part was first put on gel electrophorese and purified before it was used for ligation | * The 2nd part was first put on gel electrophorese and purified before it was used for ligation | ||
| + | * Gel electrophorese of restriction on Sam, Sam8, fcs and ech | ||
| + | ** Sam5 and Sam8 showed expected results | ||
| + | ** fcs and ech was unexpected | ||
| + | ***fcs showed only 1 result on gel, expected two | ||
| + | ***ech showed 4 results in gel (all too short), expected one | ||
| + | ** Sam5 and Sam8 were purified and ligated (Sams I) | ||
| + | * Ech and Fcs were cut again and put overnight (overweekend...) in 16C | ||
Revision as of 09:52, 31 August 2009
- Split the restriction digest in 2 parts
- 1 Part was directly used for ligation using double the mass required(Sams II and Ef II)
| Part | DNA ng | volume µl |
|---|---|---|
| Sam5 | 100 | 0,8 |
| Sam8 | 126,4 | 2,7 |
| Ech | 100 | 1 |
| Fcs | 244 | 2,2 |
- The 2nd part was first put on gel electrophorese and purified before it was used for ligation
- Gel electrophorese of restriction on Sam, Sam8, fcs and ech
- Sam5 and Sam8 showed expected results
- fcs and ech was unexpected
- fcs showed only 1 result on gel, expected two
- ech showed 4 results in gel (all too short), expected one
- Sam5 and Sam8 were purified and ligated (Sams I)
- Ech and Fcs were cut again and put overnight (overweekend...) in 16C
